<div dir="ltr">Dear lists,<div><br></div><div>I am trying to continue a relion 3D auto-refine job but have the following errors:</div><div><br></div><div><p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">ERROR:</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">MlOptimiser::readStar: File --i cannot be read.</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">File: /programs/x86_64-linux/relion/2.1b1_cu9.0/src/ml_optimiser.cpp line: ERROR:</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">MlOptimiser::readStar: File --i cannot be read.</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">File: /programs/x86_64-linux/relion/2.1b1_cu9.0/src/ml_optimiser.cpp line: 676</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">ERROR:</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">MlOptimiser::readStar: File --i cannot be read.</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">File: /programs/x86_64-linux/relion/2.1b1_cu9.0/src/ml_optimiser.cpp line: 676</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">ERROR:</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">MlOptimiser::readStar: File --i cannot be read.676</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">ERROR:</span></p>
<p style="margin:0px;font-size:13px;line-height:normal;font-family:Menlo"><span style="font-variant-ligatures:no-common-ligatures">MlOptimiser::readStar: File --i cannot be read.</span></p></div><div><span style="font-variant-ligatures:no-common-ligatures"><br></span></div><div><span style="font-variant-ligatures:no-common-ligatures"><br></span></div><div>The relion version is 2.1b1_cu9.0. I used "--continue. --I Refine3D/job001/run_it017_optimiser.star ".</div><div><br></div><div>The --continue is not listed in the option list, not sure if it still valid.  </div><div><br></div><div>I also tried to continue the job through GUI but the input button for continue job is grayed out.</div><div><br></div><div>Any suggestions would be highly appreciated!</div><div><br></div><div>Best,</div><div><br></div><div>Zongli</div></div>