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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi Jian,<br>
<br>
If the CTF rings are moving outwards (make sure you are underfocus!) that means positive charging. The reason is most probably contamination on the phase plate film. If the heater is working properly (voltage ~ 4.0V, current ~ 25 mA) then the contamination
is pre-existing, deposited during manufacturing, transport or handling of the phase plate. Are all VPP slots showing the same behavior?<br>
<br>
Cheers,<br>
<br>
Rado<br>
<br>
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<div id="divRpF484241" style="direction: ltr;"><font size="2" color="#000000" face="Tahoma"><b>From:</b> 3dem [3dem-bounces@ncmir.ucsd.edu] on behalf of Sacha De Carlo [sacha.decarlo@dectris.com]<br>
<b>Sent:</b> Thursday, January 26, 2017 8:32 AM<br>
<b>To:</b> jamon<br>
<b>Cc:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] FEI-VPP phase change to opposite direction<br>
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<div dir="ltr">Could it be that the holder, with the new VPP, is no longer sitting in the back focal plane, where it should be?
<div>In any case, these are things to check with you FEI service engineer.</div>
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<div>All the best,</div>
<div>Sacha</div>
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<div class="gmail_extra"><br>
<div class="gmail_quote">On Thu, Jan 26, 2017 at 6:11 AM, jamon <span dir="ltr"><<a href="mailto:jamonshi@gmail.com" target="_blank">jamonshi@gmail.com</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex; border-left:1px #ccc solid; padding-left:1ex">
Dear Lister,<br>
<br>
We just changed our FEI Volta Phase Plate on Krios. But after activation, the phase changed to opposite direction, the FFT rings moves outward instead of inwards as it should be. The gold particles all looks all white as if in overfocus. I had never seen such
behavior with previous two VPPs we had. Did anybody have similar experience? Thanks in advance.<span class="HOEnZb"><font color="#888888"><br>
<br>
-- <br>
Best Regards,<br>
Jian SHI<br>
Manager, Cryo Electron Microscopy Facility<br>
Center for BioImaging Science<br>
National University of Singapore<br>
<br>
<br>
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