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<p class="MsoNormal"><font size="3">Dear 3dem colleagues,<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">An NIH-funded postdoctoral position to study platelet mechanosensory complexes is available in the Li lab (<a href="http://openwetware.org/wiki/Renhao_Li_Lab">http://openwetware.org/wiki/Renhao_Li_Lab</a>) in collaboration
with the Wright lab (<a href="http://electronmicroscopy.emory.edu/"><span style="color:windowtext">http://electronmicroscopy.emory.edu</span></a>). The available position is for a structural and biophysical study of the mechanosensory complex in the platelet
membrane by cutting-edge membrane protein biochemistry and cryo-EM. Recent studies have shown that this mechanosensory complex plays an important role in many aspects of platelet physiology and related pathophysiology.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">Publications applicable to the projects and methods associated with the position: <o:p></o:p></font></p>
<p class="MsoNormal"><font size="3">W. Zhang, W. Deng, L. Zhou, Y. Xu, W. Yang, X. Liang, Y. Wang, J. D. Kulman, X. F. Zhang, R. Li. (2015). Identification of a juxtamembrane mechanosensitive domain in the platelet mechanosensor glycoprotein Ib-IX complex.
<i>Blood</i> 125: 562-9 (Commentary in <i>Blood</i> 125: 423-424).<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">R. Li, J. Emsley. (2013). The organizing principle of platelet glycoprotein Ib-IX-V complex.
<i>J. Thromb. Haemost.</i> 11: 605-14.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">J. D. Strauss, J. E. Hammonds, H. Yi, L. Ding, P. W. Spearman, and E. R. Wright. Three-Dimensional Structural Characterization of HIV-1 Tethered to Human Cells.
<i>Journal of Virology</i>. 2016: 90 (3): 1507-1521.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">H. Yi, J. D. Strauss, Z. Ke, E. Alonas, R. S. Dillard, C. M. Hampton, K. M. Lamb, J. E. Hammonds, P. J. Santangelo, P. W. Spearman and E. R. Wright. Native Immunogold Labeling of Cell Surface Proteins and Viral Glycoproteins
for Cryo-Electron Microscopy and Cryo-Electron Tomography Applications. <i>Journal of Histochemistry and Cytochemistry</i>. 2015: 63 (10): 780-792.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">R. C. Guerrero-Ferreira, and E. R. Wright. Zernike Phase Contrast Cryo-Electron Tomography Of Whole Bacterial Cells.
<i>Journal of Structural Biology</i>. 2014: 185 (1): 129-133.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">The Li laboratory is within the Aflac Cancer and Blood Disorders Center and fully equipped for all aspects of molecular biology and membrane protein biochemistry. The Wright lab is within the Division of Pediatric Infectious
Disease and is equipped for all aspects of advanced imaging, to include: a JEOL JEM-2200FS 200 kV FEG TEM with an in-column energy filter, phase plate system, Direct Electron DE-20 direct electron detector, and Gatan 4kx4k CCD camera. For cryo-CLEM development
and experiments, a Leica cryo-CLEM system has recently been installed. The laboratory also has an FEI Vitrobot, a Gatan CP3 and a manual grid plunge freezer; a Leica cryo-ultramicrotome. The groups have access to all the resources available in the Emory University
EM Core Facility, such as: a JEOL JEM-1400 120 kV TEM with Gatan 2kx2k CCD camera; a Bal-Tec high-pressure freezer; and Leica freeze substitution system.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">Candidates should have an interest in structural biology and hematology; excellent communication skills; and enjoy working as part of a dynamic, collaborative and diverse research team. Background experience in one (or more)
of the following fields is desirable: molecular biology, biochemistry, (cryo) electron microscopy, and/or protein crystallography.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">Applicants should include a cover letter describing research experience and interests, curriculum vitae, and names and contact information for three references.<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">Dr. Elizabeth R. Wright<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3">Email: erwrigh@emory.edu<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">Dr. Renhao Li<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3">Email: renhao.li@emory.edu<o:p></o:p></font></p>
<p class="MsoNormal"><font size="3"> </font></p>
<p class="MsoNormal"><font size="3">Applications will be reviewed as they are received and until the position is filled.<o:p></o:p></font></p>
<!--EndFragment--></div>
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