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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Dear Toby,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">We’re also using the system and are very happy. We have just received a bespoke version designed to hold HPF samples which is looking to work well so far. <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Happy to answer any questions
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Best<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Roland
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Professor Roland A. Fleck FRCPath, FRMS<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Director, Centre for Ultrastructural Imaging<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">hcpc Clinical Scientist<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><a href="mailto:roland.fleck@kcl.ac.uk" title="mailto:roland.fleck@kcl.ac.uk
Ctrl+Click or tap to follow the link"><span style="color:#0563C1">roland.fleck@kcl.ac.uk</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">King’s College London<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">New Hunts House<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Guy's Campus<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">London SE1 1UL<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Tel/Fax 020 7848 6950<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">https://cui.kcl.ac.uk</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Visiting Professor Faculty of Health and Medical Sciences
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">University of Copenhagen<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="color:black">From: </span></b><span style="color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Mykhailo Kopylov <mn12@my.fsu.edu><br>
<b>Date: </b>Friday, 25 May 2018 at 16:20<br>
<b>To: </b>Reza Khayat <rkhayat@ccny.cuny.edu>, Toby Starborg <tobias.starborg@manchester.ac.uk>, "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] Any SubAngstrom storage system users?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Dear Toby,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">We have been using SubAngstrom pucks/cane system at NYSBC-SEMC for quite a while, and we are very satisfied with the switch from the conical tubes. The system is great as a
storage, but more importantly, it also made it much easier for us to organize and track grid boxes.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Let me know if you have any specific questions – I would be happy to answer.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Sincerely,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Misha</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> --</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Calibri",sans-serif">Mykhailo (Misha) Kopylov, Ph.D.<br>
Scientist, Simons Electron Microscopy Center<br>
New York Structural Biology Center<br>
89 Convent Ave, New York, NY 10027</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Calibri",sans-serif"><a href="https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsemc.nysbc.org%2F&data=01%7C01%7Croland.fleck%40kcl.ac.uk%7Cfa8d1cbcb39b4e75035108d5c25301f3%7C8370cf1416f34c16b83c724071654356%7C0&sdata=7AYBY47K0VKN%2Ffb7jEzRNJnl7LQpKKZv5tVYBpaXwug%3D&reserved=0"><span style="color:#0563C1">semc.nysbc.org</span></a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Calibri",sans-serif">(212) 939-0660 <br>
<a href="mailto:mkopylov@nysbc.org"><span style="color:#0563C1">mkopylov@nysbc.org</span></a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> </span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="color:black">From: </span></b><span style="color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Reza Khayat <rkhayat@ccny.cuny.edu><br>
<b>Date: </b>Friday, May 25, 2018 at 10:58 AM<br>
<b>To: </b>Toby Starborg <tobias.starborg@manchester.ac.uk>, "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] Any SubAngstrom storage system users?</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Hi Toby,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">We have the SubAngstrom storage system and are quite happy with it. Cleaned up the dewar and got rid of all those fishing lines.
</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Best wishes,<br>
Reza</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"> </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Reza Khayat, PhD</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Assistant Professor</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Department of Chemistry</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">City College of New York</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">85 Saint Nicholas Terrace, CDI 2.318</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">New York, NY 10031</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><a href="https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.khayatlab.org%2F&data=01%7C01%7Croland.fleck%40kcl.ac.uk%7Cfa8d1cbcb39b4e75035108d5c25301f3%7C8370cf1416f34c16b83c724071654356%7C0&sdata=ZKM76k6JCdki4r9M%2F0ocUIfsLQlPqcNacVIE%2BtQGiUM%3D&reserved=0"><span style="color:#0563C1">http://www.khayatlab.org/</span></a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">212-650-6070</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"> </span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Mariena Silvestry Ramos<br>
<b>Sent:</b> Friday, May 25, 2018 10:55 AM<br>
<b>To:</b> Toby Starborg <tobias.starborg@manchester.ac.uk>; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] Any SubAngstrom storage system users?</span><o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p><span style="color:black">Hi Toby, </span><o:p></o:p></p>
<p><span style="color:black"> </span><o:p></o:p></p>
<p><span style="color:black">We have at least one user at our facility who's purchased the system. I like that there's more room/space to maneuver than if you have a conical tube with 10 grid boxes and have a user who labels everything in the faintest shade
of blue and makes the box unreadable to my aging eyes. It can sometimes be a wee painful to fish out the box you want when you're under LN2, but once your eyes adjust to having both the LN2 and the plastic lid there, you can generally move things around is
some ease which I do with the big tweezers. I second the opinion that if you have access to a machine shop, have them make a prototype and test it. In the long run it may be more cost-effective and/or easier to modify if/as needed. </span><o:p></o:p></p>
<p><span style="color:black"> </span><o:p></o:p></p>
<p><span style="color:black">Best, </span><o:p></o:p></p>
<p><span style="color:black"> </span><o:p></o:p></p>
<p><span style="color:black">Mariena </span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>>
on behalf of Toby Starborg <<a href="mailto:tobias.starborg@manchester.ac.uk">tobias.starborg@manchester.ac.uk</a>><br>
<b>Sent:</b> Friday, May 25, 2018 5:52:39 AM<br>
<b>To:</b> <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<b>Subject:</b> [3dem] Any SubAngstrom storage system users?</span> <o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Dear cryo-EM users,<br>
<br>
I'm wondering if anyone has experience using the SubAngstrom grid <br>
storage system? I'm looking for both positive and negative feedback, <br>
but if you don't want to post directly to the list, feel free to email <br>
me directly.<br>
<br>
<br>
Thank you for any information that you may have.<br>
<br>
<br>
Toby S<br>
<br>
<br>
(I don't know whether I'm allowed to post links to company websites as <br>
it may be seen as advertising, but the system is seen here: <br>
<a href="https://emea01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__subangstrom.com_%26d%3DDwMFAg%26c%3D4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo%26r%3D1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0%26m%3DzKn7Juqz9LBVG_d3AUz5CCfltmXxdGez723ceBZTDgA%26s%3DzMX52K4YPrwmi34j2x_aicrcR2XQ8pznGNPVthxutsA%26e%3D&data=01%7C01%7Croland.fleck%40kcl.ac.uk%7Cfa8d1cbcb39b4e75035108d5c25301f3%7C8370cf1416f34c16b83c724071654356%7C0&sdata=Wtw2FxsjBnHUzn1dxpg0oSJudBDhHfDcvG9Ymqqojuc%3D&reserved=0">https://subangstrom.com/</a>).<br>
<br>
-- <br>
Dr Tobias Starborg<br>
Senior Experimental Officer: 3DEM<br>
Wellcome Centre for Cell Matrix Research<br>
Michael Smith Building<br>
Manchester<br>
M13 9PT<br>
Tel: +44(0)1612755170<br>
<a href="https://emea01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__www.polara.manchester.ac.uk%26d%3DDwMFAg%26c%3D4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo%26r%3D1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0%26m%3DzKn7Juqz9LBVG_d3AUz5CCfltmXxdGez723ceBZTDgA%26s%3D9zQZVZEIoUvHLAndJ7LTNrcdRuEqD1pzf-Ba79ryvZE%26e%3D&data=01%7C01%7Croland.fleck%40kcl.ac.uk%7Cfa8d1cbcb39b4e75035108d5c25301f3%7C8370cf1416f34c16b83c724071654356%7C0&sdata=F%2F3xM6PyQ2TgPATO%2BSV%2B1Mt9pJf5KtEWYl2l0ePrFcY%3D&reserved=0">http://www.polara.manchester.ac.uk</a><br>
<br>
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<a href="https://emea01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__mail.ncmir.ucsd.edu_mailman_listinfo_3dem%26d%3DDwMFAg%26c%3D4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo%26r%3D1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0%26m%3DzKn7Juqz9LBVG_d3AUz5CCfltmXxdGez723ceBZTDgA%26s%3Dk4ZvHkPsJmfq1Q0S10jZYNz6PzQhi-mqx8CO7W8W23g%26e%3D&data=01%7C01%7Croland.fleck%40kcl.ac.uk%7Cfa8d1cbcb39b4e75035108d5c25301f3%7C8370cf1416f34c16b83c724071654356%7C0&sdata=5fVuMirBD1vhib3cN3qjIZaAyjG886N0n4fISuRjVmI%3D&reserved=0">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></span><o:p></o:p></p>
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