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<p class="MsoNormal"><span style="color:#1F497D">Hi Jillian and Reza,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">This paper should have some useful answers for you;
<a href="https://www.ncbi.nlm.nih.gov/pubmed/29395788">https://www.ncbi.nlm.nih.gov/pubmed/29395788</a>. It introduces a method to calculate local resolution “MonoRes” by J. Vilas et al (Structure, 2018). They propose a fully automatic and accurate method to
determine the local resolution of 3D map. And, the method is available in the Scipion.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#333333;background:white">Cheers,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#333333;background:white">Mohsen</span><span style="color:#1F497D"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222;background:white">-- </span><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">Mohsen Kazemi, PhD, PEng</span></b><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:blue">Postdoctoral Research Fellow</span><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">Department of Biochemistry and Molecular Biology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">School of Biomedical Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">Faculty of Medicine, Dentistry and Health Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">The University of Melbourne<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">Room 363,</span><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">
</span><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">David Penington Building (Bio21 Institute), 30 Flemington Rd, Parkville, VIC 3052, Australia<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">Phone : +61 3 834 40285<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">Email : <a href="mailto:mohsen.kazemi@unimelb.edu.au" target="_blank"><span style="color:#1155CC">mohsen.kazemi@unimelb.edu.au</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Times New Roman",serif;color:#222222">
<a href="mailto:mohsenkazemi20@gmail.com" target="_blank"><span style="color:#1155CC">mohsenkazemi20@gmail.com</span></a> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> <b>On Behalf Of
</b>Jillian Chase<br>
<b>Sent:</b> Tuesday, June 12, 2018 7:55 AM<br>
<b>To:</b> Reza Khayat <rkhayat@ccny.cuny.edu><br>
<b>Cc:</b> 3dem@ncmir.ucsd.edu; CCPEM@JISCMAIL.AC.UK<br>
<b>Subject:</b> Re: [3dem] Local resolution image<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Hi All,<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I'm also very curious about this- has any standard been implemented? It would be nice to have a general guideline so reported data is more-or-less equally easy to interpret in various papers. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We are in the process of drafting a manuscript and have gone back and forth about how to show local resolution in an intuitive way. Some areas of our map are better or worse than the global resolution. What we've settled on (for now) is
a rainbow color spectrum (Chimera) in which the range spans both above and below our global res and we have included the scale next to our map colored by res and labeled with resolution. Is there a universal standard that the community is moving towards for
this or are we approaching it correctly (or as best we can) for now?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Any insight would be very useful. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">All the best,<o:p></o:p></p>
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<p class="MsoNormal">Jillian<o:p></o:p></p>
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<p class="MsoNormal"><br>
Jillian Chase <o:p></o:p></p>
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<p class="MsoNormal">PhD Candidate, Jeruzalmi Lab<o:p></o:p></p>
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<p class="MsoNormal">CUNY/CCNY<o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><br>
On Jun 11, 2018, at 4:58 PM, Reza Khayat <<a href="mailto:rkhayat@ccny.cuny.edu">rkhayat@ccny.cuny.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">Hi,<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Has a standard been established in the field for coloring local resolution maps? If not, are there any suggestions (i.e. FSC resolution is blue, 2x FSC resolution is white, and 4x FSC resolution is red). Thanks.<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Best wishes,<o:p></o:p></p>
<p class="MsoNormal">Reza<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Reza Khayat, PhD</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Assistant Professor</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Department of Chemistry</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">City College of New York</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">85 Saint Nicholas Terrace, CDI 2.318</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">New York, NY 10031</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D"><a href="http://www.khayatlab.org/">http://www.khayatlab.org/</a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">212-650-6070</span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Times New Roman",serif">_______________________________________________<br>
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