<div dir="auto">hi again,<div dir="auto"><br></div><div dir="auto">We operate the Polara and the F20 in microprobe, with small C2 apertures [50um for the F20, and 30um for the Polara, if i remember well...] and we have the feeling is pretty close to parallel. Win Hagen reported in twitter a under 2A reconstruction of apo-fe in microprobe AND FalconII in linear mode...so... </div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 14, 2019, 18:56 Craig Yoshioka <<a href="mailto:yoshiokc@ohsu.edu" rel="noreferrer noreferrer" target="_blank">yoshiokc@ohsu.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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Oh, I have one comment about nP vs uP. Yes, you can get a smaller parallel beam in nP -but- it is also way more sensitive to changes in beam size in nP. As a result, we have been able to get below 3Å on an Arctica in microprobe, even when the beam size was
not set to be perfectly parallel- it remains fairly close over a pretty large range. This is a way more convenient configuration for a lot of use.
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<div>On Feb 14, 2019, at 11:57 AM, Craig Yoshioka <<a href="mailto:yoshiokc@ohsu.edu" rel="noreferrer noreferrer noreferrer" target="_blank">yoshiokc@ohsu.edu</a>> wrote:</div>
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<div>Hi Gary,</div>
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We haven’t exhaustively tested yet, but we have been targeting around 15-20eps on the K3 and have been getting nice results. This general range was based on the 400 -> 1500 read rate increase.
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<div>At very high-magnifications 15eps is quite a high flux, so sometimes we will go below 10eps just so that we don’t have to push very high FPS (40+) with < 1 sec exposures. We have seen some mild pattern noise occasionally creep into images when
trying to save at high frame rates. Not sure yet if this is just our K3, or if it also seen by others. So far it doesn’t seem to be a problem in practice, but feedback from others if they have seen this would be appreciated.
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<div>Cheers,</div>
<div>-Craig</div>
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<div>On Feb 14, 2019, at 11:45 AM, Garry P Morgan <<a href="mailto:Garry.Morgan@Colorado.EDU" rel="noreferrer noreferrer noreferrer" target="_blank">Garry.Morgan@Colorado.EDU</a>> wrote:</div>
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thanks everyone. <br>
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<div>On Feb 14, 2019, at 11:53 AM, Reyes, Francis <<a href="mailto:francis.reyes@thermofisher.com" rel="noreferrer noreferrer noreferrer" target="_blank">francis.reyes@thermofisher.com</a>> wrote:</div>
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<div><span style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;float:none;display:inline!important">Yes
it is advisable to work in nanoprobe. At parallel illumination and a 50 um C2 you should have around a 1.8um illuminated area.</span>
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<div dir="ltr"><span style="background-color:rgba(255,255,255,0)">Francis Reyes, Ph.D<br>
Engineer III, Field Applications<br>
Materials & Structural Analysis<br>
<br>
Thermo Fisher Scientific<br>
<a dir="ltr" rel="noreferrer noreferrer noreferrer">5350 NE Dawson Creek Drive</a></span>
<div><span style="background-color:rgba(255,255,255,0)"><a dir="ltr" rel="noreferrer noreferrer noreferrer">Hillsboro,
OR 97124</a> </span>
<div><span style="background-color:rgba(255,255,255,0)">Phone - <a href="tel:+1%20(720)%20322-6150" dir="ltr" rel="noreferrer noreferrer noreferrer" target="_blank">+1 (720) 322-6150</a></span></div>
<div><span style="background-color:rgba(255,255,255,0)"><a href="mailto:francis.reyes@thermofisher.com" dir="ltr" rel="noreferrer noreferrer noreferrer" target="_blank">francis.reyes@thermofisher.com</a></span></div>
<div><span style="background-color:rgba(255,255,255,0)"><a href="http://thermofisher.com/" dir="ltr" rel="noreferrer noreferrer noreferrer" target="_blank">thermofisher.com</a></span></div>
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On Feb 14, 2019, at 12:36 PM, Garry P Morgan <<a href="mailto:Garry.Morgan@colorado.edu" rel="noreferrer noreferrer noreferrer" target="_blank">Garry.Morgan@colorado.edu</a>> wrote:<br>
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<div dir="ltr"><span>CAUTION: This email originated from outside of the organization. Do not click links or open attachments unless you recognize the sender and know the content is safe.</span><br>
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<span>Hello all,</span><br>
<span></span><br>
<span>i’m looking for some info on the optimal dose rate for acquiring K3 movie stacks. i would appreciate it if any of you could send me your optimum acquisition parameters for imaging cry-samples for SPA…ie, dose rate, best frame rate/number of
frames, etc.</span><br>
<span></span><br>
<span>i’m also trying to figure out how to setup my low dose so that we have parallel illumination for our record images (on an FEI Tecnai F20). the problem i see is that in microprobe the beam is spread much too wide to image/expose a single hole
at a time at our record mag. is it advisable to work in nano probe to get a smaller beam that is parallel? any advice on this would be appreciated.</span><br>
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<span>thanks,</span><br>
<span>Garry</span><br>
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<span>________________________________</span><br>
<span>Garry Morgan</span><br>
<span>Director — Boulder EM Services — Department of MCD Biology</span><br>
<span>Campus Box 347 — University of Colorado — Boulder, CO 80309</span><br>
<span>phone: 303-492-8402</span><br>
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