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Dear all,<br>
<br>
I found previous posts on Gatan multi-specimen cryo holder 910.<br>
We are now considering purchasing this holder and am curious about
the current situation. Were all these problems already fixed? <br>
Any information is greatly appreciated.<br>
<br>
Best, <br>
<br>
<br>
Tomohiro Nishizawa<br>
University of Tokyo<br>
<br>
<br>
<br>
<br>
<div class="moz-cite-prefix">On 2017/11/01 0:17, Matthias Wolf
wrote:<br>
</div>
<blockquote type="cite"
cite="mid:C835B06D-51F9-4D37-84BA-AC363D05C6B4@oist.jp">
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<div><span></span></div>
<div>
<div>Dear Valorie,</div>
<div><br>
</div>
<div>Thank you very much for your detailed reply. This is indeed
very helpful.</div>
<div><br>
</div>
<div>But I don’t fully understand the anatomy of he holder -
When you talk about the “cassette”, what exactly do you mean?
From the brochure it looks just like a 626 with 3 wells at the
tip of the holder rod, into which a naked grid is loaded
directly and secured by split ring (presumably a more
“springy” version of the ring used in the 626). This would be
consistent with your “old design”. Does the entire tip of the
holder come off?</div>
<div><br>
</div>
<div>Or are you loading assembled FEI cartridges (ring, grid,
C-clip) unsecured onto the holder and those can fall out?</div>
<div>Their height would probably be too thick for locking them
down with the split ring. I remember that silicon nitride
grids, which are about 0.4 micron thick, could barely be
clipped in the 626.</div>
<div>If so, is there a dedicated version of the 910, which is
designed to take FEI cartridges (the “new design”)?</div>
<div><br>
</div>
<div>Also, how many hours of working time do you get before
refilling the dewar?</div>
<div>Is a movie rate camera required for drift compensation at
anything but low mag?</div>
<div><br>
</div>
<div>Thanks, Leo, for pointing out a newer model - I will
inquire.</div>
<div><br>
</div>
<div>Best regards,</div>
<div><br>
</div>
<div> Matthias </div>
<div><br>
On Oct 31, 2017, at 21:37, Bowman, Valorie D <<a
href="mailto:vdb@purdue.edu" moz-do-not-send="true">vdb@purdue.edu</a>>
wrote:<br>
<br>
</div>
<blockquote type="cite">
<div>
<p>Dear Matthias,</p>
<p><br>
</p>
<p>We have one. It is both great and terrifying. I have
used it to screen 6 cryo conditions in an afternoon. BUT,
it has one awful design flaw- it is incredibly easy to
drop the cassette into the column. If a person is looking
at the 3rd grid and turns the grid selector the wrong
direction, & plop! Gravity immediately pulls the left
end of the cassette down off the edge of the stage, the
right end pivots up off the ball on the end of the rod,
and the whole thing falls out into the abyss.
<br>
</p>
<p><br>
</p>
<p>We have one of the older models, and that design was far
superior. Loading was more difficult, but this risk does
not exist with the grids mounted in wells in the sample
rod itself. </p>
<p><br>
</p>
<p>Both models take 15-20 minutes longer to stabilize in the
microscope after insertion, and they take longer to cool
down, but I count that a fair trade off for 3 samples at
once. The split-ring clip rings are not too
different from the 626 version; it's just easier to
accidentally push through a grid.</p>
<p><br>
</p>
<p>By far, the worst thing is the lack of a real "lock" onto
the cartridges. This holder was tested by one of our best
people, and he dropped the cartridge in the scope the very
first time he used it! I know it is only a matter of time
before it happens again-- and the cartridges are not
cheap, plus potential scope down time if it blocks the
beam, pole pieces, etc.</p>
<p><br>
</p>
<p>It's a great holder, but if you have a wide range of
levels of users, it might not be one for everyone.</p>
<p><br>
</p>
<p>Valorie<br>
</p>
<p> <br>
</p>
<div id="Signature">
<div name="divtagdefaultwrapper"
style="font-family:Calibri,Arial,Helvetica,sans-serif;
font-size:; margin:0">
<div style="font-family:Tahoma; font-size:13px">
<div style="font-family:Tahoma; font-size:13px"><i>Valorie
Bowman</i><br>
<br>
<a href="mailto:vdb@purdue.edu"
moz-do-not-send="true">vdb@purdue.edu</a><br>
494-5643<br>
EM Facility Laboratory Manager/<br>
Senior Research Electron Microscopist<br>
Purdue Cryo EM Facility<br>
Purdue University</div>
</div>
</div>
</div>
<div style="color: rgb(33, 33, 33);">
<hr tabindex="-1" style="display:inline-block; width:98%">
<div id="divRplyFwdMsg" dir="ltr"><font
style="font-size:11pt" face="Calibri, sans-serif"
color="#000000"><b>From:</b> 3dem <<a
href="mailto:3dem-bounces@ncmir.ucsd.edu"
moz-do-not-send="true">3dem-bounces@ncmir.ucsd.edu</a>>
on behalf of Matthias Wolf <<a
href="mailto:matthias.wolf@oist.jp"
moz-do-not-send="true">matthias.wolf@oist.jp</a>><br>
<b>Sent:</b> Monday, October 30, 2017 11:00 PM<br>
<b>To:</b> <a href="mailto:3dem@ncmir.ucsd.edu"
moz-do-not-send="true">3dem@ncmir.ucsd.edu</a><br>
<b>Subject:</b> [3dem] Gatan 910 multi specimen cryo
transfer holder</font>
<div> </div>
</div>
<div>
<div class="WordSection1">
<p class="MsoNormal">Hi all,</p>
<p class="MsoNormal"> </p>
<p class="MsoNormal">We are considering the purchase
of a Gatan 910 cryo transfer multi specimen side
entry holder.</p>
<p class="MsoNormal"> </p>
<p class="MsoNormal">Does anyone have experience with
this holder?</p>
<p class="MsoNormal">The specs with respect to
stability are identical to the 626 (the
alternative), but I don’t know how it will perform
in practice. It can take 3 grids.</p>
<p class="MsoNormal"> </p>
<p class="MsoNormal">The holder will be used for
screening of a larger number of cryo samples. Cryo
tomography is not a requirement. Due to possible
contamination during unloading, the idea is not to
reuse screened grids – once plunging conditions are
established, a second grid frozen under identical
conditions on a reproducible plunging bot will be
used for data collection on a higher end cryo TEM
with DED and autoloader. We hope to accelerate the
cryo screening by loading 3 grids at once instead of
one at a time and to reduce frosting and ice
contamination with less cryo transfers. This step
aims at offloading screening from the expensive high
end tools and to maximize their beam time for
productive data collection. The holder will also be
used at room temperature for negative stained
samples before cryo screening, both of which will
act as triage steps for samples of insufficient
quality.</p>
<p class="MsoNormal"> </p>
<p class="MsoNormal"> Matthias</p>
</div>
</div>
</div>
</div>
</blockquote>
</div>
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