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Hi,
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<div class="">We have had a really good experience with our Falcon 3. In Leeds we have two Krioses, one with F3 and the second with energy filtered K2 and phase plate. The filtered K2 is undeniably better for tomography, but for a broad range of single particle
applications we have been really impressed with the F3. We offer external access to academics and industry, for these users for single particle applications usage is split pretty evenly across the K2 and F3. Our internal users for standard single particle
applications probably lean towards the F3.</div>
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<div class="">In counting mode at the moment we usually get ~ 25 images/hour, and we have got a sub 3 A structures routinely for specimens we would expect to get there, but interestingly a lot of the time we are using the detector in integrating mode! For larger
specimens (viruses/ribosomes) we routinely collect on Quantifoil grids and get 70-90 images/hour, or carbon backed lacey grids where we can get over 150 images an hour. The speed of collection means we can routinley get to sub 3 A with relatively short data
collection sessions (especially with icosahedral viruses!). But we have also found the F3 in integrating mode ideal for looking at more heterogeneous and flexible specimens (benefit from larger datasets over a comparable time period compared with counting,
datasets which can then be sub classified) even when these are relatively small (for example see Ieva Drulyte’s <a href="https://twitter.com/IevaDrulyte/status/960917363930562560" class="">tweet</a>). We compensate for the loss of contrast compared with counting
mode by using relatively high doses (60-110 e/A2). We don’t have a phase plate on our Falcon 3 microscope so I can’t comment on this. </div>
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<div class="">Hope this helps!</div>
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<div class="">Best wishes,</div>
<div class="">Becky</div>
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Rebecca Thompson<br class="">
Senior cryo-Electron Microscopy Support Scientist/Facility manager <br class="">
Astbury Biostructure Laboratory<br class="">
University of Leeds</div>
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Email<span class="Apple-tab-span" style="white-space: pre;"> </span><a href="mailto:r.f.thompson@leeds.ac.uk" class="">r.f.thompson@leeds.ac.uk</a><br class="">
Phone<span class="Apple-tab-span" style="white-space: pre;"> </span>0113 3438957/3438959 (office/Titan Krios control room)</div>
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Mobile<span class="Apple-tab-span" style="white-space: pre;"> </span>07816179813<br class="">
Location<span class="Apple-tab-span" style="white-space: pre;"> </span>Roger Stevens 5.40<br class="">
Linkedin<span class="Apple-tab-span" style="white-space: pre;"> </span> <span class="Apple-tab-span" style="white-space: pre;">
</span><a href="https://uk.linkedin.com/in/1rebeccathompson" class="">https://uk.linkedin.com/in/1rebeccathompson</a><br class="">
Twitter<span class="Apple-tab-span" style="white-space: pre;"> </span> Bex_16</div>
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<blockquote type="cite" class="">
<div class="">On 31 May 2018, at 01:53, Masahide Kikkawa <<a href="mailto:mkikkawa@gmail.com" class="">mkikkawa@gmail.com</a>> wrote:</div>
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<div class="">Hi,</div>
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</div>
<div class=""> I guess it also depends on your application. </div>
<div class="">Our recent test shows that Falcon 3 is excellent for single particle analysis:</div>
<div class="">See Rado's tweet:</div>
<div class=""><a href="https://twitter.com/RadoDanev/status/964551530878775297" class="">https://twitter.com/RadoDanev/status/964551530878775297</a></div>
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<div class="">---------------------------------------------------------------<br class="">
Masahide Kikkawa, M.D., Ph. D.<br class="">
Professor<br class="">
Department of Cell Biology & Anatomy<br class="">
Graduate School of Medicine<br class="">
The University of Tokyo<br class="">
<a href="http://structure.m.u-tokyo.ac.jp/" class="">http://structure.m.u-tokyo.ac.jp/</a><br class="">
tel: +81-3-5841-3338<br class="">
fax: +81-3-5841-3339<br class="">
---------------------------------------------------------------<br class="">
<br class="">
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<br class="">
<blockquote type="cite" class="">
<div class="">
<div class="">Hi,<br class="">
<br class="">
What is the EM communities feelings and experience with the Falcon 3 cameras? We are in the midst of an instrument purchase and have been leaning towards a Falcon 3, but do have a small window of opportunity to switch to other cameras if there are good
reasons. Please let me know your experiences. Thanks.<br class="">
<br class="">
--<br class="">
politics is more difficult than physics.<br class="">
A. Einstein<br class="">
<br class="">
David Gene Morgan<br class="">
Electron Microscopy Center<br class="">
047D Simon Hall<br class="">
IU Bloomington<br class="">
812 856 1457 (office)<br class="">
812 856 3221 (3200)<br class="">
<a href="http://iubemcenter.indiana.edu/" class="">http://iubemcenter.indiana.edu</a><br class="">
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