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<p><font size="+1" face="Calibri,sans-serif"><span style="font-size:
12pt; line-height: 115%;" lang="EN-US">Dear EM community,<br>
</span></font></p>
<p><font size="+1" face="Calibri,sans-serif"><span style="font-size:
12pt; line-height: 115%;" lang="EN-US">You may be aware, the
European Synchrotron Radiation Facility (ESRF) is currently
installing and commissioning a <font color="#cc0000"><b>Titan
Krios cryo electron microscope</b> </font>(cryo-EM)
equipped with a GATAN K2 detector. This new facility, <font
color="#cc0000"><b>ESRF ‘beamline’ CM0</b><b>1</b></font>,
will become operational in <font color="#990000"><font
color="#cc0000"><b>mid-November 2017</b></font>.</font>
With this is mind we would like to inform you here how to
apply for access to this new facility. </span><span
style="font-size: 12pt; line-height: 115%;" lang="EN-US"><br>
<br>
Applications for microscope access are to be made as Rolling
Access Proposals with the application system open from <b>28.09.2017</b>.
Initially, we will be accepting only projects that have been
previously evaluated using cryo-EM and for which suitable
proof of the sample quality is available. This proof has to be
included in the application for access and should consist of
cryoEM images taken on any other microscope showing that the
resolution obtained is sufficiently high to justify extending
this using the Titan Krios, coupled with 2D classification
and/or 3D reconstruction results. All proposals will be
evaluated by the ESRF MX Beam Time Allocation Panel (MX-BTAP)
which will allocate access based on the feasibility (see
above) and scientific merit.<br>
</span><span style="font-size: 12pt; line-height: 115%;"
lang="EN-US"><br>
Guidelines for writing a proposal can be found <a
href="http://www.esrf.fr/UsersAndScience/UserGuide/Applying/ProposalGuidelines/MXRollingCryoEM">HERE</a>.<font
color="#cc0000"> <b>Please make sure you use</b><b> </b><b>the
correct <u>"Experimental Method Form" template, which is
spec</u><u>ific for CryoEM</u><u> beamtime</u></b><u><b>
applications!</b></u></font></span><span
style="font-size: 12pt; line-height: 115%;" lang="EN-US"></span><span
style="font-size: 12pt; line-height: 115%;" lang="EN-US"><br>
<br>
The microscope (CM01) will be operated in a similar fashion to
the ESRF X-ray beam lines (i.e. 6 days per week during <a
href="http://www.esrf.fr/cms/live/live/en/sites/www/home/UsersAndScience/Accelerators/Operation/operationschedules.html">ESRF
operations periods</a></span><span style="font-size: 12pt;
line-height: 115%;" lang="EN-US">). An exception will be
during the planned ESRF-EBS shutdown when CM01 will continue
both to operate and to accept proposals. Initially, we will
schedule two experiments per week and we will reimburse a
maximum of two visitors per experiment.<br>
<br>
</span><span style="font-size: 12pt; line-height: 115%;"
lang="EN-US">Samples can be sent using CF100 dry-shippers as
for any other MX experiment, mentioning CM01 as the beam line
where the experiment will be carried out ( see <a
href="http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/Prepare_Your_Experiment">'How
to prepare your experiment</a>').</span><span
style="font-size: 12pt; line-height: 115%;" lang="EN-US"></span><span
style="font-size: 12pt; line-height: 115%;" lang="EN-US"><br>
<br>
Further information concerning the microscope and how to apply
for access can be found <a
href="http://www.esrf.fr/home/UsersAndScience/Experiments/MX/About_our_beamlines/CM01.html">HERE</a>
</span><a
href="http://www.esrf.fr/home/UsersAndScience/Experiments/MX/About_our_beamlines/CM01.html"><span
style="font-size: 12pt; line-height: 115%;" lang="EN-US"></span></a></font><span
style="font-size:12.0pt;line-height:115%;font-family:"Times
New Roman",serif; mso-ansi-language:EN-US" lang="EN-US"><font
face="Calibri,sans-serif">or by e-mail to <a
href="mailto:cryo-em@esrf.fr">cryo-em@esrf.fr</a>.<br>
<br>
Best regards,</font></span><br>
<span style="mso-ansi-language:EN-US" lang="EN-US"><br>
</span><span style="mso-ansi-language:EN-US" lang="EN-US"><font
size="-1" face="Calibri">Isai</font></span></p>
<span style="mso-ansi-language:EN-US" lang="EN-US"><font size="-1"
face="Calibri">On behalf of ESRF User Office.</font></span>
<pre class="moz-signature" cols="72">--
Eaazhisai KANDIAH
ESRF Cryo-EM beamline Scientist
Address: ESRF
71 Avenue des Martyrs, 38000 Grenoble
Telephone: +33 476 882691
Email: <a class="moz-txt-link-abbreviated" href="mailto:eaazhisai.kandiah@esrf.fr">eaazhisai.kandiah@esrf.fr</a>
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