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<div class="moz-forward-container">FIRST ANNOUNCEMENT:<br>
<p><span style="font-size:12.0pt;font-family:"Times New
Roman",serif"> --- THE EIGHTH BRAZIL SCHOOL FOR SINGLE
PARTICLE CRYO-EM ---</span><br>
</p>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif">To be held in Mariporã, SP Brazil </span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif"><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">27 Sept - 9 Oct 2018,</span> (<a
href="http://www.brazil-school.org" moz-do-not-send="true">www.brazil-school.org</a>)</span><br>
<br>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif">Our single-particle school is a unique and
intensive 12-day hands-on course/workshop, in which theory and
practice are tightly intertwined. <br>
The understanding of the principles of cryo-EM data processing
stands central in this event and a few cryo-EM pioneers will
explain the approaches to the participants to a level where they
can apply it themselves. <br>
The spectrum of topics covered suits attendees who are new to
the field as well as those for whom the technique is of growing
importance and who wish to acquire an in-depth understanding of
the fundamental principles.</span><br>
<br>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif">The final days of the meeting will be devoted
to advanced topics such as high-resolution refinements and the
processing of heterogeneous data sets ("4D cryo–EM”). Advanced
topics will include:</span><br>
<br>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif;mso-fareast-font-family: "Times New
Roman""><span style="mso-list:Ignore">1)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Optimizing data collection with direct
electrons detectors and exploiting movies. </span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">2)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Full data set automatic CTF correction.</span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">3)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Correction of anisotropic magnification.</span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">4)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Reaching atomic resolution without reference
bias: alignment-by-classification (ABC-4D) refinement
procedures.</span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">5)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Unbiased "Random Startup" of 3D and 4D
reconstructions.</span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">6)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">4D refinement procedures for high-resolution
results using IMAGIC and Frealign/cisTEM.</span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">7)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Fitting and refining atomic coordinates in
high-resolution cryo-EM maps (~4Å or better) using programs such
as REFMAC and Coot.</span><br>
<span style="font-size:12.0pt;font-family: "Times New
Roman",serif;mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">8)<span
style="font:7.0pt "Times New Roman""> </span></span></span><span
style="font-size:12.0pt;font-family:"Times New
Roman",serif">Validation of final results by reproducible
resolution criteria.</span><br>
<br>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif">Kind regards,</span><br>
<br>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif">Marin van Heel</span><br>
<br>
<span style="font-size:12.0pt;font-family:"Times New
Roman",serif">PS: We have experienced a very strong
interest in the 8th Brazil school even prior to this formal
announcement!<br>
The school is likely to be oversubscribed so don't delay your
registration.<span style="mso-spacerun:yes"> </span></span><br>
<pre class="moz-signature" cols="72">--
==============================================================
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
tel: +55-19-3518-2316
mobile +55-19-981809332
(041-19-981809332 TIM)
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
marin.vanheel(A_T)lnnano.cnpem.br
and: mvh.office(A_T)gmail.com
--------------------------------------------------
Emeritus Professor of Cryo-EM Data Processing
Leiden University
Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)
--------------------------------------------------
Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
email: m.vanheel(A_T)imperial.ac.uk
--------------------------------------------------
I receive many emails per day and, although I try,
there is no guarantee that I will actually read each incoming email. </pre>
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