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    <p>Dear Rado,</p>
    <p><br>
    </p>
    <p>To firmly answer your question, I tried 5 accessible ones of all
      6 VPPs at three different positions (#3, 30, 60). The behavior are
      the same, with a variable degree. In deed, there was more than a
      month delay from VPP delivery to installation due to engineer
      schedule. However, it was sealed in the container. Thanks for the
      input.</p>
    <p><br>
    </p>
    <p>Best Regards,</p>
    <p>Jian<br>
    </p>
    <br>
    <div class="moz-cite-prefix">On 01/27/2017 01:29 AM, Danev, Radostin
      wrote:<br>
    </div>
    <blockquote
cite="mid:F2D11EA155FD0643B7E705B99EC78E3B250CF082@MSX-MBS2.w2k.biochem.mpg.de"
      type="cite">
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      <div style="direction: ltr;font-family: Tahoma;color:
        #000000;font-size: 10pt;">Hi Jian,<br>
        <br>
        If the CTF rings are moving outwards (make sure you are
        underfocus!) that means positive charging. The reason is most
        probably contamination on the phase plate film. If the heater is
        working properly (voltage ~ 4.0V, current ~ 25 mA) then the
        contamination is pre-existing, deposited during manufacturing,
        transport or handling of the phase plate. Are all VPP slots
        showing the same behavior?<br>
        <br>
        Cheers,<br>
        <br>
        Rado<br>
        <br>
        <div style="font-family: Times New Roman; color: #000000;
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          <div id="divRpF484241" style="direction: ltr;"><font
              face="Tahoma" color="#000000" size="2"><b>From:</b> 3dem
              [<a class="moz-txt-link-abbreviated" href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>] on behalf of Sacha De Carlo
              [<a class="moz-txt-link-abbreviated" href="mailto:sacha.decarlo@dectris.com">sacha.decarlo@dectris.com</a>]<br>
              <b>Sent:</b> Thursday, January 26, 2017 8:32 AM<br>
              <b>To:</b> jamon<br>
              <b>Cc:</b> <a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
              <b>Subject:</b> Re: [3dem] FEI-VPP phase change to
              opposite direction<br>
            </font><br>
          </div>
          <div>
            <div dir="ltr">Could it be that the holder, with the new
              VPP, is no longer sitting in the back focal plane, where
              it should be?
              <div>In any case, these are things to check with you FEI
                service engineer.</div>
              <div><br>
              </div>
              <div>All the best,</div>
              <div>Sacha</div>
              <div><br>
              </div>
              <div><br>
              </div>
            </div>
            <div class="gmail_extra"><br>
              <div class="gmail_quote">On Thu, Jan 26, 2017 at 6:11 AM,
                jamon <span dir="ltr"><<a moz-do-not-send="true"
                    href="mailto:jamonshi@gmail.com" target="_blank">jamonshi@gmail.com</a>></span>
                wrote:<br>
                <blockquote class="gmail_quote" style="margin:0 0 0
                  .8ex; border-left:1px #ccc solid; padding-left:1ex">
                  Dear Lister,<br>
                  <br>
                  We just changed our FEI Volta Phase Plate on Krios.
                  But after activation, the phase changed to opposite
                  direction, the FFT rings moves outward instead of
                  inwards as it should be. The gold particles all looks
                  all white as if in overfocus. I had never seen such
                  behavior with previous two VPPs we had. Did anybody
                  have similar experience? Thanks in advance.<span
                    class="HOEnZb"><font color="#888888"><br>
                      <br>
                      -- <br>
                      Best Regards,<br>
                      Jian SHI<br>
                      Manager, Cryo Electron Microscopy Facility<br>
                      Center for BioImaging Science<br>
                      National University of Singapore<br>
                      <br>
                      <br>
                      ______________________________<wbr>_________________<br>
                      3dem mailing list<br>
                      <a moz-do-not-send="true"
                        href="mailto:3dem@ncmir.ucsd.edu"
                        target="_blank">3dem@ncmir.ucsd.edu</a><br>
                      <a moz-do-not-send="true"
                        href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem"
                        rel="noreferrer" target="_blank">https://mail.ncmir.ucsd.edu/ma<wbr>ilman/listinfo/3dem</a><br>
                    </font></span></blockquote>
              </div>
              <br>
              <br clear="all">
              <div><br>
              </div>
              -- <br>
              <div class="gmail_signature">
                <div><span style="font-family:Verdana; font-size:large">Sacha
                    De Carlo, Ph.D.</span></div>
                <div><font face="Verdana" size="1"><span
                      style="color:rgb(128,128,128)">Business
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