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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Dear Colleagues,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>As I mentioned at the 3DEM GRC I am happy to share a small subset of phase plate images acquired with defocus.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>I put the first 20 images of a 20S proteasome dataset on my Dropbox, here: https://www.dropbox.com/sh/omjjhwhhqsjvsm6/AAAAg32yTzc4_CvrjtdQI_Raa?dl=0<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>There are also picked particle coordinates in *_automatch.star and CTF parameters in *_ctffind3.log files.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Other data:<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>- accel. voltage 300 kV<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>- pixel size 1.054 A<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>- Cs 2.62 mm<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>- particle diameter 190 A<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>With D7 symmetry this subset of ~ 7,300 particles refines to ~ 2.9 A resolution.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>All the best,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Rado<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-family:"Calibri",sans-serif;color:black'>===================================<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Calibri",sans-serif;color:black'>Radostin Danev, Ph.D.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Calibri",sans-serif;color:black'>Max Planck Institute of Biochemistry<br>Department of Molecular Structural Biology<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany</span><span style='font-size:9.0pt;font-family:"Calibri",sans-serif;color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>e-mail: danev@biochem.mpg.de<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Calibri",sans-serif;color:black'>phone: +49 (89) 8578-2651<br>fax: +49 (89) 8578-2641</span><span style='font-family:"Calibri",sans-serif;color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Calibri",sans-serif;color:black'>===================================<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p></div></body></html>