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<p style="margin-top:0;margin-bottom:0">Hey Cheri</p>
<p style="margin-top:0;margin-bottom:0">Did you see the same thing happen to a different brand of carbon grids? Like a Cflat or Ted pellas?
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<p style="margin-top:0;margin-bottom:0">Sometimes QF grids have a bad box of grids thats all. And the plasma cleaner may actually be fine. Unfortunately if thats the case you will just have to discard the entire box and move on to the next one. If you have
the time to spare, you can reach out to QF with some documentation and proof that you got a faulty box of grids and they usually send a replacement.
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<p style="margin-top:0;margin-bottom:0">Just sharing my two cents</p>
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<p style="margin-top:0;margin-bottom:0">Hope this helps</p>
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<p style="margin-top:0;margin-bottom:0">Best<br>
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<p>Amar Parvate</p>
<p>Ollmann-Saphire lab</p>
TSRI<br>
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<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of HAMPTON, CHERI M CTR USAF AFMC AFRL/RXAS <cheri.hampton.ctr@us.af.mil><br>
<b>Sent:</b> Thursday, December 6, 2018 2:47 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] carbon detaching from grids</font>
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<p style="margin-top:0; margin-bottom:0">Hi all,</p>
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<p style="margin-top:0; margin-bottom:0">I have suddenly begun experiencing the carbon detaching from my QF R2/1 grids when submersed in buffer and when applying proteins for blotting and cryo-plunging. I have tried two different lots of grids, and now I am
suspicious of the facility's plasma cleaner that I have been using to glow discharge. All settings and timings have remained constant on my part. I plan to seek out another plasma cleaner to do a comparison test, but meanwhile, what kinds of solvents or materials
placed in the plasma cleaner/glow discharge unit might linger and cause problems? I'd like to justify having a "clean" one just for TEM :)<br>
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<p style="margin-top:0px; margin-bottom:0px">Thanks for your input,</p>
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<div>Cheri</div>
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~'~'~'~'~'~'~'~'~'~'~</div>
<div>Cheri M. Hampton, PhD</div>
<div>Research Microscopist</div>
<div>UES, Inc.<br>
AFRL/RXAS<br>
2179 12th St, B652/R122<br>
Wright-Patterson AFB, OH 45433-7750<br>
(937) 255-9081<br>
cheri.hampton.ctr@us.af.mil <br>
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