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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dear Johanna,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Buy a dry-shipper from Taylor-Wharton, this type of dewar is supposed to hold samples for ~ 17 days (it’s a CX100 here:
<a href="http://www.taylorwharton.com/assets/base/newsite/literature/cryoscience/TW-330CX-WEB.PDF">
http://www.taylorwharton.com/assets/base/newsite/literature/cryoscience/TW-330CX-WEB.PDF</a>). And indeed the specs say 17 days.
<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">These dry shippers are used all the time by people who send frozen grids to national facilities to collect EM data (NeCEN, eBic, etc…) and by dozens
of cryo-EM labs around the world…<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Cheers,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Sacha</span><span style="font-family:"Calibri","sans-serif";color:#1F497D"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Johanna Höög<br>
<b>Sent:</b> Tuesday, May 24, 2016 9:56 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Transport of frozen cryo-EM samples<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Dear EM colleagues,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">I wonder if anyone has experiences with transporting cryo-EM grids, so that the plunge freezing takes place at a different location from imaging? If you have
done this to positive effect, can you please give me some information on the equipment that you used and how long you transported the samples (in time)? Tips and tricks? Is it even possible to FedEx? <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Many thanks in advance, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Johanna <o:p></o:p></span></p>
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