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<div class="">Dear Colleagues,</div>
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<div class="">Over the past few years I have shared with a number of people the details of our method for nanofabricating holey gold or holey carbon grids (Marr 2014, JSB 185, 42-7, which was based on Chester 2007, Ultramicroscopy 187, 685-91). I’ve lost track
of how many people are using this method and I now want to share a two important updates:</div>
<div class="">First, Zhijie Li in the Rini lab at U of T made an e-beam master pattern with 1.2 um holes. We find that on baking the resulting plastic films, this pattern gives high quality ~2 um holes that are superior to what we could get from the patterns
with smaller holes that we used previously. Patterns can be ordered from the Toronto Nanofabrication Centre (<a href="mailto:alex.tsukernik@utoronto.ca" class="">alex.tsukernik@utoronto.ca</a>)</div>
<div class="">Second, we learned from TTP Labtech (with whom we shared the details of our manufacturing process) that using a liftoff agent prior to making the PDMS stamps dramatically improves the quality of the stamps. The details of the liftoff agent can
be found in comments section below the youtube video describing the process (<a href="https://www.youtube.com/watch?v=ycsdCSmD4rw" class="">https://www.youtube.com/watch?v=ycsdCSmD4rw</a>).</div>
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<div class="">Best,</div>
<div class="">John</div>
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-- <br class="">
John Rubinstein<br class="">
Molecular Medicine Program<br class="">
The Hospital for Sick Children Research Institute<br class="">
686 Bay Street, Rm. 20-9705<br class="">
Toronto, ON<br class="">
Canada<br class="">
M5G 0A4<br class="">
Tel: (+001) 416-813-7255<br class="">
Fax: (+001) 416-813-5022<br class="">
<a href="http://www.rubinsteinlab.org" class="">www.rubinsteinlab.org</a></div>
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