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<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D">We have the
<span class="SpellE">Pelco</span> <span class="SpellE">EasiGlow</span><span style="mso-spacerun:yes">
</span>glow discharge apparatus, separate from a <span class="SpellE">Cressington</span> carbon coater (with which I do not have experience).<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D">The
<span class="SpellE">EasiGlow</span> has worked without problems for many years<span style="mso-spacerun:yes">
</span>(9<span style="mso-spacerun:yes"> </span>years or more), and users love the easy operation, with a single button to push.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D">The only issue is that
some users are a bit impatient and try to yank off the bell jar before the vacuum is fully released and doing so chip the edge of the bell jar (which then does not seal).– Filling the chipped area with JB Weld and sanding it smooth fixed it many times, but
eventually we had to buy a new jar.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><span style="mso-spacerun:yes"> </span><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D">Go Spartans!<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D">Regards,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D">Stan<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">Dr. Stanislav Vitha
<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">Research Scientist<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">Microscopy and Imaging Center<span style="mso-spacerun:yes">
</span><br>
Texas A&M University <o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">301 Old Main Dr<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">College Station, TX 77843-2257<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">Office: ILSB Room 1131<a href="http://microscopy.tamu.edu/"><font color="blue"><span style="color:blue"><br>
</span></font></a>Tel. 979-845-1607<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt;mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D;mso-no-proof:yes">http://microscopy.tamu.edu<br style="mso-special-character:line-break">
<![if !supportLineBreakNewLine]><br style="mso-special-character:line-break">
<![endif]><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="3" color="#1f497d" face="Calibri"><span style="font-size:12.0pt;mso-bidi-font-size:11.0pt;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";color:#1F497D"><o:p> </o:p></span></font></p>
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<p class="MsoNormal"><b><font size="2" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman";font-weight:bold">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";mso-fareast-font-family:"Times New Roman"">
3dem [mailto:3dem-bounces@ncmir.ucsd.edu] <b><span style="font-weight:bold">On Behalf Of
</span></b>Parent, Kristin<br>
<b><span style="font-weight:bold">Sent:</span></b> Friday, September 07, 2018 12:37 PM<br>
<b><span style="font-weight:bold">To:</span></b> 3dem@ncmir.ucsd.edu<br>
<b><span style="font-weight:bold">Subject:</span></b> [3dem] questions about carbon coaters /glow discharge<o:p></o:p></span></font></p>
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<p class="MsoNormal"><font size="3" face="Calibri"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Hello all,
<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">I was hoping some of you might share good/bad experiences with a carbon coater & glow discharge device? We are planning to purchase one to support our growing facility and I would
love to hear this groups’ experience. <o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">We have on hand a plasma cleaner that works really well, so what I am hoping for is ideally something to supplement our existing equipment (for those that prefer glow discharge)
and also is capable of carbon coating. <o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Thank you in advance,
<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Kristin
<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="1" face="Calibri"><span style="font-size:9.0pt">-- </span></font><font size="1" color="black" face="Arial"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black"> </span></font><font size="1"><span style="font-size:9.0pt"><o:p></o:p></span></font></p>
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<p class="MsoNormal"><font size="1" color="black" face="Arial"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Kristin N. Parent, Ph.D.<br>
J. K. Billman, Jr., M.D. Endowed Research Professor</span></font><font size="1"><span style="font-size:8.0pt">
</span></font><font size="1"><span style="font-size:9.0pt"><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="1" face="Calibri"><span style="font-size:9.0pt">and
</span></font><font size="1" color="black" face="Arial"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Assistant Professor of Biochemistry & Molecular Biology<br>
Michigan State University<br>
603 Wilson Road<br>
East Lansing, MI 48824</span></font><font size="1"><span style="font-size:9.0pt"><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="1" color="black" face="Arial"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Office phone: (517) 432-8434</span></font><font size="1" color="black"><span style="font-size:9.0pt;color:black"><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="1" color="black" face="Arial"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__kparentlab.natsci.msu.edu_&d=DwMGaQ&c=ODFT-G5SujMiGrKuoJJjVg&r=tE-7MR_zhznDCk27UIOmF3KCvigbMQUT9WjrucIisxY&m=AeieE4STXjyYpq7quSIuHQT-42yy1OnpTXbLyBrQbUs&s=msFGECctI8t4RODwkc4-PTKfaS1Yiybkn57L725GuwU&e=">https://kparentlab.natsci.msu.edu/</a><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="1" color="black" face="Arial"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Twitter: @Phage4Lyfe<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="1" color="black" face="Calibri"><span style="font-size:9.0pt;color:black"><o:p> </o:p></span></font></p>
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<p class="MsoNormal"><font size="3" face="Calibri"><span style="font-size:12.0pt"><o:p> </o:p></span></font></p>
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