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<p>Hi Dr. Masich,</p>
<p>There are a few points to make here and two things that could
cause magnification problems in general.</p>
<p>Some points:<br>
</p>
<p>The magnification you read out in the user interface is the
magnification at the "flu screen." The magnification you read out
on the user interface when you lift the screen is the
magnification at the film plane. The actual magnification (or as
we commonly refer to it: pixel size) at the camera plane will
depend on the camera's position along the path of the beam. The
further along the beam path, the greater the magnification. <br>
</p>
<p>It is expected that you will have a higher mag at the camera
plane than what is read out on the user interface. From your
description however, it sounds like the mag that is read out on
the screen is fixed but the effective magnification at the camera
plane is changing (from 50kX to some other value). If this is
indeed the case, I would check on the following:<br>
</p>
<p>The magnification is determined by the excitation values of the
projection lenses. For any given magnification, each lens of the
projection system should have a specified current flowing through
it that would achieve that magnification at the given high tension
value. For CM systems, if you open up the service documentation
that is delivered with the system, a table of these currents is
available for reference. You can open up the "display currents"
page on the interface and compare them. For a fully functional
system the current values won't be exact, but very close. In my
experience, these values are trustworthy. In theory, it is also
possible to pull out the lens cable, put a current meter in
series, and measure the 'true current' in the event you need to
get into deeper troubleshooting.<br>
</p>
<p>If the currents are correct sometimes and not other times, then
you have a problem to troubleshoot. <br>
</p>
<p>The problem can be:</p>
<p>1) The HT circuit- there are several boards that govern the HT
and how it ties in to the lenses, any one of them could be
defective<br>
</p>
<p>2) The lens circuit- there are several boards that amplify the
signal and produce the current for each lens, any one of them
could be defective<br>
</p>
<p>3) Both circuits- this is less likely, but it could be more than
one board on each circuit causing this issue; if that were the
case I'd expect a more dramatic symptom<br>
</p>
<p>I hope this was helpful.</p>
<p>Best,</p>
Mark Ebrahim<br>
Subangstrom LLC<br>
<br>
<div class="moz-cite-prefix">On 2/20/2018 2:04 PM, Sergej Masich
wrote:<br>
</div>
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<![endif]--><!--StartFragment--><span class="" lang="EN-GB">Dear 3D-EM
community,</span>
<div class=""><br class="">
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<div class="">We have observed a strange phenomenon in our old FEI
CM200 microscope. For example, among many images acquired at the
set value of 38 kX, there were a few images having actual
magnification of 50 kX. The similar behaviour was observed for
other magnifications as well. Our very experienced engineer
could not explain the discrepancy. Can somebody advise what can
have caused the problem?</div>
<div class=""><br class="">
</div>
<div class="">Best regards,</div>
<div class=""><br class="">
</div>
<div class="">Sergej <br class="">
<div class="">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space;
-webkit-line-break: after-white-space;" class="">
<div style="color: rgb(0, 0, 0); font-family: Helvetica;
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text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;">
=============================<br class="">
Dr. Sergej Masich<br class="">
Dept. of Cell and Molecular Biology<br class="">
Karolinska Institutet<br class="">
171 77 Stockholm, Sweden<br class="">
tel: (+)46 - 8 - 524 873 61<br class="">
mobile: (+)46 - 736 - 833 693<br class="">
e-mail: <a href="mailto:Sergej.Masich@ki.se" class=""
moz-do-not-send="true">Sergej.Masich@ki.se</a></div>
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0px; widows: auto;"><br class="">
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</div>
<br class="">
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<br>
<pre wrap="">_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
</pre>
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