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<p>Dear Rado,</p>
<p><br>
</p>
<p>To firmly answer your question, I tried 5 accessible ones of all
6 VPPs at three different positions (#3, 30, 60). The behavior are
the same, with a variable degree. In deed, there was more than a
month delay from VPP delivery to installation due to engineer
schedule. However, it was sealed in the container. Thanks for the
input.</p>
<p><br>
</p>
<p>Best Regards,</p>
<p>Jian<br>
</p>
<br>
<div class="moz-cite-prefix">On 01/27/2017 01:29 AM, Danev, Radostin
wrote:<br>
</div>
<blockquote
cite="mid:F2D11EA155FD0643B7E705B99EC78E3B250CF082@MSX-MBS2.w2k.biochem.mpg.de"
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<div style="direction: ltr;font-family: Tahoma;color:
#000000;font-size: 10pt;">Hi Jian,<br>
<br>
If the CTF rings are moving outwards (make sure you are
underfocus!) that means positive charging. The reason is most
probably contamination on the phase plate film. If the heater is
working properly (voltage ~ 4.0V, current ~ 25 mA) then the
contamination is pre-existing, deposited during manufacturing,
transport or handling of the phase plate. Are all VPP slots
showing the same behavior?<br>
<br>
Cheers,<br>
<br>
Rado<br>
<br>
<div style="font-family: Times New Roman; color: #000000;
font-size: 16px">
<hr tabindex="-1">
<div id="divRpF484241" style="direction: ltr;"><font
face="Tahoma" color="#000000" size="2"><b>From:</b> 3dem
[<a class="moz-txt-link-abbreviated" href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>] on behalf of Sacha De Carlo
[<a class="moz-txt-link-abbreviated" href="mailto:sacha.decarlo@dectris.com">sacha.decarlo@dectris.com</a>]<br>
<b>Sent:</b> Thursday, January 26, 2017 8:32 AM<br>
<b>To:</b> jamon<br>
<b>Cc:</b> <a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<b>Subject:</b> Re: [3dem] FEI-VPP phase change to
opposite direction<br>
</font><br>
</div>
<div>
<div dir="ltr">Could it be that the holder, with the new
VPP, is no longer sitting in the back focal plane, where
it should be?
<div>In any case, these are things to check with you FEI
service engineer.</div>
<div><br>
</div>
<div>All the best,</div>
<div>Sacha</div>
<div><br>
</div>
<div><br>
</div>
</div>
<div class="gmail_extra"><br>
<div class="gmail_quote">On Thu, Jan 26, 2017 at 6:11 AM,
jamon <span dir="ltr"><<a moz-do-not-send="true"
href="mailto:jamonshi@gmail.com" target="_blank">jamonshi@gmail.com</a>></span>
wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0
.8ex; border-left:1px #ccc solid; padding-left:1ex">
Dear Lister,<br>
<br>
We just changed our FEI Volta Phase Plate on Krios.
But after activation, the phase changed to opposite
direction, the FFT rings moves outward instead of
inwards as it should be. The gold particles all looks
all white as if in overfocus. I had never seen such
behavior with previous two VPPs we had. Did anybody
have similar experience? Thanks in advance.<span
class="HOEnZb"><font color="#888888"><br>
<br>
-- <br>
Best Regards,<br>
Jian SHI<br>
Manager, Cryo Electron Microscopy Facility<br>
Center for BioImaging Science<br>
National University of Singapore<br>
<br>
<br>
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</div>
<br>
<br clear="all">
<div><br>
</div>
-- <br>
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