<div dir="auto"><div class="gmail_quote" dir="auto"><div dir="ltr"><br></div><div dir="auto"><div class="gmail_quote" dir="auto"><div dir="auto"><span style="font-family:sans-serif;font-size:12.8px">Hi Cheri,</span><div dir="auto" style="font-family:sans-serif;font-size:12.8px">We recently experienced exactly the same problem with the two latest purchased boxes of quantifoil gold R2/2 grids. We used the same glow discharge machine (ELMO) and the same settings that we normally use and we experienced that when we put the grids in a buffer like PBS or HEPES + 2% PFA the carbon was detaching on almost all the grids.</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">To see where the problem could come from we used two very old batches of quantifoil grids and repeated the same procedure , but in this case the carbon remained nicely attached to the grids.</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><br></div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">So it could be that your problem might not be due to your plasma cleaner but rather to the new way quantifoil is manifacturing their grids. </div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><br></div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"> I contacted quantifoil and sent them the corresponding pictures, they told me that in fact they recently changed their carbon film deposition parameters (he talked about different plasma cleaner conditions...) and they told me that they would look into it , but I haven t heard back from them yet. </div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">It would be great if you could also contact them to report your problem.</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">I hope this helps,</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">Ciao </div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">Anna</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><br></div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">-------------------------------------------------</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><br></div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">Anna Sartori-Rupp , PhD</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">Ultrastructural Bioimaging Unit</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px">Institut Pasteur,</div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><span style="font-size:12.8px">28 rue du Dr Roux</span><br></div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><span style="font-size:12.8px">75015 </span><span style="font-size:12.8px">Paris, France </span></div><div dir="auto" style="font-family:sans-serif;font-size:12.8px"><br></div></div><br><div class="gmail_quote"><div dir="ltr">Il giorno Gio 6 Dic 2018, 9:12 PM HAMPTON, CHERI M CTR USAF AFMC AFRL/RXAS <<a href="mailto:cheri.hampton.ctr@us.af.mil" rel="noreferrer noreferrer" target="_blank">cheri.hampton.ctr@us.af.mil</a>> ha scritto:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p style="margin-top:0;margin-bottom:0">Hi all,</p>
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<p style="margin-top:0;margin-bottom:0">I have suddenly begun experiencing the carbon detaching from my QF R2/1 grids when submersed in buffer and when applying proteins for blotting and cryo-plunging. I have tried two different lots of grids, and now I am
suspicious of the facility's plasma cleaner that I have been using to glow discharge. All settings and timings have remained constant on my part. I plan to seek out another plasma cleaner to do a comparison test, but meanwhile, what kinds of solvents or materials
placed in the plasma cleaner/glow discharge unit might linger and cause problems? I'd like to justify having a "clean" one just for TEM :)<br>
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<p style="margin-top:0px;margin-bottom:0px">Thanks for your input,</p>
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<div>Cheri</div>
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~'~'~'~'~'~'~'~'~'~'~</div>
<div>Cheri M. Hampton, PhD</div>
<div>Research Microscopist</div>
<div>UES, Inc.<br>
AFRL/RXAS<br>
2179 12th St, B652/R122<br>
Wright-Patterson AFB, OH 45433-7750<br>
(937) 255-9081<br>
<a href="mailto:cheri.hampton.ctr@us.af.mil" rel="noreferrer noreferrer noreferrer" target="_blank">cheri.hampton.ctr@us.af.mil</a> <br>
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