<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<br class="">
<div>
<blockquote type="cite" class="">
<div class="">On Aug 15, 2017, at 7:22 AM, Philip Koeck <<a href="mailto:Philip.Koeck@ki.se" class="">Philip.Koeck@ki.se</a>> wrote:</div>
<div class="">
<div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">I see many open questions:<br class="">
If it’s due to scattering outside the aperture, shouldn’t it depend strongly on the aperture size?<br class="">
</span></div>
</div>
</div>
</blockquote>
<div>The objective aperture basically positioned so it is in Fourier space (for eleastically scattered electrons). That is, the aperture is the equivalent of a low-pass filter at the resolution corresponding to its radius. The typical aperture is at high enough
resolution that this doesn't normally impinge on the resolutions studied in CryoEM. Physically it is removing some electrons which might otherwise contribute to background noise. </div>
<div><br class="">
</div>
<div>Once an electron has scattered inelastically (by more than a very small amount), it can no longer contribute coherently to the image, but its absence can (amplitude contrast and beam attenuation). Thus we'd like to get rid of it physically so it doesn't
contribute instead to background noise, hence we have objective apertures and energy filters.</div>
<br class="">
<blockquote type="cite" class="">
<div class="">
<div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">If it’s due to multiple scattering, it must depend strongly on specimen thickness.</span></div>
</div>
</div>
</blockquote>
<blockquote type="cite" class="">
<div class="">
<div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">If it’s due to inelastic scattering, why is it so weak (about 10%)? Inelastic scattering has a larger cross section than elastic, I believe.<o:p class=""></o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""></span></div>
</div>
</div>
</blockquote>
<div><br class="">
</div>
Clearly the attenuation of the beam is impacted strongly by specimen thickness. However, the effect you are referring to as amplitude contrast is due to the difference in scattering in one part of the specimen vs another. That is, uniform solvent added above
or below the feature producing contrast simply attenuates the beam uniformly, and doesn't produce "contrast".<br class="">
<br class="">
The way we handle it in the weak phase approximation, as a cos() term is an approximation, as you could argue that it should be treated as an exponential decay with thickness as it is in optics. However, since we're talking about contrast bewteen different
scatterers in the X-Y plane rather than total attenuation by the specimen, for single particle work, it's a good approximation. For tomography of thick specimens, however, we should probably be thinking about the issue a bit deeper.</div>
<div><br class="">
</div>
<div><br class="">
<blockquote type="cite" class="">
<div class="WordSection1" style="page: WordSection1;">
<div class="" style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;">
<span lang="EN-US" class="" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"><o:p class=""></o:p></span></div>
</div>
</blockquote>
<blockquote type="cite" class="">
<div class="">
<div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">All the best,<o:p class=""></o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Philip<o:p class=""></o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<b class=""><span style="font-size: 10pt; font-family: Tahoma, sans-serif;" class="">Från:</span></b><span style="font-size: 10pt; font-family: Tahoma, sans-serif;" class=""><span class="Apple-converted-space"> </span>Sacha De Carlo [<a href="mailto:sacha.decarlo@dectris.com" style="color: purple; text-decoration: underline;" class="">mailto:sacha.decarlo@dectris.com</a>]<span class="Apple-converted-space"> </span><br class="">
<b class="">Skickat:</b><span class="Apple-converted-space"> </span>den 15 augusti 2017 13:11<br class="">
<b class="">Till:</b><span class="Apple-converted-space"> </span>Philip Koeck<br class="">
<b class="">Kopia:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a><br class="">
<b class="">Ämne:</b><span class="Apple-converted-space"> </span>Re: [3dem] [TEM] amplitude contrast<o:p class=""></o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
A good reference which may provide you with an answer... I am sure many other people will provide more recent studies...<o:p class=""></o:p></div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
Enjoy !<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
Best,<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
Sacha<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
On Tue, Aug 15, 2017 at 12:55 PM, Philip Koeck <<a href="mailto:Philip.Koeck@ki.se" target="_blank" style="color: purple; text-decoration: underline;" class="">Philip.Koeck@ki.se</a>> wrote:<o:p class=""></o:p></div>
<div class="">
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Dear all.</span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Is there any agreement on the cause of amplitude contrast when imaging proteins in cryo-TEM?</span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Is it the second order term in the expansion of a weak phase object or is it due to inelastic scattering or the aperture or something else?</span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Al the best.</span><o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span><span style="color: rgb(136, 136, 136);" class=""><o:p class=""></o:p></span></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span lang="EN-US" style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Philip</span><span style="color: rgb(136, 136, 136);" class=""><o:p class=""></o:p></span></div>
</div>
</div>
<p class="MsoNormal" style="margin: 0cm 0cm 12pt; font-size: 12pt; font-family: 'Times New Roman', serif;">
<br class="">
_______________________________________________<br class="">
3dem mailing list<br class="">
<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a><br class="">
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.ncmir.ucsd.edu_mailman_listinfo_3dem&d=DwMGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=D5Ng_Q1_T1dxGU_ucMYh2ELS67C2X9-Nmht6hMpv54c&e=" target="_blank" style="color: purple; text-decoration: underline;" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><o:p class=""></o:p></p>
</div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<br class="">
<br clear="all" class="">
<o:p class=""></o:p></div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
--<span class="Apple-converted-space"> </span><o:p class=""></o:p></div>
<div class="">
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 18pt; font-family: Verdana, sans-serif;" class="">Sacha De Carlo, Ph.D.</span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class="">Business Development Manager EM</span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span class="Apple-converted-space"> </span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class="">Phone: </span><span style="font-size: 10pt; font-family: Verdana, sans-serif; color: gray;" class="">+41 56 500 21 90</span><span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class=""> </span><span style="font-size: 7.5pt; font-family: Arial, sans-serif; color: gray;" class="">|</span><span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class=""> Mobile: </span><span style="font-size: 10pt; font-family: Verdana, sans-serif; color: gray;" class="">+41
79 354 47 40</span><span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class=""> </span><span style="font-size: 7.5pt; font-family: Arial, sans-serif; color: gray;" class="">|</span><span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class=""> Email: <a href="mailto:sacha.decarlo@dectris.com" target="_blank" style="color: purple; text-decoration: underline;" class="">sacha.decarlo@dectris.com</a></span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 10pt; font-family: Verdana, sans-serif; color: gray;" class="">DECTRIS Ltd. | Taefernweg 1 | 5405 Baden-Daettwil | Switz</span><span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class="">erland | </span><span style="font-size: 7.5pt;" class=""><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.dectris.com&d=DwMGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=jp1D1kOx0e929S0Amj1bq_uNAow43N94Ukapqtgl4SE&e=" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="font-family: Verdana, sans-serif;" class="">www.dectris.com</span></a></span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.linkedin.com_company_5067919&d=DwMGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=0hpoaWi23s5qog4zlkwdfHGgWdTYk1uuvemLIiLmoyk&e=" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="text-decoration: none;" class=""><img border="0" id="_x0000_i1025" src="https://www.dectris.com/tl_files/root/signatur/linkein-ico1.png" alt="LinkedIn" class=""></span></a><o:p class=""></o:p></div>
</div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.facebook.com_pages_Dectris-2DLtd_623855944369304&d=DwMGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=DKYNEkGGPWIdJUt9K6bjY6BRWoGqWO1g1QqaJNFvSbs&e=" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="text-decoration: none;" class=""><img border="0" id="_x0000_i1026" src="https://www.dectris.com/tl_files/root/signatur/facebook-ico1.png" alt="facebook" class=""></span></a> <a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_DECTRIS-5FNews&d=DwMGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=uhL2MV0uSdqZSP0d1s5U8qKa55tzY2W8jtd3q87kCaY&e=" target="_blank" style="color: purple; text-decoration: underline;" class=""><span style="text-decoration: none;" class=""><img border="0" id="_x0000_i1027" src="https://www.dectris.com/tl_files/root/signatur/twitter-ico1.png" class=""></span></a><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<img border="0" id="_x0000_i1028" src="https://www.dectris.com/tl_files/root/signatur/dectris_plus%20slogan.png" class=""><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class="">Confidentiality Note: This message is intended only for the use of the named </span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 7.5pt; font-family: Verdana, sans-serif; color: gray;" class="">recipient(s) </span><span style="font-size: 10pt; font-family: Verdana, sans-serif; color: gray;" class="">and may contain confidential and/or privileged information. If
you </span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 10pt; font-family: Verdana, sans-serif; color: gray;" class="">are not the intended recipient, please contact the sender and delete the message.</span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<span style="font-size: 10pt; font-family: Verdana, sans-serif; color: gray;" class="">Any unauthorized use of the information contained in this message is prohibited.</span><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">
<o:p class=""> </o:p></div>
</div>
</div>
</div>
</div>
<span style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<span style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">3dem
mailing list</span><br style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.ncmir.ucsd.edu_mailman_listinfo_3dem&d=DwICAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=D5Ng_Q1_T1dxGU_ucMYh2ELS67C2X9-Nmht6hMpv54c&e=" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.ncmir.ucsd.edu_mailman_listinfo_3dem&d=DwICAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=SItasz8QqEHCjBlzrGdn0Yuj4QjEyPcs73x7tNF_d6Y&s=D5Ng_Q1_T1dxGU_ucMYh2ELS67C2X9-Nmht6hMpv54c&e=</a><span style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class=""></span></div>
</blockquote>
</div>
<br class="">
<div class="">
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-east-asian: normal; font-variant-position: normal; line-height: normal; border-spacing: 0px;">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<div class="">
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
----------------------------------------------------------------------------</div>
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
Steven Ludtke, Ph.D.</div>
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
Professor, Dept of Biochemistry and Mol. Biol. (<a href="http://www.bcm.edu/biochem" class="">www.bcm.edu/biochem</a>)</div>
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
Co-Director National Center For Macromolecular Imaging <span class="Apple-converted-space"> </span>(<a href="http://ncmi.bcm.edu" class="">ncmi.bcm.edu</a>)</div>
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
Co-Director CIBR Center <span class="Apple-converted-space"> </span>(<a href="http://www.bcm.edu/research/cibr" class="">www.bcm.edu/research/cibr</a>)</div>
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
Baylor College of Medicine<span class="Apple-converted-space"> </span> <span class="Apple-converted-space"> </span></div>
<div style="margin: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; " class="">
<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a></div>
</div>
<div class=""><br class="">
</div>
</div>
</span></div>
</span></div>
</span></div>
</span><br class="Apple-interchange-newline">
</div>
<br class="Apple-interchange-newline">
<br class="Apple-interchange-newline">
</div>
<br class="">
</body>
</html>