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<p>Hi Garry,</p>
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when we used super resolution we use the same dose settings and frame times (8 e-/px/s, 30-40 frames) as we do using normal counting mode. This is done because the dose rate is measured per physical pixel. In super resolution you will measure (2 e-/superresolution
pixel/s, which corresponds to 8e-/physical pixel/s)<br>
<br>
Cheers<br>
Marcus<br>
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<div style="font-family:Tahoma; font-size:13px"><span class="x_signature">Marcus Fislage, PhD<br>
<br>
Howard Hughes Medical Institute (HHMI)<br>
Columbia University<br>
Department of Biochemistry and Biophysics<br>
Lab of Joachim Frank<br>
New York, NY<br>
</span><br>
<span class="x_signature"><font color="000000"><span class="x_signature">email address:
</span><span class="x_signature">mf2944@cumc.columbia.edu</span></font> <br>
Phone: 212.305.9524<br>
Fax: 212.305.9500<br>
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<div id="x_divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Garry P Morgan <Garry.Morgan@Colorado.EDU><br>
<b>Sent:</b> Wednesday, September 20, 2017 11:22:53 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] parameters for K2 super-resolution</font>
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<div class="PlainText">Hi,<br>
<br>
we’ve been collection SPA images with a K2 Summit on a Tecnai F30; so far we have only used counting mode at a nominal magnification of 31,000x. we’d like to try using super-resolution at a lower mag (i.e., nominal mag of 20,000x) to get a wider field of view
with a roughly equivalent pixel size. i’m wondering if anyone is doing this as a standard for their SPA data acquisition? and if so, how are you setting up your acquisition parameters? i.e., frame time and number of frames. because the dose is so much lower
at the lower mag, it takes a significantly longer total acquisition time (20-30 seconds) to achieve our desired sample dose. in order to keep the number of frames in the movie stack to ~40 (so that the movie stack file size isn’t too large), we would need
to increase the frame exposure time to between 0.5 and 1.0 sec. however, i’m worried this longer frame time would incorporate too much drift and essentially nullify the whole point of using the K2 and drift correction. i would appreciate any thoughts and advice
on this. <br>
<br>
one more question…Gatan recommends a dose rate no more than 10e-/ubpxel/sec going to the K2 while in counting mode. i believe we were told the dose rate should be 2.5e-/ubpixel/sec for super-resolution mode. can anyone tell me if this is true, or is the dose
rate of 10e-/ubpixel/sec in regular counting mode actually the same as 2.5e- dose rate in super-res mode because of the 0.5x binning? in other words, can i use the same beam intensity for for unbinned counting mode and super-res mode?<br>
<br>
thanks,<br>
Garry<br>
<br>
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