<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi All,<div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>I have a data set that I’d like to reconstruct with SIRT. I started etomo and successfully got through the final alignment step, resulting in a well-aligned tilt series. When I set up the Generate Tomogram step, clicking on SIRT, I chose iterations 20 and 30 to save. The reconstruction failed, generating the error message shown below. I also tried a WBP reconstruction on the same data set, using the aligned tilt series from the SIRT reconstruction, and it gave a reasonable result. I do not know how to interpret the error message. Could someone please let me know what I might do to get a successful SIRT reconstruction? BTW, I also tried to use the command line tomo3d -S -a filename.tlt -i filename.ali -o filename.srec with appropriate parameters for thickness in z and iterations to save—a process I had used successfully on another computer—but I got a command-not-found error. Is there a way to install part of etomo to get access to that command? TIA for all help.<br class=""><div class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class=""><span class="Apple-tab-span" style="white-space: pre; "> </span>Yours,</div><div class=""><span class="Apple-tab-span" style="white-space: pre; "> </span>Bill</div><div class=""><br class=""></div></span><br class="Apple-interchange-newline">
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