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Takanori, yes, I used the <span style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; background-color: rgb(255, 255, 255); display: inline !important">non-converged
half maps (i.e.<span> </span></span><br style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; background-color: rgb(255, 255, 255)">
<span style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; background-color: rgb(255, 255, 255); display: inline !important">run_itNNN_halfX_class001_unfil.mrc)
for post processing to get those Relion results. </span></span><br>
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<p style="margin-top: 0px; margin-bottom: 0px;">Brenda Gonzalez</p>
<p style="margin-top: 0px; margin-bottom: 0px;">PhD Candidate</p>
<p style="margin-top: 0px; margin-bottom: 0px;">Department of Biological Sciences – Purdue University</p>
<p style="margin-top: 0px; margin-bottom: 0px;">Purdue SACNAS Chapter President</p>
<p style="margin-top: 0px; margin-bottom: 0px;">AGEP Scholar</p>
<p style="margin-top: 0px; margin-bottom: 0px;">e: gonzalb@purdue.edu</p>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of 3dem-request@ncmir.ucsd.edu <3dem-request@ncmir.ucsd.edu><br>
<b>Sent:</b> Monday, February 11, 2019 3:00 PM<br>
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<b>Subject:</b> 3dem Digest, Vol 138, Issue 26</font>
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1. Re: unmasked FSC different (Takanori Nakane)<br>
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Message: 1<br>
Date: Mon, 11 Feb 2019 19:24:31 +0000<br>
From: Takanori Nakane <tnakane@mrc-lmb.cam.ac.uk><br>
To: 3dem@ncmir.ucsd.edu<br>
Subject: Re: [3dem] unmasked FSC different<br>
Message-ID: <8343c2ca-8f7c-c6ef-aa93-4e3ac59b1137@mrc-lmb.cam.ac.uk><br>
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Hi,<br>
<br>
Didn't you use non-converged half maps (i.e. <br>
run_itNNN_halfX_class001_unfil.mrc)<br>
for PostProcesss?<br>
<br>
Best regards,<br>
<br>
Takanori Nakane<br>
<br>
On 2019/02/11 18:57, Brenda Gonzalez wrote:<br>
> Hello,<br>
> <br>
> I was wondering if anyone has noticed issues with their FSC results using Relion.<br>
> <br>
> For a while, I have been getting results where my unmasked FSC curve floats above 0. However, when I looked at the model.star file FSC data and plotted that manually, the unmasked FSC curve actually appears to reach 0.<br>
> <br>
> Does anyone know what could be causing this or how I could fix it?<br>
> <br>
> Thank you,<br>
> <br>
> <br>
> <br>
> Brenda Gonzalez<br>
> <br>
> Department of Biological Sciences ? Purdue University<br>
> <br>
> e: gonzalb@purdue.edu<br>
> <br>
> <br>
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