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Hi,<br>
Unfortunately, there are no good tutorials. Also, the more that I
learn the more I realize that it is not as simple as I originally
assumed. I would suggest reading three papers as a start:<br>
<br>
<meta name="Title" content="">
<p class="MsoNormal"><span style="font-family:"Bookman Old
Style"">Egelman, E.H.
(2010), “Reconstruction of Helical Filaments and Tubes”, Methods
in Enzymology <b style="mso-bidi-font-weight:normal">482</b>,<span
style="mso-spacerun:yes">
</span>167-183.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Bookman Old
Style""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Bookman Old
Style"">Egelman, E.H. (2014).
“Ambiguities in helical reconstruction”. eLife 3:e04969
doi:10.7554/eLife.04969.<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-family:"Bookman Old
Style"">Egelman, E.H.
(2015). “Three-dimensional reconstruction of helical polymers”,
Archives of
Biochemistry and Biophysics <b
style="mso-bidi-font-weight:normal">581</b>,
54-58.</span><o:p></o:p></p>
<br>
Regards,<br>
Ed<br>
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<br>
<div class="moz-cite-prefix">On 2/29/16 2:58 PM, Johannes Haataja
wrote:<br>
</div>
<blockquote cite="mid:1456775920.14296.57.camel@tachyonzeta"
type="cite">
<pre wrap="">Dear all,
thank you for the replies. I now have an older version of spider.
Regarding IHRSR Prof. Egelman - what would the recommended way/tutorial
for learning to use IHRSR?
My best,
- J.
P.S. I guess ideally one would just read an article about the theory and
unix/linux man-pages of relevant command line tools and then inductively
reason how one must proceed to apply the method to the problem at hand.
Since I lack such a tenacity, I usually look for tutorials in order to
understand how the softwares/black boxes work. Also, I imagine that for
helical reconstruction, like for any inverse problem, there are many
different methods for recovering the quantit(y/ies) of interest and that
people usually are hesitant to openly aside with particular approach may
it be the right one or obviously the wrong one (e.g. Bayesian vs.
Frequentist interpretation of statistics) ;).
ma, 2016-02-29 kello 12:13 -0500, Michael Radermacher kirjoitti:
</pre>
<blockquote type="cite">
<pre wrap="">I would contact the people in Albany and also
discuss with them the problem you are having
with your version.
Michael
On 2/29/2016 11:46 AM, Johannes Haataja wrote:
</pre>
<blockquote type="cite">
<pre wrap="">Hi,
does anyone know where to obtain old versions of SPIDER, namely v.
21.02? The oldest from download page is 21.11. The reason for asking is
that I need and older SPIDER version to test IHRSR++ v. 1.5 tutorial
<a class="moz-txt-link-freetext" href="http://cryoem.ucsd.edu/wikis/software/start.php?id=ihrsr">http://cryoem.ucsd.edu/wikis/software/start.php?id=ihrsr</a>
to exclude the possibility that the errors I run into (in the final
reconstruction step) have something to do with SPIDER version.
My best,
- J.
_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
</pre>
</blockquote>
<pre wrap="">
_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
</pre>
</blockquote>
<pre wrap="">
_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
</pre>
</blockquote>
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<p class="MsoNormal">Edward H. Egelman, Ph.D.<u5:p></u5:p></p>
<p class="MsoNormal">Harrison Distinguished Professor<u5:p></u5:p></p>
<p class="MsoNormal">Dept. of Biochemistry and Molecular
Genetics<u5:p></u5:p></p>
<p class="MsoNormal">University of Virginia<u5:p></u5:p><o:p></o:p></p>
<p class="MsoNormal"><u5:p> </u5:p></p>
<p class="MsoNormal">President<u5:p></u5:p></p>
<p class="MsoNormal">Biophysical Society<u5:p></u5:p><o:p></o:p></p>
<p class="MsoNormal"><u5:p> </u5:p></p>
<p class="MsoNormal"><span class="GramE">phone</span>:
434-924-8210<u5:p></u5:p></p>
<p class="MsoNormal"><span class="GramE">fax</span>:
434-924-5069<u5:p></u5:p></p>
<p class="MsoNormal"><a class="moz-txt-link-abbreviated" href="mailto:egelman@virginia.edu">egelman@virginia.edu</a><u5:p></u5:p></p>
<p class="MsoNormal"><a
href="http://www.people.virginia.edu/%7Eehe2n"><a class="moz-txt-link-freetext" href="http://www.people.virginia.edu/~ehe2n">http://www.people.virginia.edu/~ehe2n</a></a></p>
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