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<div class="">National Resource for Automated Molecular Microscopy (NRAMM)</div>
<div class="">and</div>
<div class="">Netherlands Centre for Electron Nanoscopy (NeCEN)</div>
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<div class="">A Workshop on Challenges for High Speed Cryo Electron Tomography</div>
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<div class="">28-29 November, 2018 </div>
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<div class="">National Resource for Automated Molecular Microscopy</div>
<div class="">Simons Electron Microscopy Center</div>
<div class="">New York Structural Biology Center, New York NY</div>
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<div class="">Cryo electron tomography (cryoET) is an enabling technique for structural studies of molecular machines either in vitro or in situ. During the last five years the performance in terms of resolution and applicability cryoET has been enormous due
to the game changing technologies that transformed the application of cryoEM in general. The emergence of highly sensitive and faster detectors with electron counting capability, improved properties for prolonged and automated cryoEM data collection sessions,
and optimized software and hardware for single particle analysis has resulted in mature and reasonably high throughput workflows for cryoEM. In contrast to the cryoEM workflow, existing cryoET workflows are low throughput.</div>
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<div class="">We invite applications to participate in a workshop to discuss the challenges and opportunities for high speed cryoET. We will focus on understanding current workflows for cryoET, identifying the most critical bottlenecks related to quality and
throughput, and proposing future work to address these bottlenecks. Aspects to be discussed will include: (i) Goniometers: eccentricity, stability, speed, repeatability, continuous tilting; (ii) Automation; (iii) Cameras: delivery and recovery speed, acquisition
modes; (iii) Novel strategies: dose rate, step size; phase plates; (iv) Image formation of highly tilted specimens; (v) Preprocessing; (vi) Need for dedicated hardware and software.</div>
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<div class="">Apply to participate at: <a href="https://www.surveymonkey.com/r/NZ6GLZT" class="">
https://www.surveymonkey.com/r/NZ6GLZT</a></div>
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<div class="">The application deadline is 30 September and a final selection of participants will be made by 15 October. The workshop will be limited to 25 participants.</div>
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<div class="">For any other enquiries please send email to: <a href="mailto:nramm@nysbc.org" class="">
nramm@nysbc.org</a></div>
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<div class="">Organizers:</div>
<div class="">Bridget Carragher and Clint Potter, National Resource for Automated Molecular Microscopy (NRAMM)</div>
<div class="">Bram Koster, Netherlands Centre for Electron Nanoscopy (NeCEN)</div>
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<div class="">Clint Potter</div>
<div class="">NRAMM: National Resource for Automated Molecular Microscopy</div>
<div class="">NCCAT: National Center for CryoEM Access and Training</div>
<div class="">Simons Electron Microscopy Center</div>
<div class="">New York Structural Biology Center</div>
<div class="">89 Convent Ave., New York, NY 10027</div>
<div class="">(212) 939-0660 x301</div>
<div class=""><a href="mailto:cpotter@nysbc.org" class="">cpotter@nysbc.org</a></div>
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