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<p>Dear Guobin,</p>
<p>stated like this, it is to difficult to give an "absolute"
recommendation. A first question would be if you see the Thon
rings by eye? If not, I would say that most programs will not be
able to find the defocus. If you can see them, then it is a matter
of trying. All I can offer in this regard is to try Xmipp on this,
if you make one or two of these micrographs available, you may
send me a personal message with the link and we will check them.</p>
<p>Kind regards, Carlos Oscar<br>
</p>
<div class="moz-cite-prefix">El 14/02/2019 a las 21:40, Guobin Hu
escribió:<br>
</div>
<blockquote type="cite"
cite="mid:774657896.1137089.1550176824857@mail.yahoo.com">
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<div>Dear Cryo-EM folks,</div>
<div><br>
</div>
<div>I am dealing with some micrographs of very low defocus values
e.g. a few tens of nanometers. I found it sometimes is difficult
for the programs I am using to find the right defocus value even
I set the corresponding defocus search range. I see a lot of CTF
estimation programs on the wikipage. I do not want to download
all of them and try one by one. I would greatly appreciate it if
somebody can recommend one that works well with lowly defocused
micrographs. The best is the program can also display the Thon
rings of CTF of very small defocus values. </div>
<div><br>
</div>
<div>Best,</div>
<div>Guobin Hu </div>
<br>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
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</blockquote>
<pre class="moz-signature" cols="72">--
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano e-mail: <a class="moz-txt-link-abbreviated" href="mailto:coss@cnb.csic.es">coss@cnb.csic.es</a>
Biocomputing unit <a class="moz-txt-link-freetext" href="http://i2pc.es/coss">http://i2pc.es/coss</a>
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco) Tlf: 34-91-585 4510
28049 MADRID (SPAIN) Fax: 34-91-585 4506
------------------------------------------------------------------------
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