<html><head></head><body><div style="color:#000; background-color:#fff; font-family:Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:13px"><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span id="yui_3_16_0_ym19_1_1505449007313_10958">Just to Add to Masa's email-</span></div><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span><br></span></div><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span id="yui_3_16_0_ym19_1_1505449007313_11595">You may have to use a little bit more force to get a really thin ice on a single side blotting device.</span></div><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span><br></span></div><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span id="yui_3_16_0_ym19_1_1505449007313_11011">However, you can carry out "double blotting experiments" on a single grid i.e with one protein complex blotted softly leaving behind reasonable amount followed by addition the 2nd protein complex and then blot in the regular manner and plunge. This process can can be carried out with ease in a single side blotting device.</span></div><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span><br></span></div><div id="yui_3_16_0_ym19_1_1505449007313_10841"><span><br></span></div><div></div><div id="yui_3_16_0_ym19_1_1505449007313_10844"> </div><div class="signature" id="yui_3_16_0_ym19_1_1505449007313_10860"><div id="yui_3_16_0_ym19_1_1505449007313_10859"><font class="Apple-style-span" color="#0000FF" face="garamond, 'new york', times, serif" size="7" id="yui_3_16_0_ym19_1_1505449007313_10858"><span class="Apple-style-span" style="font-size: 28px; line-height: 19px;" id="yui_3_16_0_ym19_1_1505449007313_10857"><b id="yui_3_16_0_ym19_1_1505449007313_10856"><blockquote type="cite" style="color:rgb(0, 0, 0);font-family:Helvetica;font-weight:normal;line-height:normal;font-size:16px;" id="yui_3_16_0_ym19_1_1505449007313_10855"><div id="yui_3_16_0_ym19_1_1505449007313_10854"><div id="yui_3_16_0_ym19_1_1505449007313_10853"><div id="yui_3_16_0_ym19_1_1505449007313_10852"><span class="Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:Helvetica;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;font-size:16px;" id="yui_3_16_0_ym19_1_1505449007313_10851"><span class="Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:Helvetica;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;font-size:16px;" id="yui_3_16_0_ym19_1_1505449007313_10850"><div style="word-wrap:break-word;" id="yui_3_16_0_ym19_1_1505449007313_10849"><div id="yui_3_16_0_ym19_1_1505449007313_10848"><div id="yui_3_16_0_ym19_1_1505449007313_10847"><font class="Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4" id="yui_3_16_0_ym19_1_1505449007313_10846"><b id="yui_3_16_0_ym19_1_1505449007313_10845">Dr. Anindito Sen (Ph.D)</b></font></div><div id="yui_3_16_0_ym19_1_1505449007313_10863" dir="ltr"><br></div></div></div></span></span></div></div></div></blockquote><div id="yui_3_16_0_ym19_1_1505449007313_10898"><div id="yui_3_16_0_ym19_1_1505449007313_10897"><div id="yui_3_16_0_ym19_1_1505449007313_10896"><div id="yui_3_16_0_ym19_1_1505449007313_10895"><span class="Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:Helvetica;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;font-size:16px;" id="yui_3_16_0_ym19_1_1505449007313_10894"><span class="Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:Helvetica;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;font-size:16px;" id="yui_3_16_0_ym19_1_1505449007313_10893"><div style="word-wrap:break-word;" id="yui_3_16_0_ym19_1_1505449007313_10892"><div id="yui_3_16_0_ym19_1_1505449007313_10891"><div id="yui_3_16_0_ym19_1_1505449007313_10890"><font class="Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4"><b><br></b></font></div></div></div></span></span></div></div></div></div></b></span></font></div></div> <div class="qtdSeparateBR"><br><br></div><div class="yahoo_quoted" style="display: block;"> <div style="font-family: Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 13px;"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, Sans-Serif; font-size: 16px;"> <div dir="ltr"><font size="2" face="Arial"> On Friday, 15 September 2017, 9:05, Masahide Kikkawa <mkikkawa@gmail.com> wrote:<br></font></div> <br><br> <div class="y_msg_container"><div id="yiv4458218049"><div>One of the advantage of one-sided blotting is the “filtering” effect of the holey carbon membrane for filamentous/tubular samples. <div class="yiv4458218049">In this case, you can use lower concentrations of samples, because you can “concentrate” samples on the grid.<br clear="none" class="yiv4458218049"><div class="yiv4458218049">
<span class="yiv4458218049Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:'Hiragino Kaku Gothic Pro';font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;border-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:'Hiragino Kaku Gothic Pro';font-size:medium;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;border-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:'Hiragino Kaku Gothic Pro';font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;border-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:'Hiragino Kaku Gothic Pro';font-size:10px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;border-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:'Hiragino Kaku Gothic Pro';font-size:10px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;border-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;color:rgb(0, 0, 0);font-family:'Hiragino Kaku Gothic Pro';font-size:10px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;orphans:2;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;border-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><div class="yiv4458218049"><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"></span><div class="yiv4458218049" style="word-wrap:break-word;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"><span class="yiv4458218049Apple-style-span" style="border-collapse:separate;border-spacing:0px;color:rgb(0, 0, 0);font-family:Osaka;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-indent:0px;text-transform:none;orphans:2;white-space:normal;widows:2;word-spacing:0px;"></span></span></span></span></span></span><div class="yiv4458218049">---------------------------------------------------------------</div><div class="yiv4458218049">Masahide Kikkawa, M.D., Ph. D.</div><div class="yiv4458218049">Professor</div><div class="yiv4458218049">Department of Cell Biology & Anatomy</div><div class="yiv4458218049">Graduate School of Medicine</div><div class="yiv4458218049">The University of Tokyo</div><div class="yiv4458218049"><a rel="nofollow" shape="rect" class="yiv4458218049" target="_blank" href="http://structure.m.u-tokyo.ac.jp/">http://structure.m.u-tokyo.ac.jp/</a></div><div class="yiv4458218049">tel: +81-3-5841-3338</div><div class="yiv4458218049"><div class="yiv4458218049">fax: +81-3-5841-3339</div><div class="yiv4458218049">---------------------------------------------------------------</div></div><div class="yiv4458218049"><br clear="none" class="yiv4458218049"></div></div></div></div></div></div><br clear="none" class="yiv4458218049Apple-interchange-newline"></div><br clear="none" class="yiv4458218049Apple-interchange-newline"></div><br clear="none" class="yiv4458218049Apple-interchange-newline"></div><br clear="none" class="yiv4458218049Apple-interchange-newline"></div><br clear="none" class="yiv4458218049Apple-interchange-newline"><br clear="none" class="yiv4458218049Apple-interchange-newline">
</div>
<div><br clear="none" class="yiv4458218049"><blockquote class="yiv4458218049" type="cite"><div class="yiv4458218049yqt1165740783" id="yiv4458218049yqt62421"><div class="yiv4458218049">On Sep 15, 2017, at 12:31, Moni Banerjee <<a rel="nofollow" shape="rect" class="yiv4458218049" ymailto="mailto:banerjee.moni@gmail.com" target="_blank" href="mailto:banerjee.moni@gmail.com">banerjee.moni@gmail.com</a>> wrote:</div><br clear="none" class="yiv4458218049Apple-interchange-newline"><div class="yiv4458218049"><div class="yiv4458218049" dir="ltr"><div class="yiv4458218049MsoNormal"><span class="yiv4458218049" lang="EN-US">Dear all,<span class="yiv4458218049"></span></span></div><div class="yiv4458218049"><span class="yiv4458218049" lang="EN-US"> </span><br clear="none" class="yiv4458218049webkit-block-placeholder"></div><div class="yiv4458218049MsoNormal"><span class="yiv4458218049" lang="EN-US">I am wondering about the advantages (or
disadvantages) of one-sided vs two-sided blotting during sample freezing for
SPA. Do people have preferences for one or the other depending on the type of sample?
Does one-sided blotting lead to thicker ice in general? Any suggestion/comment
will be very welcome. <span class="yiv4458218049"></span></span></div><div class="yiv4458218049"><span class="yiv4458218049" lang="EN-US"> </span><br clear="none" class="yiv4458218049webkit-block-placeholder"></div><div class="yiv4458218049MsoNormal"><span class="yiv4458218049" lang="EN-US">Thanks,<span class="yiv4458218049"></span></span></div><div class="yiv4458218049"><span class="yiv4458218049" lang="EN-US"> </span><br clear="none" class="yiv4458218049webkit-block-placeholder"></div><div class="yiv4458218049MsoNormal"><span class="yiv4458218049" lang="EN-US">Manidipa<span class="yiv4458218049"></span></span></div>
<div class="yiv4458218049"><br clear="none" class="yiv4458218049"></div>-- <br clear="none" class="yiv4458218049"><div class="yiv4458218049gmail_signature"><div class="yiv4458218049" dir="ltr"><div class="yiv4458218049">------------------------<div class="yiv4458218049">Dr. Manidipa Banerjee, PhD</div><div class="yiv4458218049">Associate Professor</div><div class="yiv4458218049">School of Biological Sciences</div><div class="yiv4458218049">Indian Institute of Technology, Delhi</div><div class="yiv4458218049">New Delhi 110016</div><div class="yiv4458218049">India</div></div></div></div>
</div>
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