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Dear List,
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<div class="">I have a position open in my Center at Washington University School of Medicine for a Cryo-EM Specialist. Details are below. Interested parties please apply at
<a href="http://jobs.wustl.edu" class="">jobs.wustl.edu</a> and search for job ID 36721.</div>
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<div class="">Thanks,</div>
<div class="">James</div>
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<div class=""><b class="">James A.J. Fitzpatrick, Ph.D.</b><br class="">
Scientific Director, Washington University Center for Cellular Imaging<br class="">
Associate Professor, Departments of Neuroscience, Cell Biology & Physiology and Biomedical Engineering<br class="">
Washington University in St. Louis<br class="">
tel: (314) 747-0838<br class="">
email: <a href="mailto:fitzp@wustl.edu" class="">fitzp@wustl.edu</a><br class="">
web: <a href="http://wucci.wustl.edu" class="">http://wucci.wustl.edu</a></div>
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<b class="">POSITION TITLE: </b>Cryo-Electron Microscopy Research Specialist<img alt="" apple-inline="yes" id="D8D66B9F-7EC4-4D68-98E5-6CB2743E6087" src="cid:C8D743A5-068A-4340-8247-A8F989C89056@wucon.wustl.edu" class=""> (Job ID: 36721)<br class="">
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<b class="">POSITION SUMMARY: </b>The Cryo-Electron Microscopy Specialist will support the operation of its new Titan Krios G3 Cryo-EM equipped with a Direct Electron Detector for single particle and tomography studies. This position will work with other EM
staff under the supervision of the Center Director in operation of the electron microscopy component of the Center for Cellular Imaging (<a href="http://wucci.wustl.edu/" class="">http://wucci.wustl.edu/</a>). The Cryo-EM Specialist will also have the opportunity
to work on correlative microscopy technology development projects as a part of the Centers technology development efforts.<br class="">
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<b class="">PRIMARY DUTIES AND RESPONSIBILITIES:</b><br class="">
<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>1. User Training - train users in sample preparation and imaging techniques for single-particle cryo-EM.<br class="">
<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Introduce and orient new users on the safe operation of the Titan Krios cryo-EM platform.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Provide assistance and troubleshooting on cryo-EM experiments.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Provide advanced level instruction on an individual level with undergraduate students, graduate students, post-docs, and collaborators on the appropriate methods to vitrify samples
(both for single particle and tomography)<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Develop reference tools for lab staff including handouts.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>2. Cryo-EM Service - Prepare samples for single particle cryo-EM and collect and reconstruct images for University researchers to help to prepare data for publication and presentations.<br class="">
<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Prepare biological samples for cryo-EM (purified proteins and cells) using plunge freezing techniques.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Data collection and evaluation of data for in-house and external users carrying out structural biology-focused projects.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Evaluate quality of data obtained by users.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Interface and collaborate with other EM staff in the Center to facilitate preliminary screening of samples via negative staining techniques.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Basic 3D image processing of single particle and/or tomography datasets.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Interface with High Performance Computing team, who will assist with computational needs for optimal data storage and data processing.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Implement data collection strategies that will benefit the burgeoning cryo-EM user community at Washington University.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Prepare data for publication; grant applications, posters and other presentations using digital facilities.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Prepare publication quality micrographs or images/movies for presentations<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Assist in preparation of manuscripts, grants, progress reports and annual protocols. <br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Maintain lab notebook, including experimental design, time, methods, materials and results.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>3. Cryo-EM Equipment Maintenance - maintenance of the cryo-EM and sample preparation equipment.<br class="">
<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Perform general maintenance of the Titan Krios cryo-EM microscope.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Liaise with manufacturer (FEI/Thermo Fishr) with regard to instrument preventative maintenance.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Maintain ancillary, cryo-EM sample preparation equipment such as Vitrobot, glow discharger etc.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Load samples and fine-tune microscope prior to single particle or tomography data collection runs.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Maintain records of equipment usage and report maintenance issues of equipment.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Ensure assigned areas, and various pieces of equipment are clean and prepared.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Use scheduling software to prepare monthly invoices for facility user recharges and track of equipment usage to prepare monthly status reports for Director.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Ensure Center is in compliance with EH&S protocols and procedures.<br class="">
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<b class="">EDUCATION:</b> Master’s degree in a related field with 7 years of experience in a laboratory setting or equivalent combination of relevant education and work experience equaling at least 10 years.<br class="">
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<b class="">PREFERRED QUALIFICATIONS:</b><br class="">
<img alt="" apple-inline="yes" id="75B497E6-2560-455C-8334-9E0F13CB6132" src="cid:C8D743A5-068A-4340-8247-A8F989C89056@wucon.wustl.edu" class=""><br class="">
<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- At least 2-5 years of cryo-EM experience, with expertise in sample freezing, sample handling, microscope use, and data evaluation and analysis.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Firm understanding of the principles of Transmission Electron Microscopy (TEM)<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Demonstrated skill and experience in the high-resolution operation of cryo electron microscopes<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- General understanding of single-particle cryo-EM workflow, experience in cryo-electron tomography, Cs correction and Phase contrast systems would be added benefits.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Theoretical and practical knowledge of biology or related scientific field and of tissue structure on an anatomical, cellular, and molecular level.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Ability to contribute to experimental design and assist with the analysis and interpretation of results.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Proven organizational and time management skills to successfully set priorities, meet established deadlines and recognize new problems with constantly changing priorities and frequent
interruptions.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Ability to diplomatically and professionally interact with all levels of University faculty and staff and external contacts.<br class="">
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<div class=""><span class="Apple-tab-span" style="white-space: pre;"></span>- Proven ability to perform minor maintenance and alignment of electron microscopes.<br class="">
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<img alt="" apple-inline="yes" id="616F3A0D-C38F-4F54-B8D4-61E8614E741D" src="cid:C8D743A5-068A-4340-8247-A8F989C89056@wucon.wustl.edu" class=""><br class="">
<b class="">SALARY:</b> The hiring range for this position is commensurate with experience.<br class="">
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<b class="">BENEFITS:</b> This position is eligible for full-time benefits. Please visit our website at http://hr.wustl.edu to view a summary of benefits.</div>
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