<div dir="ltr">Utrecht core lab provides these too:<div><br></div><div><a href="http://www.cellbiology-utrecht.nl/25-facilities/cell-microscopy-core.html">http://www.cellbiology-utrecht.nl/25-facilities/cell-microscopy-core.html</a><br></div><div><br></div><div>Cheers,</div><div>Sacha</div><div><br></div><div><br></div></div><div class="gmail_extra"><br><div class="gmail_quote">On Thu, Sep 14, 2017 at 2:35 PM, Wright, Elizabeth R. <span dir="ltr"><<a href="mailto:erwrigh@emory.edu" target="_blank">erwrigh@emory.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Hi Daniel,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">We use these…<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">BSA-treated tracers, EM grade (EMS, cat. no. 25486).
<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Good luck!<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Liz<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><u></u> <u></u></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Times New Roman",serif">----<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Elizabeth R. Wright, PhD<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Associate Professor<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Emory University<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><a href="tel:(404)%20727-4665" value="+14047274665" target="_blank">(404) 727-4665</a><u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><a href="mailto:erwrigh@emory.edu" target="_blank">erwrigh@emory.edu</a><u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><u></u> <u></u></span></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Levy Daniel <<a href="mailto:Daniel.Levy@curie.fr" target="_blank">Daniel.Levy@curie.fr</a>><br>
<b>Date: </b>Thursday, September 14, 2017 at 8:03 AM<br>
<b>To: </b>"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
<b>Subject: </b>[3dem] gold fiducials<u></u><u></u></span></p>
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<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal"><span lang="FR">Dear All,</span><u></u><u></u></p>
<p class="MsoNormal">The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?<u></u><u></u></p>
<p class="MsoNormal">Best<u></u><u></u></p>
<p class="MsoNormal">Daniel Lévy<u></u><u></u></p>
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