<div dir="auto"><div dir="auto"></div>*strikes match*<div dir="auto"><br></div><div dir="auto">Dithering is used in astronomy to increase the sampling rate beyond physical Nyquist of detector. This works by moving the detector between successive images, by fractional amounts of the pixel dimension.</div><div dir="auto"><br></div><div dir="auto">*pours petrol*</div><div dir="auto"><br></div><div dir="auto">So really, we're not strictly limited to even Nyquist sampling. A sufficient but not necessary condition on achievable resolution.</div><div dir="auto"><br></div><div dir="auto">*room explodes*</div><div dir="auto"><br></div><div dir="auto">Thanks to Jason K for pointing this out! </div><div dir="auto"><br></div><div dir="auto">Ben H.</div><div dir="auto"><br></div><div dir="auto"><br></div><div dir="auto"><br></div><div dir="auto"><br></div><div dir="auto"><br></div><div dir="auto"><br></div><div dir="auto">Ben<br><div class="gmail_quote" dir="auto"><div dir="ltr">On Sun, Sep 2, 2018, 11:57 AM <<a href="mailto:3dem-request@ncmir.ucsd.edu" rel="noreferrer noreferrer noreferrer" target="_blank">3dem-request@ncmir.ucsd.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Send 3dem mailing list submissions to<br>
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Today's Topics:<br>
<br>
1. Re: GroEL best resolution map. (FSC RESOLUTION ??)<br>
(Marin van Heel)<br>
2. Re: GroEL best resolution map. (FSC RESOLUTION ??)<br>
(Vojtech Prazak)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Sun, 2 Sep 2018 10:05:53 -0300<br>
From: Marin van Heel <<a href="mailto:marin.vanheel@googlemail.com" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">marin.vanheel@googlemail.com</a>><br>
To: Sjors Scheres <<a href="mailto:scheres@mrc-lmb.cam.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">scheres@mrc-lmb.cam.ac.uk</a>>, Natesh Ramanathan<br>
<<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a>>, <a href="mailto:3dem@ncmir.ucsd.edu" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">3dem@ncmir.ucsd.edu</a><br>
Cc: "<a href="mailto:CCPEM@JISCMAIL.AC.UK" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">CCPEM@JISCMAIL.AC.UK</a>" <<a href="mailto:CCPEM@JISCMAIL.AC.UK" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">CCPEM@JISCMAIL.AC.UK</a>><br>
Subject: Re: [3dem] GroEL best resolution map. (FSC RESOLUTION ??)<br>
Message-ID: <<a href="mailto:eb904df8-d5f6-2454-52c3-a13f2a6d24cd@googlemail.com" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">eb904df8-d5f6-2454-52c3-a13f2a6d24cd@googlemail.com</a>><br>
Content-Type: text/plain; charset="utf-8"; Format="flowed"<br>
<br>
<br>
Dear Sjors,<br>
<br>
It is not a matter of software implementation but it is a fundamental <br>
theoretical basis of signal processing and the Fourier transform!<br>
You seem to have misunderstood the discussion on the issue here on <br>
3DEM/CCPEM mailers a while ago (June 2016?). It is NOT about "routinely <br>
achieve resolutions very close to Nyquist" but rather about "to which <br>
level you are ALLOWED to claim/interpret the significant resolution" <br>
based on an FSC curve.<br>
Maybe I need to write a separate paper about the matter to better <br>
explain the fundamental principles behind it.<br>
<br>
This "under-sampling problem" is an important matter because - <br>
especially in combination with erroneous resolution thresholds like the <br>
"gold standard FSC 0.143" (based on "sloppy statistics" see our bioRxiv <br>
paper)" - it makes it impossible to objectively compare the resolution <br>
levels achieved in independent experiments.? That makes efforts to <br>
perform projects like "resolution challenges" futile and not worth <br>
wasting your time on.<br>
<br>
Cheers,<br>
Marin<br>
<br>
===============<br>
<br>
On 02/09/2018 07:14, Sjors Scheres wrote:<br>
> Dear Natesh,<br>
> The limitation of 3x the pixel size of the maximum reachable <br>
> resolution might be true for software written by Marin, but it is <br>
> definitely not true for many other programs, including relion. We <br>
> routinely achieve resolutions very close to Nyquist, which is only 2x <br>
> the pixel size.<br>
><br>
> Hth,<br>
> Sjors<br>
><br>
> Sent from my phone.<br>
><br>
> -------- Original message --------<br>
> From: Marin van Heel <<a href="mailto:marin.vanheel@googlemail.com" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">marin.vanheel@googlemail.com</a>><br>
> Date: 01/09/2018 19:43 (GMT+01:00)<br>
> To: Natesh Ramanathan <<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a>>, <a href="mailto:3dem@ncmir.ucsd.edu" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">3dem@ncmir.ucsd.edu</a><br>
> Subject: Re: [3dem] GroEL best resolution map. (FSC RESOLUTION ??)<br>
><br>
><br>
> Dear Natesh,<br>
><br>
> I just checked the technical data of the EMD-3407 dataset and I saw <br>
> that its pixel size is 1.05A . That means that no resolution levels <br>
> better than 3 x 1.05A = 3.15A? may be claimed for processing results <br>
> based on that data set, following the standards we set in the past <br>
> (for the younger generation: I am the main inventor of the FSC ;) ). <br>
> Going beyond a resolution claim of 3.15A would mean that the dataset <br>
> is undersampled, and would thus require a new data collection with a <br>
> smaller pixel size. For a possible resolution claim of, say, 2A? you <br>
> need a maximum pixel size of 0.66A !? Moreover, the 0.143 threshold <br>
> FSC threshold used in this EMD-3407 case is itself wrong, while based <br>
> on sloppy statistics. <br>
> (<a href="https://www.biorxiv.org/content/early/2017/11/24/224402).Bottom" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">https://www.biorxiv.org/content/early/2017/11/24/224402).Bottom</a> <br>
> line:? to compare the results of different cryo-EM experiments you <br>
> need correct resolution criteria applied to sufficiently fine sampled <br>
> cryo-EM data.<br>
><br>
> Cheers<br>
><br>
> Marin<br>
><br>
> On 01/09/2018 12:57, Natesh Ramanathan wrote:<br>
>> Dear All,<br>
>><br>
>> ? ? ? Does anyone know/have a GroEL cryoEM map with resolution? 2.5 ? <br>
>> or better?<br>
>> I see that the best resolution in EMDataBank is 3.26 ??- EMD-3407 and <br>
>> EMD-3415.<br>
>> ? ? ? If you have one, is it possible to share the map with me?<br>
>><br>
>> Many thanks.<br>
>> Best regards,<br>
>> Natesh<br>
>> -- <br>
>> ----------------------------------------------------------<br>
>> "Live Simply and do Serious Things .. "<br>
>> - Dorothy Mary Crowfoot Hodgkin OM, FRS<br>
>><br>
>> "In Science truth always wins"<br>
>> - Max Ferdinand Perutz OM FRS<br>
>> ----------------------------------------------------------<br>
>> Dr. Ramanathan Natesh<br>
>> Assistant Professor,<br>
>> School of Biology,<br>
>> Indian Institute of Science Education and Research Thiruvananthapuram <br>
>> (IISER-TVM),<br>
>> Maruthamala P.O., Vithura,<br>
>> Thiruvananthapuram,? 695551, Kerala, India<br>
>><br>
>> <a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a> <mailto:<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a>><br>
>> <a href="http://www.researcherid.com/rid/C-4488-2008" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">http://www.researcherid.com/rid/C-4488-2008</a><br>
>> ORCID: <a href="http://orcid.org/0000-0002-1145-5962" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">http://orcid.org/0000-0002-1145-5962</a><br>
>> <a href="https://publons.com/author/1520837/ramanathan-natesh#profile" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">https://publons.com/author/1520837/ramanathan-natesh#profile</a><br>
>> <a href="http://faculty.iisertvm.ac.in/natesh" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">http://faculty.iisertvm.ac.in/natesh</a><br>
>><br>
>> Office Ph. 0091- 471-2778087<br>
>><br>
>><br>
>> _______________________________________________<br>
>> 3dem mailing list<br>
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>> <a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>
><br>
><br>
> -- <br>
> ==============================================================<br>
><br>
> Prof Dr Ir Marin van Heel<br>
><br>
> Laborat?rio Nacional de Nanotecnologia - LNNano<br>
> CNPEM/LNNano, Campinas, Brazil<br>
><br>
> tel: +55-19-3518-2316<br>
> mobile +55-19-983455450 (current)<br>
> mobile +55-19-981809332<br>
> (041-19-981809332 TIM)<br>
> Skype: Marin.van.Heel<br>
> email: marin.vanheel(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a><br>
> marin.vanheel(A_T)<a href="http://lnnano.cnpem.br" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">lnnano.cnpem.br</a><br>
> and: mvh.office(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a><br>
><br>
> --------------------------------------------------<br>
> Emeritus Professor of Cryo-EM Data Processing<br>
> Leiden University<br>
> Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)<br>
> --------------------------------------------------<br>
> Emeritus Professor of Structural Biology<br>
> Imperial College London<br>
> Faculty of Natural Sciences<br>
> email: m.vanheel(A_T)<a href="http://imperial.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">imperial.ac.uk</a><br>
> --------------------------------------------------<br>
><br>
> I receive many emails per day and, although I try,<br>
> there is no guarantee that I will actually read each incoming email.<br>
<br>
<br>
-- <br>
==============================================================<br>
<br>
Prof Dr Ir Marin van Heel<br>
<br>
Laborat?rio Nacional de Nanotecnologia - LNNano<br>
CNPEM/LNNano, Campinas, Brazil<br>
<br>
tel: +55-19-3518-2316<br>
mobile +55-19-983455450 (current)<br>
mobile +55-19-981809332<br>
(041-19-981809332 TIM)<br>
Skype: Marin.van.Heel<br>
email: marin.vanheel(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a><br>
marin.vanheel(A_T)<a href="http://lnnano.cnpem.br" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">lnnano.cnpem.br</a><br>
and: mvh.office(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a><br>
<br>
--------------------------------------------------<br>
Emeritus Professor of Cryo-EM Data Processing<br>
Leiden University<br>
Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)<br>
--------------------------------------------------<br>
Emeritus Professor of Structural Biology<br>
Imperial College London<br>
Faculty of Natural Sciences<br>
email: m.vanheel(A_T)<a href="http://imperial.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">imperial.ac.uk</a><br>
--------------------------------------------------<br>
<br>
I receive many emails per day and, although I try,<br>
there is no guarantee that I will actually read each incoming email.<br>
<br>
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------------------------------<br>
<br>
Message: 2<br>
Date: Sun, 02 Sep 2018 16:56:41 +0100<br>
From: "Vojtech Prazak" <<a href="mailto:vojta@strubi.ox.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">vojta@strubi.ox.ac.uk</a>><br>
To: <a href="mailto:3dem@ncmir.ucsd.edu" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">3dem@ncmir.ucsd.edu</a>, "Natesh Ramanathan" <<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a>>,<br>
"Sjors Scheres" <<a href="mailto:scheres@mrc-lmb.cam.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">scheres@mrc-lmb.cam.ac.uk</a>>, "Marin van Heel"<br>
<<a href="mailto:marin.vanheel@googlemail.com" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">marin.vanheel@googlemail.com</a>><br>
Cc: <a href="mailto:ccpem@jiscmail.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">ccpem@jiscmail.ac.uk</a><br>
Subject: Re: [3dem] GroEL best resolution map. (FSC RESOLUTION ??)<br>
Message-ID: <<a href="mailto:2f313702c62808321b3a318c44e71347@strubi.ox.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">2f313702c62808321b3a318c44e71347@strubi.ox.ac.uk</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
<br>
Dear Marin,<br>
<br>
This topic is clearly important for you but I think you will find it has nothing to do with Natesh's original question.? I'm sure Sjors will be thrilled to continue this discussion in a private thread.<br>
<br>
Best,<br>
<br>
Vojta<br>
<br>
<br>
<br>
Vojt?ch Pra??k<br>
Graduate Student<br>
Department of Structural Biology<br>
The Wellcome Trust Centre for Human Genetics <br>
Roosevelt Drive, Oxford, OX3 7BN UK <br>
University of Oxford<br>
<br>
Email: <a href="mailto:vojta@strubi.ox.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">vojta@strubi.ox.ac.uk</a><br>
Web: <a href="https://www.strubi.ox.ac.uk/research/kay-grunewald" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">https://www.strubi.ox.ac.uk/research/kay-grunewald</a><br>
?<br>
<br>
-----Original Message-----<br>
From: "Marin van Heel" <<a href="mailto:marin.vanheel@googlemail.com" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">marin.vanheel@googlemail.com</a>><br>
To: "Sjors Scheres" <<a href="mailto:scheres@mrc-lmb.cam.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">scheres@mrc-lmb.cam.ac.uk</a>>, "Natesh Ramanathan" <<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a>>, <a href="mailto:3dem@ncmir.ucsd.edu" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">3dem@ncmir.ucsd.edu</a><br>
Cc: <a href="mailto:CCPEM@JISCMAIL.AC.UK" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">CCPEM@JISCMAIL.AC.UK</a><br>
Date: 09/02/18 14:07<br>
Subject: Re: [3dem] GroEL best resolution map. (FSC RESOLUTION ??)<br>
<br>
<br>
Dear Sjors,<br>
<br>
It is not a matter of software implementation but it is a fundamental theoretical basis of signal processing and the Fourier transform!<br>
You seem to have misunderstood the discussion on the issue here on 3DEM/CCPEM mailers a while ago (June 2016?). It is NOT about "routinely achieve resolutions very close to Nyquist" but rather about "to which level you are ALLOWED to claim/interpret the significant resolution" based on an FSC curve. <br>
Maybe I need to write a separate paper about the matter to better explain the fundamental principles behind it.<br>
<br>
This "under-sampling problem" is an important matter because - especially in combination with erroneous resolution thresholds like the "gold standard FSC 0.143" (based on "sloppy statistics" see our bioRxiv paper)" - it makes it impossible to objectively compare the resolution levels achieved in independent experiments. That makes efforts to perform projects like "resolution challenges" futile and not worth wasting your time on.<br>
<br>
Cheers,<br>
Marin<br>
<br>
===============<br>
<br>
On 02/09/2018 07:14, Sjors Scheres wrote:<br>
<br>
<br>
Dear Natesh,<br>
The limitation of 3x the pixel size of the maximum reachable resolution might be true for software written by Marin, but it is definitely not true for many other programs, including relion. We routinely achieve resolutions very close to Nyquist, which is only 2x the pixel size.<br>
<br>
Hth,<br>
Sjors<br>
Sent from my phone.<br>
<br>
<br>
-------- Original message --------<br>
From: Marin van Heel <<a href="mailto:marin.vanheel@googlemail.com" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">marin.vanheel@googlemail.com</a>><br>
Date: 01/09/2018 19:43 (GMT+01:00)<br>
To: Natesh Ramanathan <<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a>>, <a href="mailto:3dem@ncmir.ucsd.edu" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">3dem@ncmir.ucsd.edu</a><br>
Subject: Re: [3dem] GroEL best resolution map. (FSC RESOLUTION ??)<br>
<br>
<br>
Dear Natesh,<br>
<br>
I just checked the technical data of the EMD-3407 dataset and I saw that its pixel size is 1.05A . That means that no resolution levels better than 3 x 1.05A = 3.15A may be claimed for processing results based on that data set, following the standards we set in the past (for the younger generation: I am the main inventor of the FSC ;) ). Going beyond a resolution claim of 3.15A would mean that the dataset is undersampled, and would thus require a new data collection with a smaller pixel size. For a possible resolution claim of, say, 2A you need a maximum pixel size of 0.66A ! Moreover, the 0.143 threshold FSC threshold used in this EMD-3407 case is itself wrong, while based on sloppy statistics. (<a href="https://www.biorxiv.org/content/early/2017/11/24/224402).Bottom" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">https://www.biorxiv.org/content/early/2017/11/24/224402).Bottom</a> line: to compare the results of different cryo-EM experiments you need correct resolution criteria applied to sufficiently<br>
fine sampled cryo-EM data.<br>
<br>
Cheers<br>
<br>
Marin<br>
<br>
On 01/09/2018 12:57, Natesh Ramanathan wrote:<br>
<br>
Dear All,<br>
<br>
Does anyone know/have a GroEL cryoEM map with resolution 2.5 ? or better?<br>
I see that the best resolution in EMDataBank is 3.26 ? - EMD-3407 and EMD-3415.<br>
<br>
<br>
If you have one, is it possible to share the map with me? <br>
<br>
Many thanks.<br>
Best regards,<br>
Natesh<br>
--<br>
<br>
----------------------------------------------------------<br>
"Live Simply and do Serious Things .. "<br>
- Dorothy Mary Crowfoot Hodgkin OM, FRS<br>
<br>
"In Science truth always wins"<br>
- Max Ferdinand Perutz OM FRS<br>
----------------------------------------------------------<br>
Dr. Ramanathan Natesh<br>
Assistant Professor, <br>
School of Biology,<br>
Indian Institute of Science Education and Research Thiruvananthapuram (IISER-TVM),<br>
<br>
Maruthamala P.O., Vithura,<br>
<br>
Thiruvananthapuram, 695551, Kerala, India<br>
<br>
<a href="mailto:natesh@iisertvm.ac.in" rel="noreferrer noreferrer noreferrer noreferrer" target="_blank">natesh@iisertvm.ac.in</a><br>
<a href="http://www.researcherid.com/rid/C-4488-2008" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">http://www.researcherid.com/rid/C-4488-2008</a><br>
ORCID: <a href="http://orcid.org/0000-0002-1145-5962" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">http://orcid.org/0000-0002-1145-5962</a><br>
<a href="https://publons.com/author/1520837/ramanathan-natesh#profile" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">https://publons.com/author/1520837/ramanathan-natesh#profile</a><br>
<a href="http://faculty.iisertvm.ac.in/natesh" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">http://faculty.iisertvm.ac.in/natesh</a><br>
<br>
Office Ph. 0091- 471-2778087<br>
<br>
<br>
<br>
<br>
_______________________________________________ 3dem mailing list3dem@ncmir.ucsd.eduhttps://<a href="http://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>
<br>
<br>
-- ============================================================== Prof Dr Ir Marin van Heel Laborat?rio Nacional de Nanotecnologia - LNNano CNPEM/LNNano, Campinas, Brazil tel: +55-19-3518-2316 mobile +55-19-983455450 (current) mobile +55-19-981809332 (041-19-981809332 TIM) Skype: Marin.van.Heel email: marin.vanheel(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a> marin.vanheel(A_T)<a href="http://lnnano.cnpem.br" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">lnnano.cnpem.br</a> and: mvh.office(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a> -------------------------------------------------- Emeritus Professor of Cryo-EM Data Processing Leiden University Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS) -------------------------------------------------- Emeritus Professor of Structural Biology Imperial College London Faculty of Natural Sciences email: m.vanheel(A_T)<a href="http://imperial.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">imperial.ac.uk</a> -------------------------------------------------- I<br>
receive many emails per day and, although I try, there is no guarantee that I will actually read each incoming email. <br>
<br>
<br>
-- ============================================================== Prof Dr Ir Marin van Heel Laborat?rio Nacional de Nanotecnologia - LNNano CNPEM/LNNano, Campinas, Brazil tel: +55-19-3518-2316 mobile +55-19-983455450 (current) mobile +55-19-981809332 (041-19-981809332 TIM) Skype: Marin.van.Heel email: marin.vanheel(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a> marin.vanheel(A_T)<a href="http://lnnano.cnpem.br" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">lnnano.cnpem.br</a> and: mvh.office(A_T)<a href="http://gmail.com" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">gmail.com</a> -------------------------------------------------- Emeritus Professor of Cryo-EM Data Processing Leiden University Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS) -------------------------------------------------- Emeritus Professor of Structural Biology Imperial College London Faculty of Natural Sciences email: m.vanheel(A_T)<a href="http://imperial.ac.uk" rel="noreferrer noreferrer noreferrer noreferrer noreferrer" target="_blank">imperial.ac.uk</a> -------------------------------------------------- I<br>
receive many emails per day and, although I try, there is no guarantee that I will actually read each incoming email. <br>
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End of 3dem Digest, Vol 133, Issue 4<br>
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