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Dear Colleagues
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<div class="">Louise Fairall and the Protex facility here at Leicester have made a construct of Human H chain Ferritin with a TEV cleavable GST tag. We (and others) have used protein expressed from this plasmid for purposes of testing our microscopes and have
obtained maps in the 2.1Å range. Its also handy for training purposes and teaching newcomers how to make good grids. If anybody would like a few microliters of this plasmid we will be happy to provide some. Please email me and we can arrange a way to send
it to you along with the expression and purification details. Purification is straight forward involving binding to GST resin, TEV cleavage and an S-200 SEC column. </div>
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<div class="">Best wishes</div>
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<p class="MsoNormal" style="font-weight: normal; margin-bottom: 12pt;"><b class=""><span style="font-size: 9pt; color: rgb(31, 73, 125);" class="">Dr Christos Savva</span></b><span style="font-size: 9pt; font-family: Helvetica; color: black;" class=""><o:p class=""></o:p></span></p>
<p class="MsoNormal"><span class="" style="font-size: 9pt; font-family: Helvetica; color: rgb(0, 112, 192);"><a href="http://www.leicester.ac.uk/cryoem" class=""><span class="" style="color: rgb(0, 112, 192); text-decoration: none;"><b class="">Midlands Regional
Cryo-EM Facility</b></span></a></span><span class="" style="font-weight: normal; font-family: Helvetica; color: rgb(0, 112, 192);"><o:p class=""></o:p></span></p>
<p class="MsoNormal" style="font-weight: normal;"><span style="font-size: 9pt; font-family: Helvetica; color: rgb(0, 84, 147);" class="">University of Leicester</span><span style="font-size: 11pt; color: rgb(0, 84, 147);" class=""><br class="">
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</span><span style="font-size: 9pt; font-family: Helvetica; color: rgb(0, 84, 147);" class="">Leicester </span><span style="font-size: 11pt; color: rgb(0, 84, 147);" class=""><br class="">
</span><span style="font-size: 9pt; font-family: Helvetica; color: rgb(0, 84, 147);" class="">LE1 7HB</span></p>
<p class="MsoNormal" style="font-weight: normal;"><b class=""><span style="font-size: 10pt; font-family: Helvetica; color: rgb(187, 0, 0);" class="">t:</span></b><span style="font-size: 10pt; font-family: Helvetica; color: black;" class=""> +44 116 229 7109<br class="">
</span><b class=""><span style="font-size: 10pt; font-family: Helvetica; color: rgb(187, 0, 0);" class="">e:</span></b><span style="font-size: 10pt; font-family: Helvetica; color: black;" class=""> </span><span style="font-size: 10pt; font-family: "Times New Roman", serif;" class=""><a href="mailto:pjw17@le.ac.uk" target="_blank" class=""><span style="font-family: Helvetica;" class="">gwcs1@le.ac.uk</span></a></span><span style="font-size: 10pt; font-family: Helvetica; color: black;" class=""><br class="">
</span><b class=""><span style="font-size: 10pt; font-family: Helvetica; color: rgb(187, 0, 0);" class="">w:</span></b><u class=""><span style="font-size: 10pt; font-family: Helvetica; color: blue;" class=""><a href="http://www.le.ac.uk/cryoem" target="_blank" class="">www.le.ac.uk/cryoem</a></span></u><span style="font-family: Helvetica; color: black;" class=""><br class="">
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