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<p><font face="Calibri">Hi!</font></p>
<p><font face="Calibri"> Settling into two orientations should take
time, perhaps there is a way to shorten the time the sample
spends on the grid before being frozen. <br>
</font></p>
<p><font face="Calibri">What is the driving force for the
orientation preference? Support film properties, draining of the
liquid film - perhaps playing with the type of support or
blotting manually might help?</font><br>
</p>
Best,<br>
<br>
Ilya.<br>
<div class="moz-cite-prefix">On 17-May-17 14:46, Yang Li wrote:<br>
</div>
<blockquote
cite="mid:CA+UrRchYpprX7E1xVDSGKfvMdMZ2TbXs-YLw8G6nx3Qgt=whxA@mail.gmail.com"
type="cite">
<div dir="ltr">Dear colleagues,
<div><br>
</div>
<div>We have a protein sample that suffers from severe
orientation preference, that most of the particles cluster
into two distinct orientations. This way we have to collect
large amounts of data in order to obtain enough effective
particles, which hiders us from reaching high resolution. We
have tried to make thicker ice or adding tiny amount of
detergent such as Tween20, but not working very well so far. I
wonder if there are any tricks we can try to overcome this
orientation preference issue? Thank you in advance for
suggestions!</div>
<div><br>
</div>
<div>Best,</div>
<div>Yang </div>
</div>
<br>
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