<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
Dear Sjors,
<div class=""><br class="">
</div>
<div class="">Very nice video, indeed. And thanks for referring to Rado’s paper. </div>
<div class=""><br class="">
</div>
<div class="">Rado Pantelic still followed up with a later publication that might also be of interest. That appeared in Applied Physics Letters, which is a good journal in Nanosciences, but unfortunately not indexed in PubMed. </div>
<div class="">Appl. Phys. Lett. 104, 134103 (2014), <a href="http://dx.doi.org/10.1063/1.4870531" class="">http://dx.doi.org/10.1063/1.4870531</a></div>
<div class=""><br class="">
</div>
<div class="">Rado there describes that merely dipping a grid with pristine graphene into pyrene solution makes it hydrophilic, without loosing any of the amazing capabilities of graphene (extremely high electric conductivity, almost zero image background,
etc.). </div>
<div class=""><br class="">
</div>
<div class="">All the best,</div>
<div class=""><br class="">
</div>
<div class="">Henning.</div>
<div class=""><br class="">
</div>
<div class="">
<div class=""><span class="Apple-style-span" style="border-collapse: separate; font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class="">
<div class="">
<div class=""><span class="Apple-style-span" style="color: rgb(102, 102, 153); font-family: 'Stone Sans SC ITC TT'; font-size: 11px; ">Henning Stahlberg, PhD</span></div>
<div class=""><font class="Apple-style-span" color="#666699" face="'Stone Sans SC ITC TT'" size="3"><span class="Apple-style-span" style="font-size: 11px; ">Prof. for Structural Biology, C-CINA, Biozentrum, University Basel</span></font></div>
<div class=""><font class="Apple-style-span" color="#666699" face="'Stone Sans SC ITC TT'" size="3"><span class="Apple-style-span" style="font-size: 11px; ">Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland</span></font></div>
<div class=""><font class="Apple-style-span" color="#666699" face="'Stone Sans SC ITC TT'" size="3"><span class="Apple-style-span" style="font-size: 11px; "><a href="http://c-cina.org" class="">http://c-cina.org</a> | Tel. +41-61-387 32 62</span></font></div>
</div>
<div class=""><font class="Apple-style-span" color="#666699" face="'Stone Sans SC ITC TT'" size="3"><span class="Apple-style-span" style="font-size: 11px; "><br class="">
</span></font></div>
</div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></span><br class="Apple-interchange-newline">
</div>
<br class="">
<div>
<blockquote type="cite" class="">
<div class="">On Apr 28, 2016, at 16:17, Sjors Scheres <<a href="mailto:scheres@mrc-lmb.cam.ac.uk" class="">scheres@mrc-lmb.cam.ac.uk</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div class="">Dear colleagues,<br class="">
<br class="">
Recently, Thomas Martin and Anthony Fitzpatrick in my group, together with Andreas Boland from the Barford group, have successfully used graphene oxide as a support layer for cryo-EM grids on various projects (e.g. see
<a href="http://dx.doi.org/10.1038/ncomms11293" class="">http://dx.doi.org/10.1038/ncomms11293</a>). Graphene oxide may be used, much like a thin film of amorphous carbon, to concentrate particles on the grid, to modify orientational distributions, or to convince
particles to go over the holes. However, the background signal from a single layer of graphene oxide is significantly less than for a thin film of amorphous carbon, making it better suited for small complexes.<br class="">
<br class="">
The use of graphene oxide for this purpose was first proposed by Rado Pantelic et al. in the Plitzko group (see
<a href="http://dx.doi.org/10.1016/j.jsb.2009.12.020" class="">http://dx.doi.org/10.1016/j.jsb.2009.12.020</a>). Thomas, Andreas and Anthony made minor modifications to their procedure, i.e. they introduced washing steps to have a more reproducible coverage
of single graphene oxide layers. In the hope that this procedure is useful for others, Thomas has kindly prepared a video on how to prepare these grids. This procedure takes only a few minutes and is generally perceived as much easier than making and depositing
a thin film of amorphous carbon. The video is available through figshare: <a href="http://dx.doi.org/10.6084/m9.figshare.3178669" class="">
http://dx.doi.org/10.6084/m9.figshare.3178669</a>. Please direct questions about the practicalities of this method directly to Thomas (CC), and don't forget to cite the Pantelic paper when using graphene oxide grids.<br class="">
<br class="">
Best wishes,<br class="">
Sjors<br class="">
<br class="">
-- <br class="">
Sjors Scheres<br class="">
MRC Laboratory of Molecular Biology<br class="">
Francis Crick Avenue, Cambridge Biomedical Campus<br class="">
Cambridge CB2 0QH, U.K.<br class="">
tel: +44 (0)1223 267061<br class="">
<a href="http://www2.mrc-lmb.cam.ac.uk/groups/scheres" class="">http://www2.mrc-lmb.cam.ac.uk/groups/scheres</a><br class="">
<br class="">
_______________________________________________<br class="">
3dem mailing list<br class="">
3dem@ncmir.ucsd.edu<br class="">
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br class="">
</div>
</div>
</blockquote>
</div>
<br class="">
</div>
</body>
</html>