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Dear colleagues,
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<div class="">I’d lke to draw your attention to the 3D-FSC server that we’ve put together for use among the cryo-EM community: </div>
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<div class=""><a href="https://3dfsc.salk.edu" class="">https://3dfsc.salk.edu</a></div>
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<div class="">The 3D-FSC provides a quantitative measure of directional resolution anisotropy, which is caused by preferential orientation at the air-water interface, as described in <a href="https://www.ncbi.nlm.nih.gov/pubmed/28671674" class="">Tan et al
2017</a> We have consistently found that in many single-particle experiments, especially in cases with severe preferential orientation, the global FSC alone is insufficient to accurately gauge the quality of a dataset, and in some cases, can even bely the
interpretability of the map. Thus, we feel that this is an important quality control measure for single-particle reconstructions in general, and we hope that the community will find it useful for evaluating their density maps. </div>
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<div class="">Regards, </div>
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<div class="">Dmitry</div>
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