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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Michaela James | HR Advisor – Monday, Tuesday & Friday | Victor Chang Cardiac Research Institute | 405 Liverpool St, Darlinghurst NSW 2010 Australia | P: 02
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Montserrat Samso<br>
<b>Sent:</b> Tuesday, 24 April 2018 4:19 AM<br>
<b>To:</b> Bowman, Valorie D; Artemis Kosta; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] [EXTERNAL] Re: Vitrobot humidifier problem<o:p></o:p></span></p>
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<p><span style="font-family:"Calibri","sans-serif";color:black">Dear All,<o:p></o:p></span></p>
<p><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p><span style="font-family:"Calibri","sans-serif";color:black">We had the same problem with our Vitrobot Mk 4. We disassembled the humidifier connector to the back of the machine and found some loose connections. After re-assembling properly this connection,
the humidification worked fine again!<o:p></o:p></span></p>
<p><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:black">So it is worth a try.
<o:p></o:p></span></p>
<p><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p><span style="font-family:"Calibri","sans-serif";color:black">Good luck,<o:p></o:p></span></p>
<p><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Montserrat Samso, Ph.D.<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Assistant Professor<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Director, CryoEM Facility<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Dept. Physiology and Biophysics<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Virginia Commonwealth University<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">1101 E. Marshall St., RM 3-009<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Richmond, VA 23298<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Tel: (804) 828-8728<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Fax: (804) 828-8492<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">E-mail:
<a href="mailto:Montserrat.Samso@vcuhealth.org">Montserrat.Samso@vcuhealth.org</a><o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Bowman, Valorie
D <vdb@purdue.edu><br>
<b>Sent:</b> Monday, April 23, 2018 2:05:49 PM<br>
<b>To:</b> Artemis Kosta; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [EXTERNAL] Re: [3dem] Vitrobot humidifier problem</span><span style="font-family:"Calibri","sans-serif";color:black">
<o:p></o:p></span></p>
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<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Dear All,<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">We recently have started having trouble getting our old Vitrobot humidifier to reach a high humidity when it's very dry in the lab. It doesn't give any error messages
like you've seen, it just doesn't go up. I resolved it by using very hot water like we do for the CP3 sponge. Not a very elegant solution, but it works!<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">David's sponge idea is a good one too if the little unit is completely kaput.<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">Cheers,</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><i><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">Valorie</span></i><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><i><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black">Valorie Bowman</span></i><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
<br>
<a href="mailto:vdb@purdue.edu">vdb@purdue.edu</a><br>
765-494-5643<br>
EM Facility Laboratory Manager/<br>
Senior Research Electron Microscopist<br>
Purdue Cryo EM Facility<br>
Purdue University<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> 3dem <3dem-bounces@ncmir.ucsd.edu>
on behalf of Morgan, David Gene <dagmorga@indiana.edu><br>
<b>Sent:</b> Monday, April 23, 2018 11:09 AM<br>
<b>To:</b> Artemis Kosta; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] Vitrobot humidifier problem</span><span style="font-family:"Calibri","sans-serif";color:black">
<o:p></o:p></span></p>
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<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black">Hi,<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"> We had a similar problem with a Mk 3. After multiple attempts to fix various thing and some conversations with FEI, we eventually needed to have service come and fix
the problem. As I remember (this was several years ago), it required a new circuit board of some sort. Sorry I can't be more helpful.<o:p></o:p></span></p>
<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"> politics is more difficult than physics.<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"> A. Einstein<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"> David Gene Morgan<br>
Electron Microscopy Center<br>
047D Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="http://iubemcenter.indiana.edu">http://iubemcenter.indiana.edu</a><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> 3dem <3dem-bounces@ncmir.ucsd.edu>
on behalf of Artemis Kosta <artemiskosta@gmail.com><br>
<b>Sent:</b> Monday, April 23, 2018 9:52 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Vitrobot humidifier problem</span><span style="font-family:"Calibri","sans-serif";color:#212121">
<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#212121">Dear colleagues,<br>
<br>
we are experiencing a problem getting the Vitrobot MarkIV humidifier to work.<br>
As soon as we try to increase the humidity in the chamber the system gives a message saying "empty" (in the humidity panel).<br>
<br>
We have tried emptying and refilling it again and removing any air bubble in the line but that has not fixed the problem.<br>
<br>
When we detach the cylinder from the Vitrobot, the water doesn't splash..<br>
Has anyone else had this problem and if so how did you solve it?<br>
<br>
Do you have a technical manual? We never managed to get one from FEI.<br>
<br>
Best regards<br>
Artemis Kosta</span><span style="font-family:"Calibri","sans-serif";color:#888888"><br>
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<span class="xgmail-hoenzb">-- </span></span><span class="xgmail-hoenzb"><span style="color:#888888"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#888888">Artemis Kosta<br>
Service de microscopie<br>
CNRS<br>
FR3479 Institut de Microbiologie de la Méditerranée<br>
31 chemin Joseph Aiguier<br>
13402 Marseille cedex 20<br>
France<br>
<a href="mailto:akosta@imm.cnrs.fr" target="_blank" id="LPlnk348058">akosta@imm.cnrs.fr</a><br>
0033 491 164055<br>
<a href="http://www.imm.cnrs.fr/microscopie/" target="_blank" id="LPlnk73044">http://www.imm.cnrs.fr/microscopie/</a></span><o:p></o:p></p>
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