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<p class="MsoNormal">Hello Bradford,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have a T20 with a LaB6 filament and an Eagle. You will have no problem running SerialEM on the microscope computer as long as you have the FEI scripting interface installed. Most systems do, and SerialEM website explains how to determine
this. Setting up the software may take some time, from hours to days, depending on experience. The resolution will be limited by the thermionic gun (i.e., low spatial coherency and brightness) and the camera. This configuration will not be able to produce
high-resolution (by modern standards) structures. Perhaps about 15 A is a realistic rough estimate. However, it will perform well as a screening microscope and for negative-stain single particle data collection.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best regards,<o:p></o:p></p>
<p class="MsoNormal">Yaroslav<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Yaroslav Tsybovsky, Ph.D. (Contractor)<o:p></o:p></p>
<p class="MsoNormal">Scientist II<o:p></o:p></p>
<p class="MsoNormal">Frederick National Laboratory for Cancer Research<o:p></o:p></p>
<p class="MsoNormal">Leidos Biomedical Research, Inc. <o:p></o:p></p>
<p class="MsoNormal">Post Office Box B<o:p></o:p></p>
<p class="MsoNormal">Frederick, Maryland 21702<o:p></o:p></p>
<p class="MsoNormal">Phone: 301-846-7550<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Ross, Bradford <bradford.ross@botany.ubc.ca> <br>
<b>Sent:</b> Wednesday, October 10, 2018 4:54 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Setting up a 200kV Tecnai with 4K Eagle for SPA: Worth bothering?<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Hello 3DEM Community,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">We have a 200kV Tecnai G2 LaB6 TEM equipped with an Eagle 4K camera, Gatan 626 cryo holder, and a Vitrobot Mk IV. This instrument gets used fairly routinely for
Cryo TEM of lipid nanoparticles, ambient temp TEM Tomography, and HRTEM of various types of nanoparticles down to approx. 1.4A crystal lattice resolution.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Lately I've been thinking about setting the system up to acquire single particle cryo EM data to expand our capabilities. However, I know that the newer systems
such as Talos Arctica, Glacios, Krios etc. with direct electron detectors are far more capable than what we have. Further, our Tecnai is currently completely running in a Windows environment, so that complicates things a bit with the SPA software packages
all running on Linux. Are there folks out there running similar systems for this purpose? Do you set the microscope PC up to dual boot with Linux for single particle acquisition, or use the support PC? I have no experience in single particle acquisition or
analysis, but lots of experience doing low dose cryo TEM and tinkering with our lab equipment.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">In short, would it be worth the effort of reconfiguring the system to acquire datasets that may be sub-par by today's standards? Or perhaps a useful hands-on experience
in learning about how cryo SPA works, and expanding the capability of our lab?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Cheers,<o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black">Bradford Ross<br>
<br>
Electron Microscopy Technician<br>
BioImaging Facility<br>
University of British Columbia<br>
Cunningham Building Rm. 64<br>
2146 East Mall <br>
Vancouver, B.C. <br>
V6T 1Z4<br>
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phone 604-822-6996<o:p></o:p></span></p>
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