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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US">Hi Bill<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US">Not sure what could be wrong. You will probably get much better help (from the programmer) if you post your question on the imod mailing list.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US">Best<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US">Ben<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:Calibri;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-family:Calibri;color:black">De : </span>
</b><span style="font-family:Calibri;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> au nom de Bill & Sue Tivol <wtivol@sbcglobal.net><br>
<b>Date : </b>jeudi, 8 septembre 2016 01:51<br>
<b>À : </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Objet : </b>[3dem] SIRT<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Hi All, <o:p></o:p></p>
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<p class="MsoNormal">I have a data set that I’d like to reconstruct with SIRT. I started etomo and successfully got through the final alignment step, resulting in a well-aligned tilt series. When I set up the Generate Tomogram step, clicking on SIRT, I chose
iterations 20 and 30 to save. The reconstruction failed, generating the error message shown below. I also tried a WBP reconstruction on the same data set, using the aligned tilt series from the SIRT reconstruction, and it gave a reasonable result. I do
not know how to interpret the error message. Could someone please let me know what I might do to get a successful SIRT reconstruction? BTW, I also tried to use the command line tomo3d -S -a filename.tlt -i filename.ali -o filename.srec with appropriate parameters
for thickness in z and iterations to save—a process I had used successfully on another computer—but I got a command-not-found error. Is there a way to install part of etomo to get access to that command? TIA for all help.<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:Helvetica;color:black">Yours,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:Helvetica;color:black">Bill<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:Helvetica;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><img width="451" height="330" id="_x0035_AAD4D9D-76AC-446D-B85C-54FD47853559" src="cid:image001.png@01D209B0.24873320"><o:p></o:p></p>
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