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<p class="MsoNormal"><span style="color:black">Dear colleagues:<o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">We would like to announce that as part of the ongoing expansion of the free “Getting Started in Cryo-EM” video series, we have just made a large set of new training videos public covering the operation of Arctica and Titan Krios microscopes.
More specifically, we begin with a comprehensive introduction to every control panel and button on the user interface, then cover gun alignment, the condenser system, how to obtain parallel illumination on both the Arctica and Krios, consequences of not having
a parallel beam, different ways to focus, projection and diffraction issues, history and differences between all kinds of detectors, issues surrounding energy filtration, how to quickly assess the performance of your microscope, what to look for when screening
samples, and how to set up EPU for single particle data collection. The demonstration videos are full screen captures of the monitors plus views of the hand panels, so viewers can see all the actions taken by the operator in sequence and their consequences,
while they are being explained in real time. In addition, the demonstration videos are complemented with many new lecture-style theory videos presented through drawings and images on a tablet, as in the original Getting Started in Cryo-EM lectures.<o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">Together the whole package is now ~70 hours of video, which we hope you will find helpful in your training. <o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">The videos are all released free of charge with the Creative Commons license CC BY 4.0, so you can download, copy, redistribute, remix, and otherwise use as you see fit as long as you credit the original source. Their production was
funded in part by the NIH Transformative High Resolution Cryo-Electron Microscopy Program (grant R25 EY-029128 to Grant Jensen) and in part by ThermoFisher Scientific.<o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">Because there are now so many videos, we have arranged them in different modules on a customized website that keeps track of your progress and that will allow us to add self-test questions in the future. Please find the videos at<o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black"><a href="https://em-learning.com"><span style="color:purple">https://em-learning.com</span></a><o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">As for many other NIH- and/or company-funded resources, the website requires a simple registration to help us gather statistical information about who is watching the videos. Please also send us (either Matthijn or me, both copied
above) email directly about any suggestions, comments or corrections you have – this will help us improve the videos and will give us the feedback we need for our reports to the NIH. <o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">Enjoy!<o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black">Matthijn Vos and Grant Jensen<o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<span style="color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">--------------------------------------------------------------------------<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">Grant Jensen<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">Professor of Biology and Biophysics and Investigator, HHMI<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">California Institute of Technology, Mail code 114-96<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">1200 E. California Blvd.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">Pasadena, CA 91125<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">626-395-8827 (phone)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">626-395-5730 (fax)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">JensenATcaltech.edu<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">http://www.jensenlab.caltech.edu<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">office location: 359 Broad (northwest corner of campus)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">Administrative Assistant Karin Mallard
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">626-395-8893 (phone)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">kmallardATcaltech.edu<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;color:black">--------------------------------------------------------------------------<o:p></o:p></span></p>
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