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Dear all,<br>
<br>
Please find bellow the description of a joint PhD position in CryoEM
between ESRF and SANOFI<br>
<br>
Best regards<br>
<br>
Guy Schoehn<br>
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<p style="text-align:justify"><span style="font-size:11.0pt;
font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB">PhD thesis title: High resolution Cryo- Electron
Microscopy of Antibody / Antigen complexes<o:p></o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0mm;margin-bottom:.0001pt;text-align:
justify;mso-layout-grid-align:none;text-autospace:none"><span
style="font-family:"Arial",sans-serif;color:black;mso-ansi-language:EN-GB;mso-fareast-language:JA"
lang="EN-GB">More and more research focus is being put on
biologics and their development as drugs. These molecules are
usually large and flexible and they can interact with various
targets located on the surface of cells; Bi-specific
antibodies are an example that can potentially bind to two
different antigens and which have gained attention due to
their potential application in cancer immunotherapy and drug
delivery. To fully understand their mechanism, structural
knowledge at an atomic level is needed.<o:p></o:p></span></p>
<pre><span style="font-size:11.0pt;font-family:"Arial",sans-serif;
mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;color:black;
mso-ansi-language:EN-GB;mso-fareast-language:JA" lang="EN-GB">While low resolution studies can be obtained using traditional microscopy, high resolution studies of these interactions are usually performed by protein crystallography (or by Hydrogen Deuterium Exchange studies) using fragments of both antibody and antigen. The ESRF is in progress of installing a Krios microscope which, coupled with a direct electron detector and a Volta phase plate will enable us to determine the full structure of the complexes.<o:p></o:p></span></pre>
<p class="MsoNormal" style="text-align:justify"><span
style="font-family:"Arial",sans-serif;color:black;mso-ansi-language:EN-GB;mso-fareast-language:JA"
lang="EN-GB">The successful candidate</span><span
style="font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB"> will characterise Ag/Biologics complexes from
Sanofi, using state-of-the-art microscopes at the cryo-EM
platform of the Partnership for Structural Biology (PSB) and
will determine the high-resolution structure of one or more of
these complexes. <o:p></o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0mm;margin-bottom:.0001pt;text-align:
justify;line-height:normal"><span
style="font-family:"Arial",sans-serif;
mso-fareast-font-family:"Times New
Roman";mso-ansi-language:EN-GB;mso-fareast-language: FR"
lang="EN-GB">The PhD thesis will last 3 years starting in late
autumn 2017, in a collaboration between Sanofi (Vitry sur
Seine, France) and the ESRF (Grenoble, France) under the
co-supervision of Dr. Magali Mathieu (Sanofi) and Dr. Gordon
Leonard (ESRF). This thesis will be CIFRE (Conventions
Industrielles de Formation par la REcherche) and the student
will be a Sanofi employee (CDD) for the whole duration of the
thesis. Most of his/her work will be performed at the ESRF.<o:p></o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0mm;margin-bottom:.0001pt;text-align:
justify;line-height:normal"><span
style="font-family:"Arial",sans-serif;
mso-fareast-font-family:"Times New
Roman";mso-ansi-language:EN-GB;mso-fareast-language: FR"
lang="EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0mm;margin-bottom:.0001pt;text-align:
justify;line-height:normal"><span
style="font-family:"Arial",sans-serif;
mso-fareast-font-family:"Times New
Roman";mso-ansi-language:EN-GB;mso-fareast-language: FR"
lang="EN-GB">The applicant must have a Master Degree (Master2
or MSc) in integrated structural biology or biophysics, some
knowledge in cryo-electron microscopy and in molecular and
cell biology. He/she should be a team player and have good
communication skills.<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span
style="font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB">CV and accompanying letter should be sent to </span><a
moz-do-not-send="true" href="mailto:magali.mathieu@sanofi.com"><span
style="font-family:
"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB">magali.mathieu@sanofi.com</span></a><span
style="font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB"> AND </span><a moz-do-not-send="true"
href="mailto:christoph.mueller_dieckmann@esrf.fr"><span
style="font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB">christoph.mueller_dieckmann@esrf.fr</span></a><span
style="font-family:"Arial",sans-serif;mso-ansi-language:EN-GB"
lang="EN-GB"> before July 7, 2017.<o:p></o:p></span></p>
<p><br>
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