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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Hi Ben,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>After my Dropbox "crashed" I considered putting it on EMPIAR but this is an "unofficial" small subset of images. I will definitely deposit the complete dataset with the original movies once the paper is ready. Hopefully within a month.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>All the best,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Rado<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'>From:</span></b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu] <b>On Behalf Of </b>Benjamin Himes<br><b>Sent:</b> 05 July 2016 14:49<br><b>To:</b> 3dem@ncmir.ucsd.edu<br><b>Subject:</b> Re: [3dem] 3dem Digest, Vol 107, Issue 2<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p><div><div><p class=MsoNormal style='margin-bottom:12.0pt'>Dear Rado,<o:p></o:p></p></div><div><p class=MsoNormal style='margin-bottom:12.0pt'>Is there any reason in particular you elected to not share your data through the <a href="https://www.ebi.ac.uk/pdbe/emdb/empiar/">EMPIAR</a>?<br><br>"EMPIAR, the Electron Microscopy Pilot Image Archive, is a public resource for raw, 2D electron microscopy images. Here, you can browse, upload, and download and reprocess the thousands of raw, 2D images used to build a 3D structure."<o:p></o:p></p></div><div><p class=MsoNormal>-Ben H.<o:p></o:p></p></div></div><div><p class=MsoNormal><br clear=all><o:p></o:p></p><div><div><div><div><div><div><div><p class=MsoNormal style='text-align:justify'><span style='font-family:"Garamond",serif;color:#20124D'>*************************************</span><o:p></o:p></p><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D'>Benjamin Himes</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D'>Department of Structural Biology</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D;background:white'>University of Pittsburgh School of Medicine<br>2050 Biomedical Science Tower 3 (BST3).</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D'>3501 5th Ave, Pittsburgh, PA 15260</span><o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D;background:white'>(412) 648-7262 </span></b><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D;background:white'>(Office)</span><o:p></o:p></p></div><div><p class=MsoNormal><strong><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D;background:white'>(412)648-8998</span></strong><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D;background:white'> (Lab fax)</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#20124D;background:white'>*************************************</span><o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#274E13;background:white'>No trees were killed to send this message, but</span><o:p></o:p></p><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#274E13'>a large number of electrons were terribly inconvenienced.</span><o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><div><p class=MsoNormal style='line-height:18.0pt'><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#274E13'>It is an abiding passion of human beings to attempt to read and re-view history as an orderly progression of events running according to a well-coordinated and rational script. Don't be fooled.<o:p></o:p></span></p></div><div><p class=MsoNormal style='line-height:18.0pt'><span style='font-size:10.0pt;font-family:"Garamond",serif;color:#274E13'><o:p> </o:p></span></p></div></div></div></div></div></div></div></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal>On Tue, Jul 5, 2016 at 8:32 AM, <<a href="mailto:3dem-request@ncmir.ucsd.edu" target="_blank">3dem-request@ncmir.ucsd.edu</a>> wrote:<o:p></o:p></p><blockquote style='border:none;border-left:solid #CCCCCC 1.0pt;padding:0cm 0cm 0cm 6.0pt;margin-left:4.8pt;margin-right:0cm'><p class=MsoNormal>Send 3dem mailing list submissions to<br> <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br><br>To subscribe or unsubscribe via the World Wide Web, visit<br> <a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>or, via email, send a message with subject or body 'help' to<br> <a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a><br><br>You can reach the person managing the list at<br> <a href="mailto:3dem-owner@ncmir.ucsd.edu">3dem-owner@ncmir.ucsd.edu</a><br><br>When replying, please edit your Subject line so it is more specific<br>than "Re: Contents of 3dem digest..."<br><br><br>Today's Topics:<br><br> 1. Phase plate test dataset (Radostin Danev)<br> 2. Postdoctoral position - Advanced Microscopy and Modelling<br> (Eric Gilles Hanssen)<br> 3. Re: Phase plate test dataset (Radostin Danev)<br><br><br>----------------------------------------------------------------------<br><br>Message: 1<br>Date: Tue, 5 Jul 2016 10:38:33 +0200<br>From: Radostin Danev <<a href="mailto:danev@biochem.mpg.de">danev@biochem.mpg.de</a>><br>To: <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>Subject: [3dem] Phase plate test dataset<br>Message-ID: <08a401d1d698$9d39d0c0$d7ad7240$@<a href="http://biochem.mpg.de" target="_blank">biochem.mpg.de</a>><br>Content-Type: text/plain; charset="us-ascii"<br><br>Dear Colleagues,<br><br><br><br>As I mentioned at the 3DEM GRC I am happy to share a small subset of phase<br>plate images acquired with defocus.<br><br>I put the first 20 images of a 20S proteasome dataset on my Dropbox, here:<br><a href="https://www.dropbox.com/sh/omjjhwhhqsjvsm6/AAAAg32yTzc4_CvrjtdQI_Raa?dl=0" target="_blank">https://www.dropbox.com/sh/omjjhwhhqsjvsm6/AAAAg32yTzc4_CvrjtdQI_Raa?dl=0</a><br><br>There are also picked particle coordinates in *_automatch.star and CTF<br>parameters in *_ctffind3.log files.<br><br>Other data:<br><br>- accel. voltage 300 kV<br><br>- pixel size 1.054 A<br><br>- Cs 2.62 mm<br><br>- particle diameter 190 A<br><br><br><br>With D7 symmetry this subset of ~ 7,300 particles refines to ~ 2.9 A<br>resolution.<br><br><br><br>All the best,<br><br><br><br>Rado<br><br><br><br><br><br>===================================<br><br>Radostin Danev, Ph.D.<br><br>Max Planck Institute of Biochemistry<br>Department of Molecular Structural Biology<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany<br><br>e-mail: <a href="mailto:danev@biochem.mpg.de">danev@biochem.mpg.de</a><br><br>phone: <a href="tel:%2B49%20%2889%29%208578-2651">+49 (89) 8578-2651</a><br>fax: <a href="tel:%2B49%20%2889%29%208578-2641">+49 (89) 8578-2641</a><br><br>===================================<br><br><br><br><br><br>-------------- next part --------------<br>An HTML attachment was scrubbed...<br>URL: <<a href="http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160705/26bb81b7/attachment-0001.html" target="_blank">http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160705/26bb81b7/attachment-0001.html</a>><br><br>------------------------------<br><br>Message: 2<br>Date: Tue, 5 Jul 2016 10:39:48 +0000<br>From: Eric Gilles Hanssen <<a href="mailto:ehanssen@unimelb.edu.au">ehanssen@unimelb.edu.au</a>><br>To: "<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>Subject: [3dem] Postdoctoral position - Advanced Microscopy and<br> Modelling<br>Message-ID: <<a href="mailto:4E057941-24F1-43B7-B41B-2D14A3263E9A@unimelb.edu.au">4E057941-24F1-43B7-B41B-2D14A3263E9A@unimelb.edu.au</a>><br>Content-Type: text/plain; charset="windows-1252"<br><br>Dear all,<br>The University of Melbourne is advertising in new post doctoral position for advanced imaging see below.<br>Please contact Prof Leann Tilley for more info <a href="mailto:ltilley@unimelb.edu.au">ltilley@unimelb.edu.au</a><mailto:<a href="mailto:ltilley@unimelb.edu.au">ltilley@unimelb.edu.au</a>><br>Regards<br>Eric<br><br>Postdoctoral Research Fellow ? Advanced Microscopy<br>Department of Biochemistry and Molecular Biology, University of Melbourne<br><br>This position is funded by an Australian Research Council Laureate Fellowship Program. The vision of the program is to develop sophisticated and integrated bio-metrology (measurement) methods to study a complex cellular processes in the malaria parasite. You will work with a team of experts to develop and implement novel imaging methods, biophysical techniques and computational studies that will enable, for the first time, whole organism mapping and modelling of malaria parasites. The Program will work as an interdisciplinary collaboration for high-resolution bio-imaging and computational modelling. The host laboratory is situated in the Bio21 Molecular Science and Biotechnology Institute, on the University campus. You will work with colleagues from the University?s Departments of Biochemistry and Molecular Biology, Maths and Stats and from the School of Engineering.<br>Salary: $66,809* - $90,657 p.a. (*PhD Entry Level $84,458 p.a.) plus 9.5% superannuation<br>Job No: 0041023<br>For position information and to apply online go to <a href="http://about.unimelb" target="_blank">http://about.unimelb</a><<a href="http://about.unimelb.edu.au/careers" target="_blank">http://about.unimelb.edu.au/careers</a>>.<a href="http://edu.au/careers" target="_blank">edu.au/careers</a><<a href="http://about.unimelb.edu.au/careers" target="_blank">http://about.unimelb.edu.au/careers</a>>, and under the relevant option (?Current Staff? or ?Prospective Staff?) search by the job title or number.<br><a href="http://jobs.unimelb.edu.au/caw/en/job/888403/postdoctoral-research-fellow-advanced-microscopy" target="_blank">http://jobs.unimelb.edu.au/caw/en/job/888403/postdoctoral-research-fellow-advanced-microscopy</a><br><br><br><br>Assoc/Prof Eric Hanssen<br><br>Head - Bio21 Advanced Microscopy Facility<br>Principle Fellow - Department of Biochemistry and Molecular Biology<br><br>The University of Melbourne<br>Bio21 Institute - 30 flemington rd - VIC 3010<br><a href="tel:%2B61%203%208344%202449">+61 3 8344 2449</a><br><a href="http://www.microscopy.unimelb.edu.au" target="_blank">www.microscopy.unimelb.edu.au</a><<a href="http://www.microscopy.unimelb.edu.au" target="_blank">http://www.microscopy.unimelb.edu.au</a>><br><br><br><br><br>-------------- next part --------------<br>An HTML attachment was scrubbed...<br>URL: <<a href="http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160705/f331499c/attachment-0001.html" target="_blank">http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160705/f331499c/attachment-0001.html</a>><br><br>------------------------------<br><br>Message: 3<br>Date: Tue, 5 Jul 2016 14:31:35 +0200<br>From: Radostin Danev <<a href="mailto:danev@biochem.mpg.de">danev@biochem.mpg.de</a>><br>To: <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>Subject: Re: [3dem] Phase plate test dataset<br>Message-ID: <092301d1d6b9$2b29ab60$817d0220$@<a href="http://biochem.mpg.de" target="_blank">biochem.mpg.de</a>><br>Content-Type: text/plain; charset="us-ascii"<br><br>My Dropbox public links were suspended due to "excessive traffic". I<br>uploaded the dataset to our FTP server (could be a bit slow):<br><br><br><br><a href="http://bmftp.biochem.mpg.de" target="_blank">bmftp.biochem.mpg.de</a><br><br>user: MPIB<br><br>pass: biochemie<br><br><br><br>All the best,<br><br><br><br>Rado<br><br><br><br><br><br>From: 3dem [mailto:<a href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>] On Behalf Of Radostin Danev<br>Sent: 05 July 2016 10:39<br>To: <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>Subject: [3dem] Phase plate test dataset<br><br><br><br>Dear Colleagues,<br><br><br><br>As I mentioned at the 3DEM GRC I am happy to share a small subset of phase<br>plate images acquired with defocus.<br><br>I put the first 20 images of a 20S proteasome dataset on my Dropbox, here:<br><a href="https://www.dropbox.com/sh/omjjhwhhqsjvsm6/AAAAg32yTzc4_CvrjtdQI_Raa?dl=0" target="_blank">https://www.dropbox.com/sh/omjjhwhhqsjvsm6/AAAAg32yTzc4_CvrjtdQI_Raa?dl=0</a><br><br>There are also picked particle coordinates in *_automatch.star and CTF<br>parameters in *_ctffind3.log files.<br><br>Other data:<br><br>- accel. voltage 300 kV<br><br>- pixel size 1.054 A<br><br>- Cs 2.62 mm<br><br>- particle diameter 190 A<br><br><br><br>With D7 symmetry this subset of ~ 7,300 particles refines to ~ 2.9 A<br>resolution.<br><br><br><br>All the best,<br><br><br><br>Rado<br><br><br><br><br><br>===================================<br><br>Radostin Danev, Ph.D.<br><br>Max Planck Institute of Biochemistry<br>Department of Molecular Structural Biology<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany<br><br>e-mail: <a href="mailto:danev@biochem.mpg.de">danev@biochem.mpg.de</a><br><br>phone: <a href="tel:%2B49%20%2889%29%208578-2651">+49 (89) 8578-2651</a><br>fax: <a href="tel:%2B49%20%2889%29%208578-2641">+49 (89) 8578-2641</a><br><br>===================================<br><br><br><br><br><br>-------------- next part --------------<br>An HTML attachment was scrubbed...<br>URL: <<a href="http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160705/96ff3bba/attachment.html" target="_blank">http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160705/96ff3bba/attachment.html</a>><br><br>------------------------------<br><br>Subject: Digest Footer<br><br>_______________________________________________<br>3dem mailing list<br><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br><br><br>------------------------------<br><br>End of 3dem Digest, Vol 107, Issue 2<br>************************************<o:p></o:p></p></blockquote></div><p class=MsoNormal><o:p> </o:p></p></div></div></body></html>