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<p class="MsoNormal"><span lang="EN-US" style="color:black">Dear colleagues:</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> <span class="apple-converted-space"> </span></span><b><u><span lang="EN-US" style="font-family:"Times New Roman Bold";color:black">Fully-funded doctoral position at the School of Biological Sciences University of Auckland</span></u></b><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="font-family:"Times New Roman",serif;color:black">We are seeking a highly motivated candidate to carry out structure/function studies of component proteins of anti-feeding prophage (Afp), a bacteriophage tail-like particle using high-resolution
cryo-EM. The project is in collaboration with Dr. Mark Hurst of NZ Ag Research and funded by a grant from Ministry of Business, innovation and Employment from the NZ govt. </span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-GB" style="font-family:"Times New Roman",serif;color:black">The bacteria derived anti-feeding prophage (Afp) is a unique DNA-free entity likened to a cell-free type 6 secretion system. It is pathogenic to a biological pest, the New Zealand pasture
(grass grub) by transporting and delivering a protein toxin to the grass grub larvae. In order to assess the mechanism of Afp assembly and target cell interaction, using structural, molecular and cell biology, we seek to determine and demonstrate the mechanism
of Afp cell targeting and toxin delivery to the insect grass grub host. [</span><i><span lang="EN-US" style="font-family:"Times New Roman",serif;color:black">(1) Hurst et al. (2004)<span class="apple-converted-space"> </span><u>J. Bacteriol</u>.<a name="_ENREF_21"> 186:5116-5128;
2) Hurst et al. (2007)<span class="apple-converted-space"> </span><u>FEMS Microbiol. Lett.</u><span class="apple-converted-space"> </span>270:42-48</a>), (3) Heymann, J. B., Bartho, J. D., Rybakova, D. et al. (2013)<span class="apple-converted-space"> </span><u>J.
Biol. Chem.</u><span class="apple-converted-space"> </span>288: 25276-25284. (4) Rybakova, D., Radjainia, M., et al. (2013)<span class="apple-converted-space"> </span><u>Mol. Microbiol.</u><span class="apple-converted-space"> </span>89: 702-714. (5)<span class="apple-converted-space"> </span></span></i><i><span lang="EN-GB" style="font-family:"Times New Roman",serif;color:black">Rybakova,
D., Schramm, P., Mitra, A. K., and Hurst, M. R. H.<span class="apple-converted-space"> </span>(2015).<span class="apple-converted-space"> </span><u>Mol. Microbiol</u>.<span class="apple-converted-space"> </span>96:815-826)</span></i><span lang="EN-GB" style="font-family:"Times New Roman",serif;color:black">]</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:36.0pt;font-variant-caps: normal;orphans: auto;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-US" style="font-family:"Times New Roman",serif;color:black">The selected candidate should have background in biophysics or protein structural biochemistry with M.Sc. GPA near 8 (out of 10). Experience in Structural Biology and transmission electron
microscopy will be preferred. Familiarity with recombinant protein expression and/or X-ray crystallography will be a plus.</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:36.0pt;font-variant-caps: normal;orphans: auto;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-US" style="font-family:"Times New Roman",serif;color:black">The cryo-EM facility at UoA is the premier such facility at New Zealand. We have a FEI T120 (cryo) for routine work and a TF20 equipped with GIF for high-resolution cryo-EM and cryo-tomography
work. We have direct access to the Titan Krios facilities at Monash University Australia and to a similar facility at Wollongong University through a strategic research linkage. The computing facility within our laboratory and the access to computing cluster
at UoA is sufficient for large-volume image processing. We also have access to an International class X-ray crystallography facility in house and ready access to the Australian Synchrotron facility..</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:36.0pt;font-variant-caps: normal;orphans: auto;widows: auto;-webkit-text-size-adjust: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span lang="EN-US" style="font-family:"Times New Roman",serif;color:black">University of Auckland has a vibrant research environment and is the premier such Institute in New Zealand. Auckland is a multi-ethnic cosmopolitan city consistently ranked amongst the
top ten cities in the world. New Zealand is well known for its scenic beauty.<span class="apple-converted-space"> </span>For further information, please send a current CV and contact Dr. Alok K. Mitra, School of Biological Sciences, University of Auckland,
Auckland, New Zealand. Tel: +64 09 923 8162, E.mail: </span><u><span lang="EN-US" style="font-family:"Times New Roman",serif;color:purple"><a href="mailto:a.mitra@auckland.ac.nz"><span style="color:purple">a.mitra@auckland.ac.nz</span></a></span></u><span class="apple-converted-space"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:purple"> </span></span><span lang="EN-US" style="font-family:"Times New Roman",serif;color:black">or
Dr. Mark H. Hurst,<span class="apple-converted-space"> </span></span><span lang="EN-GB" style="font-family:"Times New Roman",serif;color:black">Forage Science AgReserach Lincoln, New Zealand Tel +64 03 3259919, E-mail:</span><span lang="EN-GB" style="font-family:"Arial",sans-serif;color:black"> </span><u><span lang="EN-GB" style="font-family:"Arial",sans-serif;color:#954F72"><a href="mailto:mark.hurst@agresearch.co.nz"><span style="color:#954F72">mark.hurst@agresearch.co.nz</span></a></span></u><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-GB" style="font-family:"Times New Roman",serif;color:black"> </span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> Kind regards</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> Alok K. Mitra, PhD</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> School of Biological Sciences</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> Academic Leader, cryo-EM facility</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> University of Auckland</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> Auckland</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> New Zealand</span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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<span lang="EN-US" style="color:black"> </span><span lang="EN-GB" style="color:black"><o:p></o:p></span></p>
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