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Dear Julien,
<div class="">It’s a good idea, to check that your sample will adsorb to GrOx, and perhaps you’d also like to see how it’s oriented. </div>
<div class="">However, things might look different between a negative stain protocol versus blotting and plunge freezing. It’s probably better to move quickly to freezing, as that’s where optimisation can take time, and you can see how your sample interacts
with GrOx under the conditions you’ll use for eventual data collection. In other words, diagnose GrOx at stage 3 of this scheme: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5140023/figure/F1/" class="">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5140023/figure/F1/</a> </div>
<div class="">If neg stain on GrOx turns out to be challenging, it will be a waste of time for likely no benefit over regular grids.</div>
<div class="">Negative stain on regular carbon grids can tell you if your particle looks the right shape & size, and what the concentration is like. (A rule of thumb some use to start, is having found a good concentration in neg stain, 10x for cryo in holes,
and a smaller increase for cryo on support films.)</div>
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<div class="">All the best</div>
<div class="">Teige</div>
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<blockquote type="cite" class="">
<div class="">On 11 Sep 2018, at 16:55, Julien Bous <<a href="mailto:julien.bous@etu.umontpellier.fr" class="">julien.bous@etu.umontpellier.fr</a>> wrote:</div>
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<p class="">Dear Community,</p>
<p class=""><span id="p115" class=""><span id="131" class="">I</span>'m PhD student in
<span id="120" class="">cryoEM</span> and <span id="132" class="">I</span> <span id="211-212" class="">
plan</span> to <span id="213-214" class="">use</span> <span id="134" class="">graphene</span>
<span id="137" class="">oxide</span> grids for a complicate sample.</span><span id="p228" class=""> Therefore, in order to make a first assessment of the interaction between the graphene oxide and my sample, I want to try in first to do a negative stain on
graphene oxide with my sample Has anyone ever seen or tried this , I didn't find any bibliography?</span><span id="p229" class=""> And is it looks like a good idea for more experienced peoples?</span></p>
<p class=""><span id="p229" class="">Thank you very much for your time.</span></p>
<p class=""><span id="p229" class="">Best,</span></p>
<p class=""><span id="p229" class="">Julien<br class="">
</span></p>
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