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<p style="margin-top:0; margin-bottom:0">Hi all,</p>
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<p style="margin-top:0; margin-bottom:0">I have suddenly begun experiencing the carbon detaching from my QF R2/1 grids when submersed in buffer and when applying proteins for blotting and cryo-plunging. I have tried two different lots of grids, and now I am
suspicious of the facility's plasma cleaner that I have been using to glow discharge. All settings and timings have remained constant on my part. I plan to seek out another plasma cleaner to do a comparison test, but meanwhile, what kinds of solvents or materials
placed in the plasma cleaner/glow discharge unit might linger and cause problems? I'd like to justify having a "clean" one just for TEM :)<br>
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<p style="margin-top:0px; margin-bottom:0px">Thanks for your input,</p>
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<div>Cheri</div>
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~'~'~'~'~'~'~'~'~'~'~</div>
<div>Cheri M. Hampton, PhD</div>
<div>Research Microscopist</div>
<div>UES, Inc.<br>
AFRL/RXAS<br>
2179 12th St, B652/R122<br>
Wright-Patterson AFB, OH 45433-7750<br>
(937) 255-9081<br>
cheri.hampton.ctr@us.af.mil <br>
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