<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><div class=""></div><div class=""><br class=""></div><div class="">Could also be your humidifier is "raining too course”, maybe try a wet sponge in the Leica chamber instead of the humidifier.</div><div class=""><br class=""></div><div class="">Is your cryo-box cold and stable? Take images every minute in LM mode with the box visible, cool down the cold trap, you should see movement only over the first 30 minutes, then it needs to remain stable. If it keeps going back and forth between certain positions, it starts touching something, so it warms up, stops touching, cools down again etc.</div><div class=""><br class=""></div><div class="">An example of 30 min from our properly working Tecnai 12 cryo-box:</div><div class=""><br class=""></div><div class=""></div></body></html>