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<p class="MsoNormal"><span style="font-size:11.0pt">Dear List,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Please pass on this opportunity to any interested graduate students or post-docs.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Description<o:p></o:p></span></b></p>
<p class="MsoNormal"><span style="font-size:11.0pt">A full-time, five-year cryo-EM post-doctoral research associate position funded by a recent NIH U01 award to study the structural biology of alpha-synuclein is immediately available in the Center for Cellular
Imaging under the mentorship of Dr. James Fitzpatrick at Washington University School of Medicine in St. Louis, MO, USA. The position is focused on the use of single-particle and tomographic cryo-EM approaches to elucidate the atomic structure of alpha-synuclein
(Asyn) fibrils isolated and amplified from different regions of pathological brain tissue. The position will involve preparation of vitrified Asyn fibrils in collaboration with the Kotzbauer Lab in the Department of Neurology, along with the acquisition and
analysis of both single-particle and tomographic datasets acquired on a Titan Krios platform. We are explicitly seeking candidates who have a strong background and training in using cryo-EM approaches, with skills in both wet lab sample purification and vitrification,
computational analyses using RELION for helical reconstruction and macromolecular model fitting using Coot.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Required Qualifications<o:p></o:p></span></b></p>
<ul style="margin-top:0in" type="disc">
<li class="MsoNormal" style="mso-list:l0 level1 lfo1"><span style="font-size:11.0pt">Ideal postdoctoral candidates will have a strong background in biochemistry as well as training / expertise in cryo-EM approaches (both data acquisition and analysis).<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l0 level1 lfo1"><span style="font-size:11.0pt">Experience in structural biology approaches are also desirable, as well as strong verbal and written communication skills.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l0 level1 lfo1"><span style="font-size:11.0pt">The appointee has earned a Ph.D., M.D. or equivalent terminal or doctoral degree.<o:p></o:p></span></li></ul>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Preferred Qualifications<o:p></o:p></span></b></p>
<ul style="margin-top:0in" type="disc">
<li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Experience in single-particle cryo-EM helical reconstruction using RELION.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Experience in cryo-electron tomography reconstructions using IMOD and/or Tomo3D.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Experience with macromolecular model building in cryo-EM density maps in Coot.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Demonstrated computer skills including proficiency with a scripting language (e.g. Perl, Python).<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Experience in working in a Linux/UNIX environment, experience with cluster computing is a plus.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Knowledge, background, or experience in structural biology approaches.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Excellent verbal and written communication skills.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Excellent analytical, interpersonal, organizational, and time-management skills.<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo2"><span style="font-size:11.0pt">Self-motivated and ability to work effectively in a team environment.<o:p></o:p></span></li></ul>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Base pay is commensurate with experiment and position is eligible for full-time benefits.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Applicants should send a CV, brief description of current research activities, and contact information of 3 references to:<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">James Fitzpatrick, Ph.D.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Associate Professor, Neuroscience and Cell Biology & Physiology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Director, Center for Cellular Imaging<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Washington University School of Medicine<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">660 S. Euclid Avenue, Campus Box 8108<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">St Louis, MO 63110<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Email: <a href="mailto:fitzp@wustl.edu">fitzp@wustl.edu</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">-- <o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt">James A.J. Fitzpatrick, Ph.D.<o:p></o:p></span></b></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Scientific Director, Washington University Center for Cellular Imaging<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Associate Professor, Cell Biology & Physiology, Neuroscience and Biomedical Engineering<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Schools of Medicine and Engineering and Applied Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Washington University in St. Louis<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">660 S. Euclid Avenue, Campus Box 8108<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">St Louis, MO 63110<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Tel: +1 314-747-0838<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Fax: +1 314-362-3563<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Email: <a href="mailto:fitzp@wustl.edu">
<span style="color:#0563C1">fitzp@wustl.edu</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">Web: <a href="http://wucci.wustl.edu">
<span style="color:#0563C1">http://wucci.wustl.edu</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><u><span style="font-size:10.0pt">Shipping Address<o:p></o:p></span></u></p>
<p class="MsoNormal"><span style="font-size:10.0pt">WU Center for Cellular Imaging<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">4523 Clayton Avenue<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt">St. Louis, MO 63110<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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