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<p class="MsoNormal"><span style="color:#1F497D">Dear David,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">I agree with Marin. It is a data synch error. This sometimes happens with our Gatan K2 camera and what we do is shut down the software and hardware and restart the hardware in order for it to resynch properly.
This would be what I would try first and likely what TVIPS would recommend. These systems are pushing lots of data through these channels and sometimes it gets messed up. Good luck.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Bob<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="color:windowtext">From:</span></b><span style="color:windowtext"> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Marin van Heel<br>
<b>Sent:</b> Thursday, October 26, 2017 7:40 AM<br>
<b>To:</b> David LIEBL <dliebl@imcb.a-star.edu.sg>; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] Camera CMOS malfunction<o:p></o:p></span></p>
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Dear David<br>
<br>
Apparently your F416 CMOS camera is subdivided into eight vertical stripes which are processed separately electronically. Stripe 5 and 6 (from 1-8) appear to have some electronic synchronization problem.
<br>
I would contact the manufacturer if I were you. (Tietz?)<br>
<br>
Cheers<br>
<br>
Marin<br>
<br>
==============================================<br>
<br>
On 26/10/2017 02:27, David LIEBL wrote:<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:#004080">Dear all, </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">Has anyone experienced similar CMOS malfunction/damage on TEM camera or could anyone tell what could have caused this kind of malfunction?</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">Attached is one illustrative image (flat field-corrected) plus dark frame image (uncorrected) for a reference. Both images were saved as 8-bit TIFFs (4K) and binned 4 (to reduce file size for email).
</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">Note the two vertical stripes - they look exactly like motion blur filter applied at zero angle with distance of 10 pixel (simulated in Photoshop).</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">Any suggestions?</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">Camera status description is also attached.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">Thank you for advice,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#004080">David</span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial",sans-serif"><a href="mailto:asyifah_rashid@bmsi.a-star.edu.sg"><b><span style="color:blue">David Liebl, PhD </span></b></a>::<b> IMB-IMCB Joint Electron Microscopy Suite Manager
</b>::<b> </b></span><b><span lang="EN-GB" style="font-size:9.0pt;font-family:"Arial",sans-serif">Institute of Molecular and Cell Biology (IMCB) and Institute of Medical Biology (IMB)
</span></b><span style="font-size:9.0pt;font-family:"Arial",sans-serif">::<b> Agency for Science, Technology and Research (A*STAR)
</b>:: 61 Biopolis Drive :: #B2 Proteos :: Singapore 138673 ::<b> </b>DID (65) 6407 0790 :: Fax (65) 6779 1117
</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial",sans-serif"><a href="http://www.a-star.edu.sg/imb/Tech-Platforms/IMB-IMCB-Joint-Electron-Microscopy-Suite.aspx"><span style="color:blue">www.a-star.edu.sg/imb/Tech-Platforms/IMB-IMCB-Joint-Electron-Microscopy-Suite.aspx</span></a></span><o:p></o:p></p>
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Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.<br>
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<br>
<br>
<o:p></o:p></span></p>
<pre>_______________________________________________<o:p></o:p></pre>
<pre>3dem mailing list<o:p></o:p></pre>
<pre><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><o:p></o:p></pre>
<pre><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><o:p></o:p></pre>
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<p><o:p> </o:p></p>
<pre>-- <o:p></o:p></pre>
<pre>==============================================================<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre> Prof Dr Ir Marin van Heel<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre> Laboratório Nacional de Nanotecnologia - LNNano<o:p></o:p></pre>
<pre> CNPEM/LNNano, Campinas, Brazil<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre> tel: +55-19-3518-2316<o:p></o:p></pre>
<pre> mobile +55-19-981809332 <o:p></o:p></pre>
<pre> (041-19-981809332 TIM)<o:p></o:p></pre>
<pre> Skype: Marin.van.Heel<o:p></o:p></pre>
<pre> email: marin.vanheel(A_T)gmail.com<o:p></o:p></pre>
<pre> marin.vanheel(A_T)lnnano.cnpem.br<o:p></o:p></pre>
<pre> and: mvh.office(A_T)gmail.com <o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>--------------------------------------------------<o:p></o:p></pre>
<pre> Emeritus Professor of Cryo-EM Data Processing<o:p></o:p></pre>
<pre> Leiden University<o:p></o:p></pre>
<pre> Mobile NL: +31(0)652736618<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre> Emeritus Professor of Structural Biology<o:p></o:p></pre>
<pre> Imperial College London<o:p></o:p></pre>
<pre> Faculty of Natural Sciences<o:p></o:p></pre>
<pre> email: m.vanheel(A_T)imperial.ac.uk<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>--------------------------------------------------<o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>I receive many emails per day and, although I try, <o:p></o:p></pre>
<pre>there is no guarantee that I will actually read each incoming email. <o:p></o:p></pre>
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