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<p class="MsoNormal"><span lang="EN-GB">Dear List, dear </span>Benoît,</p>
<p class="MsoNormal">I have some different experience that can be wrong though. A colleague and a good friend of mine from Albuquerque successfully used his plasma cleaner to glow-discharge carbon films. I decided to copy his settings in our Lab and ordered
the same model. However, that model could not be sold to European customers due to some regulations. I purchased more expensive model from the same supplier. It did not work! As the result of “glow discharge”, I got empty grids, no traces of carbon. To make
a long story short, we installed a needle valve and a gauge to control the vacuum degree. We got hydrophilic carbon at poorer vacuum and no carbon at better vacuum. Since then, I used the plasma cleaner for many years to prepare hydrophilic carbon surface.</p>
<p class="MsoNormal">In my opinion, this also makes sense. The ions in plasma have longer free path at higher vacuum. As the result, they have higher energy that can result in more efficient removal of the “dirt” from the surface.</p>
<p class="MsoNormal">I never read any confirmation of my “theory” that can be completely wrong but I hope it will contribute to the discussion.<o:p></o:p></p>
<p class="MsoNormal"><o:p>Sincerely,</o:p></p>
<div>Sergej Masich</div>
<p class="MsoNormal">=============================<br>
Dr. Sergej Masich<br>
Dept. of Cell and Molecular Biology<br>
Karolinska Institutet<br>
Box 285<br>
171 77 Stockholm, Sweden<br>
tel: (+)46 - 8 - 524 873 61<br>
mobile: (+)46 - 736 - 833 693<br>
e-mail: <a href="mailto:Sergej.Masich@ki.se">Sergej.Masich@ki.se</a><br>
</p>
</div>
<br>
<div>
<div>On 20 Sep 2016, at 18:33, <<a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a>> <<a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a>> wrote:</div>
<br class="Apple-interchange-newline">
<blockquote type="cite">
<div style="font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
Hi David,<br>
<br>
This could be right. However I doubt it. Within 20 to 30 minutes we can completely disintegrate the carbon of quantifoil grids with the glow discharge machine set so that it glows strongly without sparkling.<br>
<br>
Cheers<br>
Ben<br>
<br>
________________________________________<br>
Von: Morgan, David Gene [<a href="mailto:dagmorga@indiana.edu">dagmorga@indiana.edu</a>]<br>
Gesendet: Dienstag, 20. September 2016 18:12<br>
An: Zuber, Benoît (ANA);<span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
Betreff: Re: glow discharge vs plasma cleaning<br>
<br>
Ben,<br>
<br>
<br>
Maybe someone else on the list can shed some light on this, but I don't know whether the strength of the plasma created in an EM "glow discharge device" is comparable to that of what is called a "plasma cleaner." I suspect not, but could be wrong.<br>
<br>
<br>
That said, over zealous use of a plasma cleaner can remove _all_ the carbon from an EM grid (I have done this with a lacy carbon grid), so having too much power can be a bad thing...<br>
<br>
<br>
--<br>
David Gene Morgan<br>
Electron Microscopy Center<br>
047D Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="http://iubemcenter.indiana.edu">http://iubemcenter.indiana.edu</a><br>
________________________________<br>
From: <a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a> <<a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a>><br>
Sent: Tuesday, September 20, 2016 12:06 PM<br>
To: Morgan, David Gene; <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
Subject: Re: glow discharge vs plasma cleaning<br>
<br>
Hi David and Luiza,<br>
<br>
Many thanks for the explanations. OK this « makes sense ». But it is a shame that people use two different words for the same thing (good job from the marketing team though ! ). It would be much better to use only one term and then give specific gaz conditions.
So many people are convinced that glow discharge and plasma cleaning are two different things and that you need two different machines.<br>
We have a so-called glow discharge machine from defunct Balzers. It still functions perfectly well, it looks like what people describe as a glow discharge machine : a glass cylinder with two metal plates below and on top, and it has an needle-valve inlet. We
typically let the inlet in contact with the air and let a tiny bit of air flow in to get a constant pressure in the chamber and thereby have a reproducible procedure. We can quite precisely control the pressure that we want inside. However if we want to use
whatever gas mixture instead of good old nitrogen and oxygen, we can just buy and connect the relevant gas bottle(s). No need to purchase a new machine.<br>
If I listened to what so many people told me, I would have long bought another machine, which would probably have been a waste of money and lab space. I think people should be more aware of this before spending their (tax payer ?) money.<br>
<br>
Cheers<br>
Benoît<br>
<br>
<br>
De : "Morgan, David Gene" <<a href="mailto:dagmorga@indiana.edu">dagmorga@indiana.edu</a>><br>
Date : mardi, 20 septembre 2016 17:51<br>
À : Benoit Zuber <<a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a>><br>
Objet : Re: glow discharge vs plasma cleaning<br>
<br>
<br>
Ben,<br>
<br>
<br>
<br>
They are technically the same thing _except_ that in common EM usage, "glow discharge" is done using ambient gases (i.e., you just pull a modest vacuum and create a plasma) while "plasma cleaning" is done using a specific mix of gases (Ar, O and with the
Gatan device H) instead of ambient gases (i.e., you flush the chamber with specific gases, then pull the vacuum and create the plasma).<br>
<br>
<br>
<br>
With more O in the plasma, the cleaning tends to be stronger (burns more material in the sample) and the Ar/O mix prevents side reactions that can be caused by the abundant N in the atmosphere.<br>
<br>
<br>
--<br>
David Gene Morgan<br>
Electron Microscopy Center<br>
047D Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="http://iubemcenter.indiana.edu">http://iubemcenter.indiana.edu</a><br>
________________________________<br>
From: 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of<span class="Apple-converted-space"> </span><a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a><span class="Apple-converted-space"> </span><<a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a>><br>
Sent: Tuesday, September 20, 2016 11:28 AM<br>
To:<span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
Subject: [3dem] glow discharge vs plasma cleaning<br>
<br>
Dear colleagues,<br>
<br>
I often hear people saying: „Did you treat your grid with glow discharge or with plasma cleaner?” Or : ”It is absolutely essential for application XY to pretreat the grid with a plasma cleaner and not by glow discharge!”.<br>
<br>
Can anyone explain what the difference between glow discharge and a plasma cleaning is?<br>
<br>
According to Wikipedia, this is the same thing (<a href="https://en.wikipedia.org/wiki/Glow_discharge">https://en.wikipedia.org/wiki/Glow_discharge</a>). The page starts with this sentence:<br>
“A glow discharge is a plasma<<a href="https://en.wikipedia.org/wiki/Plasma_(physics)">https://en.wikipedia.org/wiki/Plasma_(physics)</a>> formed by the passage of electric current<<a href="https://en.wikipedia.org/wiki/Electric_current">https://en.wikipedia.org/wiki/Electric_current</a>>
through a low-pressure gas”<br>
<br>
I look forward to an interesting debate.<br>
Ben<br>
<br>
Prof. Benoît Zuber<br>
Institute of Anatomy<br>
University of Bern<br>
Baltzerstrasse 2<br>
Postfach 922<br>
3000 Bern 9<br>
Switzerland<br>
Tel. +41 31 631 84 40<br>
<a href="mailto:benoit.zuber@ana.unibe.ch">benoit.zuber@ana.unibe.ch</a><br>
<a href="http://www.ana.unibe.ch/~exmo/">http://www.ana.unibe.ch/~exmo/</a><br>
<br>
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