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<p class="MsoNormal" style="background-color: white;">Dear colleagues,</p>
<p class="MsoNormal" style="background-color: white;">We are excited to announce the release of the latest version of our SPHIRE software suite (SPHIRE 1.0) (co-distributed with EMAN 2.21), which is now available from our website: <br class="">
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<a href="http://sphire.mpg.de/" target="_blank" class=""><span class="" style="color: blue;">http://sphire.mpg.de</span></a><br class="">
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Main new components and updates:<br class="">
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- SORT3D – A novel 3D sorting program based on novel concepts of reproducibility. We tested it on several high-resolution projects in our lab and were able to gain valuable and unparalleled insight into data heterogeneity. <br class="">
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- MERIDIEN – Rapid ML 3D refinement. We have introduced initialization of the program directly from 3D orientation parameters, as opposed to standard mode, which is initiated by an initial template structure. The new mode allows the user to rapidly perform
local refinements of data subsets obtained with SORT3D. The two programs, MERIDIEN and SORT3D, thus serve as a perfect tool for exploration of data set heterogeneity. <br class="">
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- We have streamlined the processing workflow and the GUI interface facilitating users’ interactions with the system. </p>
<p class="MsoNormal" style="background-color: white;">- The new release is accompanied by extensive documentation including tutorials explaining the underlying concepts.<o:p class=""></o:p></p>
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For any issues and inquiries, the SPHIRE team is accessible 24/7 <i class="">via</i> the mailing list. We sincerely hope that the updated SPHIRE 1.0 will meet your expectations and be a helpful advance in high-resolution cryo-EM.<br class="">
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Your SPHIRE development team<br class="">
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Toshio, Markus, Felipe, Thorsten, Christos, and Stefan – MPI Dortmund<br class="">
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Zhong and Pawel - UTH Texas<br class="">
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(Follow us on Twitter: <a href="https://twitter.com/SPHIRE_Package" target="_blank" class=""><span class="" style="color: blue;">https://twitter.com/SPHIRE_Package)</span></a><o:p class=""></o:p></p>
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Stefan Raunser, Ph.D.<br class="">
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Director, Department of Structural Biochemistry</div>
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<span class="" style="text-align: -webkit-auto;">Max Planck Institute of Molecular Physiology</span><br class="">
Otto-Hahn-Str. 11<br class="">
44227 Dortmund, Germany<br class="">
tel: +49-231-133-2300<br class="">
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and<br class="">
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Adjunct Professor, <span class="" style="text-align: -webkit-auto;">Faculty of Chemistry and </span><span class="" style="text-align: -webkit-auto;">Chemical Biology </span></div>
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Technical University Dortmund</div>
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Honorary Professor, Centre for Medical Biotechnology, Faculty of Biology</div>
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University Duisburg-Essen</div>
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