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<div class="gmail_quote">Third Announcement<br>
<br>
THE EIGHTH BRAZIL SCHOOL FOR SINGLE PARTICLE CRYO-EM.<br>
<br>
27 Sept - 9 Oct 2018, Mairiporã, SP Brazil (<a
href="http://www.brazil-school.org" rel="noreferrer"
target="_blank" moz-do-not-send="true">www.brazil-school.org</a>)<br>
<br>
Our single-particle school is a unique and intensive 12-day
hands-on course/workshop, in which theory and practice are
tightly intertwined. The understanding of the principles of
cryo-EM data processing stands central in this event and a few
cryo-EM pioneers will explain the approaches to the
participants to a level where they can apply it themselves.
The spectrum of topics covered suits attendees who are new to
the field as well as those for whom the technique is of
growing importance and who wish to acquire an in-depth
understanding of the fundamental principles. The final days
of the meeting will be devoted to advanced topics such as
high-resolution refinements and the processing of
heterogeneous data sets ("4D cryo–EM”). Advanced topics will
include:<br>
<br>
1) Optimizing data collection with direct electrons detectors
and exploiting movies.<br>
2) Full data set automatic CTF correction.<br>
3) Correction of anisotropic magnification.<br>
4) MSA data compression + unsupervised classification, from
the very inventors of 2D- and 3D-classification.<br>
5) Reaching atomic resolution without bias:
alignment-by-classification (ABC-4D).<br>
6) Unbiased "Random Startup" of 3D and 4D reconstructions.<br>
7) Projection Matching and Angular Reconstitution: Pros
&Cons.<br>
8) 4D refinement procedures for high-resolution results using
different software systems: Frealign/cisTEM, cryoSPARC,
Relion, IMAGIC, etc.<br>
9) Fitting and refining atomic coordinates in high-resolution
cryo-EM maps using programs such as REFMAC and Coot.<br>
10) Validation and interpretation of final results using
reproducible (local) resolution metrics.<br>
<br>
Kind regards,<br>
<br>
Marin van Heel<br>
<br>
Note: Registrations will remain open until June 10th, because
of the forthcoming long holiday weekend. The results of the
final selection will be announced by Monday the 18th of June.<br>
<br>
==============================<wbr>==============================<wbr>==<br>
<br>
Prof Dr Ir Marin van Heel<br>
<br>
Laboratório Nacional de Nanotecnologia - LNNano<br>
CNPEM/LNNano, Campinas, Brazil<br>
<br>
------------------------------<wbr>--------------------<br>
<br>
I receive many emails per day and, although I try,<br>
there is no guarantee that I will actually read each incoming
email.<br>
<br>
<br>
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