<html><head><style type='text/css'>p { margin: 0; }</style></head><body><div style='font-family: times new roman,new york,times,serif; font-size: 12pt; color: #000000'><div>Dear 3DEM,</div><div><br></div><div>I'm working on a Talos and the FEG emission history is highly inconsistent and currently lower than I'd like for single particle data collection on a Falcon II camera. I'm hoping FEI Talos owners would be willing share with me their FEG emission levels. I'd also like to know what dose rate you can achieve under parallel illumination conditions at your preferred data collections: gun lens, spot size, extraction voltage, etc. <br></div><div><br></div><div>The values I'm seeing are ~30-45uA and under parallel conditions it's difficult to achieve better than 10 e-/Ang per second with spot size 3 or greater.<br></div><div><br></div><div>Kind Regards,</div><div>Duncan</div><div><br></div><div><br></div><div>Duncan Sousa, PhD<br> Johns Hopkins University<br> School of Medicine<br> 725 N. Wolfe Street, Hunterian 716<br> Baltimore, MD 21205-2185<br> 1-410-614-1335 </div></div></body></html>