<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:PMingLiU;
panose-1:2 2 5 0 0 0 0 0 0 0;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"\@PMingLiU";
panose-1:2 2 5 0 0 0 0 0 0 0;}
@font-face
{font-family:"Comic Sans MS";
panose-1:3 15 7 2 3 3 2 2 2 4;}
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri",sans-serif;
mso-fareast-language:EN-US;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
{mso-style-priority:99;
mso-style-link:"Balloon Text Char";
margin:0in;
margin-bottom:.0001pt;
font-size:8.0pt;
font-family:"Tahoma",sans-serif;
mso-fareast-language:EN-US;}
p.msonormal0, li.msonormal0, div.msonormal0
{mso-style-name:msonormal;
mso-margin-top-alt:auto;
margin-right:0in;
mso-margin-bottom-alt:auto;
margin-left:0in;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
span.BalloonTextChar
{mso-style-name:"Balloon Text Char";
mso-style-priority:99;
mso-style-link:"Balloon Text";
font-family:"Tahoma",sans-serif;}
span.EmailStyle20
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle21
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="blue" vlink="purple">
<div class="WordSection1">
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Hi Eric,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">We don’t see those gold particles (or only very few) that may come off the UltrAuFoils when using our Gatan Solarus. We use a hydrogen/oxygen mix for plasma cleaning and get excellent wetting for
both carbon and gold foils. We get better spreading with hydrogen than argon.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Our settings are <o:p>
</o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">O2 gas flow 27.5 sccm<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">H2 gas flow 6.4 sccm<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Forward RF target 20W<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Forward RF Range 5W<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Maximum Reflected RF 5W<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Vacuum target 70 mTorr<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Cleaning time is typically 30 sec for Quantifoils and 2 min for UltrAuFoils under these conditions.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW">Best regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW"> Matthias<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:ZH-TW"><o:p> </o:p></span></p>
<div style="border:none;border-left:solid blue 1.5pt;padding:0in 0in 0in 4.0pt">
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span style="mso-fareast-language:ZH-TW">From:</span></b><span style="mso-fareast-language:ZH-TW"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Eric Hanssen<br>
<b>Sent:</b> Wednesday, July 4, 2018 11:45 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu; CCPEM@JISCMAIL.AC.UK<br>
<b>Subject:</b> [3dem] ultrAufoil<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-AU">Hi all, <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">We have been using UltrAufoil for a few weeks now with great success in terms of stability and reproducibility. However also reproducible are gold particles in the holes (see picture of an extreme case). These are certainly
great for tomography not so much for SPA. <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Anyway is there a specific way to clean these grids in order to get rid of the unwanted little buggers?<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">We usually glow discharge our grids with the Pelco easyglow system and its default settings in air, one side only.
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Regards<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Eric<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:navy;mso-fareast-language:EN-AU">-----------------<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:navy;mso-fareast-language:EN-AU">Assoc. Prof Eric Hanssen<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:navy;mso-fareast-language:EN-AU"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><b><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:#1F497D;mso-fareast-language:EN-AU">Head - Advanced Microscopy Facility
<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:#1F497D;mso-fareast-language:EN-AU">Honorary Principal Fellow – Department of Biochemistry and Molecular Biology<o:p></o:p></span></b></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:#1F497D;mso-fareast-language:EN-AU"><br>
Bio21 Molecular Science and Biotechnology Institute<br>
</span><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:gray;mso-fareast-language:EN-AU">30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia<br>
email: </span><span lang="EN-AU"><a href="mailto:ehanssen@unimelb.edu.au"><span style="font-size:10.0pt;font-family:"Comic Sans MS";color:gray;mso-fareast-language:EN-AU">ehanssen@unimelb.edu.au</span></a></span><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:gray;mso-fareast-language:EN-AU">
| Office: +61 3 83442449 | Microscope: +61 3 83442509 <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:gray;mso-fareast-language:EN-AU">Web:
</span><span lang="EN-AU"><a href="http://www.microscopy.unimelb.edu.au/"><span style="font-size:10.0pt;font-family:"Comic Sans MS";mso-fareast-language:EN-AU">www.microscopy.unimelb.edu.au</span></a></span><span lang="EN-AU" style="font-size:10.0pt;font-family:"Comic Sans MS";color:gray;mso-fareast-language:EN-AU"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU"><img border="0" width="624" height="290" style="width:6.5in;height:3.0208in" id="Picture_x0020_1" src="cid:image001.png@01D4138E.64BC41D0" alt="qqwd"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
</div>
</div>
</body>
</html>